The use of dried spent yeast as a low-cost nitrogen supplement in ethanol fermentation from sweet sorghum juice under very high gravity conditions
Open accessAbstract
Dried spent yeast (DSY) was used as a low-cost nitrogen supplement for ethanol fermentation from sweet sorghum juice under very high gravity (VHG) conditions by Saccharomyces cerevisiae NP 01. The fermentation was carried out at 30ºC in a 5-litre bioreactor. The results showed that DSY promoted ethanol production efficiencies. The ethanol concentration ( P ), productivity ( Q p ) and yield ( Y p/s ) of the sterile juice (total sugar of 280 g l -1 ) supplemented with 8 g l -1 of DSY were not different from those supplemented with yeast extract and/or peptone at the same amount. The initial yeast cell concentration of 5 x 10 7 cells ml -1 was found to be optimal for scale-up ethanol production. In addition, an increase in sugar concentration in inoculum preparation medium (from 10 to 100 g l -1 ) improved the ability of the inoculum to produce ethanol under the VHG conditions. When S. cerevisiae NP 01 grown in the juice containing 100 g l -1 of total sugar was used as the inoculum for ethanol fermentation, the P , Q p and Y p/s obtained were 108.98 ± 1.16 g l -1 , 2.27 ± 0.06 g l -1 h -1 and 0.47 ± 0.01 g g -1 , respectively. Similar results were also observed when the ethanol fermentation was scaled up to a 50-litre bioreactor under the same conditions. The cost of the sweet sorghum for ethanol production was US$ 0.63 per litre of ethanol. These results clearly indicate the high potential of using sweet sorghum juice supplemented with DSY under VHG fermentation for ethanol production in industrial applications.
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Brewer's spent yeast contains high levels of nitrogen and essential minerals, aiding the growth of fermenting yeast.
“Brewer's spent yeast contains high levels of nitrogen and essential minerals, aiding the growth of fermenting yeast.”
From Brewer’s Spent Yeast (BSY), an Underutilized Brewing By-Product (2020) · cites this paper as [102]
In comparison to yeast extracts and peptone, the results of using BSY as a nutritional supplement for fermentations were very similar.
“In comparison to the use of yeast extracts and peptone, more commonly used nutritional supplements for fermentations, the results were very similar [102].”
From Brewer’s Spent Yeast (BSY), an Underutilized Brewing By-Product (2020) · cites this paper as [102]
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- Authors
- Laopaiboon, L., Sridee, W., Jaisil, P., & Laopaiboon, P.
- Affiliations
- Khon Kaen University (TH)
- Venue
- Electronic Journal of Biotechnology
- Publisher
- Elsevier BV
- Published
- 2011-11-14
- Volume
- 14
- Issue
- 6
- ISSN
- 0717-3458 (ISSN-L)
- OpenAlex
- W2008791248
- Cited by
- 40
- References
- 40
- Citations by year
- 2025: 1 · 2024: 4 · 2023: 1 · 2022: 1 · 2021: 7 · 2020: 5 · 2019: 4 · 2018: 4 · 2017: 3 · 2016: 2 · 2015: 1 · 2014: 2 · 2013: 2 · 2012: 2
- FWCI
- 1.14
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc-nd
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- Locations
- Electronic Journal of Biotechnology (publishedVersion, cc-by-nc-nd)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
- Bioline International (Bioline International) (submittedVersion)
- Americanae (AECID Library) (submittedVersion, other-oa)
- Funders
- Khon Kaen University
- National Research Council of Thailand
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Agricultural and Biological Sciences › Food Science › Fermentation and Sensory Analysis
- Keywords
- nitrogen supplementation, alcoholic fermentation, sweet sorghum juice, very high gravity fermentation, Saccharomyces cerevisiae, yeast extract replacement, inoculum preparation, ethanol yield, ethanol productivity, scale-up
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