Optimization of Nitrogen and Metal Ions Supplementation for Very High Gravity Bioethanol Fermentation from Sweet Sorghum Juice Using an Orthogonal Array Design
Open accessAbstract
Optimization of four parameters, i.e., zinc (Zn2+), magnesium (Mg2+), manganese (Mn2+) and yeast extract for bioethanol production from sweet sorghum juice by Saccharomyces cerevisiae NP 01 under very high gravity (VHG, 270 g·L−1 of total sugar) conditions was performed using an L9 (34) orthogonal array design. The fermentation was carried out at 30 °C in 500-mL air-locked Erlenmeyer flasks at the agitation rate of 100 rpm and the initial yeast cell concentration in the juice was approximately 5 × 107 cells·mL−1. The results showed that the order of influence was yeast extract > Mn2+ > Zn2+ > Mg2+ and the optimum nutrient concentrations for the ethanol fermentation were Zn2+, 0.01; Mg2+, 0.05; Mn2+, 0.04; and yeast extract, 9 g·L−1. The verification experiments under the optimum condition clearly indicated that the metals and nitrogen supplementation improved ethanol production efficiency under the VHG fermentation conditions. The ethanol concentration (P), yield (Yp/s) and productivity (Qp) were 120.58 ± 0.26 g·L−1, 0.49 ± 0.01 and 2.51 ± 0.01 g·L−1·h−1, respectively, while in the control treatment (without nutrient supplement) P, Yp/s and Qp were only 93.45 ± 0.45 g·L−1, 0.49 ± 0.00 and 1.30 ± 0.01 g·L−1·h−1, respectively.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Fermentable sugars such as glucose, fructose, maltose, and rhamnose are used as substrates by microorganisms, which convert them to ethanol and CO2.
“Fermentable sugars, such as glucose, fructose, maltose, and rhamnose, are used as substrates by microorganisms in the fermentation process, which converts them to ethanol and CO2 [121, 122].”
From The promising future of microalgae: Current status, challenges, and optimization of a sustainable and renewable industry for biofuels, feed, and other products (2018) · cites this paper as [122]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Ethanol fermentation technologies from sugar and starch feedstocks
Bai, F., Anderson, W. A., & Moo‐Young, M. (2007) · Biotechnology Advances · doi:10.1016/j.biotechadv.2007.09.002 · Cited by 961 · see citing works
Yeast Physiology and Biotechnology
Walker, G. M. (1998) · Medical Entomology and Zoology · Cited by 628 · see citing works
Zoecklein, B. W., Fugelsang, K. C., Gump, B. H., & Nury, F. S. (1999) · Springer eBooks · doi:10.1007/978-1-4757-6967-8 · Cited by 565 · see citing works
Bely, M., Sablayrolles, J.-M., & Barre, P. (1990) · Journal of Fermentation and Bioengineering · doi:10.1016/0922-338x(90)90057-4 · Cited by 479 · see citing works
Compatible Solutes and Extreme Water Stress in Eukaryotic Micro-Organisms
Brown, A. D. (1978) · Advances in microbial physiology/Advances in Microbial Physiology · doi:10.1016/s0065-2911(08)60058-2 · Cited by 420 · see citing works
Laopaiboon, L., Nuanpeng, S., Srinophakun, P., Klanrit, P., & Laopaiboon, P. (2009) · Bioresource Technology · doi:10.1016/j.biortech.2009.03.046 · Cited by 284 · see citing works
Self-citationFeatures of sweet sorghum juice and their performance in ethanol fermentation
Wu, X., Staggenborg, S., Propheter, J. L., Rooney, W. L., Yu, J., & Wang, D. (2009) · Industrial Crops and Products · doi:10.1016/j.indcrop.2009.10.006 · Cited by 263 · see citing works
Zoecklein, B. W., Fugelsang, K. C., Gump, B. H., et al. (1995) · Springer eBooks · doi:10.1007/978-1-4757-6978-4 · Cited by 260 · see citing works
Thomas, K. C., & Ingledew, W. M. (1990) · Applied and Environmental Microbiology · doi:10.1128/aem.56.7.2046-2050.1990 · Cited by 236 · see citing works
Influence of magnesium ions on heat shock and ethanol stress responses of Saccharomyces cerevisiae
Birch, R. M., & Walker, G. M. (2000) · Enzyme and Microbial Technology · doi:10.1016/s0141-0229(00)00159-9 · Cited by 225 · see citing works
Beltran, G., Esteve‐Zarzoso, B., Rozés, N., Mas, A., & Guillamón, J. M. (2005) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf0487001 · Cited by 218 · see citing works
Siqueira, P. F., Karp, S. G., Carvalho, J. C., Sturm, W., Rodríguez-León, J. A., Tholozan, J.-L., Singhania, R. R., Pandey, A., & Soccol, C. R. (2008) · Bioresource Technology · doi:10.1016/j.biortech.2008.03.037 · Cited by 203 · see citing works
Chae, H. J., Joo, H., & In, M. (2001) · Bioresource Technology · doi:10.1016/s0960-8524(00)00102-4 · Cited by 188 · see citing works
Heard, G. M., & Fleet, G. H. (1988) · Journal of Applied Bacteriology · doi:10.1111/j.1365-2672.1988.tb04312.x · Cited by 185 · see citing works
Mecozzi, M. (2005) · Chemometrics and Intelligent Laboratory Systems · doi:10.1016/j.chemolab.2005.04.005 · Cited by 177 · see citing works
LIU, R., & SHEN, F. (2008) · Bioresource Technology · doi:10.1016/j.biortech.2007.01.009 · Cited by 173 · see citing works
Thomas, K. C., Hynes, S. H., & Ingledew, W. M. (1996) · Process Biochemistry · doi:10.1016/0032-9592(95)00073-9 · Cited by 156 · see citing works
Pereira, F. B., Guimarães, P. M. R., Teixeira, J. A., & Domingues, L. (2010) · Bioresource Technology · doi:10.1016/j.biortech.2010.04.082 · Cited by 154 · see citing works
Bai, F., Chen, L. J., Zhang, Z., Anderson, W. A., & Moo‐Young, M. (2004) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2004.01.017 · Cited by 143 · see citing works
Bayrock, D. P., & Ingledew, W. M. (2001) · Journal of Industrial Microbiology & Biotechnology · doi:10.1038/sj.jim.7000167 · Cited by 142 · see citing works
Bely, M., Rinaldi, A., & Dubourdieu, D. (2003) · Journal of Bioscience and Bioengineering · doi:10.1016/s1389-1723(04)70141-3 · Cited by 139 · see citing works
Bafrncová, P., Šmogrovičová, D., Sláviková, I., Pátková, J., & Dömény, Z. (1999) · Biotechnology Letters · doi:10.1023/a:1005436816047 · Cited by 129 · see citing works
Wang, X. D., Bohlscheid, J. C., & Edwards, C. G. (2003) · Journal of Applied Microbiology · doi:10.1046/j.1365-2672.2003.01827.x · Cited by 125 · see citing works
Siderius, M. H., Wuytswinkel, O. V., Reijenga, K. A., et al. (2000) · Molecular Microbiology · doi:10.1046/j.1365-2958.2000.01955.x · Cited by 116 · see citing works
Thomas, K. C., Hynes, S. H., & Ingledew, W. M. (1994) · Applied and Environmental Microbiology · doi:10.1128/aem.60.5.1519-1524.1994 · Cited by 115 · see citing works
Davila-Gomez, F. J., Chuck‐Hernández, C., Pérez‐Carrillo, E., et al. (2011) · Industrial Crops and Products · doi:10.1016/j.indcrop.2010.12.022 · Cited by 115 · see citing works
Schepers, A. W., Thibault, J., & Lacroix, C. (2002) · Enzyme and Microbial Technology · doi:10.1016/s0141-0229(01)00465-3 · Cited by 107 · see citing works
Refining bioethanol from stalk juice of sweet sorghum by immobilized yeast fermentation
Liu, R., Li, J., & Shen, F. (2007) · Renewable Energy · doi:10.1016/j.renene.2007.05.046 · Cited by 97 · see citing works
Zhao, X., Xue, C., Ge, X., Yuan, W., Wang, J. Y., & Bai, F. (2008) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2008.08.013 · Cited by 95 · see citing works
Jørgensen, H. (2008) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-008-8456-0 · Cited by 90 · see citing works
Zinc, Copper and Manganese Enrichment in Yeast Saccharomyces cerevisae
Stehlik-Tomas, V., Stanzer, D., Grba, S., Vahčić, N., & Zetić, V. G. (2004) · Hrčak Portal of scientific journals of Croatia (University Computing Centre) · Cited by 86 · see citing works
Optimization of an ethanol production medium in very high gravity fermentation
Wang, F.-Q., Gao, C., Yang, C., et al. (2006) · Biotechnology Letters · doi:10.1007/s10529-006-9220-6 · Cited by 76 · see citing works
Ethanol production in solid substrate fermentation using thermotolerant yeast
Sree, N. K., Sridhar, M., Rao, L. V., et al. (1999) · Process Biochemistry · doi:10.1016/s0032-9592(98)00074-0 · Cited by 71 · see citing works
Wang, S., Thomas, K. C., Sosulski, K., et al. (1999) · Process Biochemistry · doi:10.1016/s0032-9592(98)00097-1 · Cited by 69 · see citing works
Fuel Alcohol Production: Optimization of Temperature for Efficient Very-High-Gravity Fermentation
Jones, A. M., & Ingledew, W. M. (1994) · Applied and Environmental Microbiology · doi:10.1128/aem.60.3.1048-1051.1994 · Cited by 68 · see citing works
Improved ethanol yields through supplementation with excess assimilable nitrogen
O'Connor-Cox, E. S. C., Paik, J., & Ingledew, W. M. (1991) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/bf01575590 · Cited by 58 · see citing works
Bvochora, J. M., Read, J. S., & Zvauya, R. (2000) · Industrial Crops and Products · doi:10.1016/s0926-6690(99)00029-1 · Cited by 55 · see citing works
Khongsay, N., Laopaiboon, L., Jaisil, P., et al. (2012) · Energies · doi:10.3390/en5030561 · Cited by 53 · see citing works
Self-citationAccumulation of magnesium ions during fermentative metabolism in Saccharomyces cerevisiae
Walker, G. M., & Maynard, A. I. (1997) · Journal of Industrial Microbiology & Biotechnology · doi:10.1038/sj.jim.2900356 · Cited by 50 · see citing works
Sergio, O., Chuck-Hernndez, C., Prez-Carrillo, E., et al. (2012) · Bioethanol · doi:10.5772/20489 · Cited by 47 · see citing works
Improving ethanol tolerance of a self-flocculating yeast by optimization of medium composition
Xue, C., Zhao, X., Yuan, W., et al. (2008) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-008-9739-x · Cited by 45 · see citing works
Larsson, C., & Gustafsson, L. (1987) · Archives of Microbiology · doi:10.1007/bf00406133 · Cited by 45 · see citing works
Current Aspects of Fuel Ethanol Production in Brazil
Laluce, C. (1991) · Critical Reviews in Biotechnology · doi:10.3109/07388559109040620 · Cited by 44 · see citing works
Hu, C.-K., Bai, F., & An, L. (2003) · Biotechnology Letters · doi:10.1023/a:1024583503274 · Cited by 44 · see citing works
Laopaiboon, L., Sridee, W., Jaisil, P., & Laopaiboon, P. (2011) · Electronic Journal of Biotechnology · doi:10.2225/vol14-issue6-fulltext-5 · Cited by 40 · see citing works
Self-citationThe Effect of Fermentation Temperature on the Growth Kinetics of Wine Yeast Species
Şener, A., Canbaş, A., & Ünal, M. Ü. (2007) · DergiPark (Istanbul University) · Cited by 36 · see citing works
Effect of zinc on ethanol production by two Thermoanaerobacter strains
Avcı, A., & Dönmez, S. (2005) · Process Biochemistry · doi:10.1016/j.procbio.2005.11.007 · Cited by 35 · see citing works
Fuel ethanol production from sweet sorghum using repeated-batch fermentation
Chohnan, S., Nakane, M., Rahman, M., et al. (2011) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2010.12.014 · Cited by 33 · see citing works
Zhang, A., & Chen, X. (2008) · Chinese Journal of Chemical Engineering · doi:10.1016/s1004-9541(08)60130-5 · Cited by 30 · see citing works
Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant
Milošević, S. M., Subotić, A. R., Bulajić, A., et al. (2001) · Electronic Journal of Biotechnology · doi:10.2225/vol14-issue1-fulltext-5 · Cited by 30 · see citing works
Volatile Compounds of a Traditional Thai Rice Wine
Sirisantim, L., Laopaiboon, L., Danvirutai, P., et al. (2008) · Biotechnology(Faisalabad) · doi:10.3923/biotech.2008.505.513 · Cited by 22 · see citing works
Self-citationLaopaiboon, L., Nuanpeng, S., Srinophaku, P., Klanrit, P., & Laopaiboon, P. (2008) · Biotechnology(Faisalabad) · doi:10.3923/biotech.2008.493.498 · Cited by 22 · see citing works
Self-citationAs Brazil Fills Up on Ethanol, It Weans Off Energy Imports
Luhnow, D. (2006) · Cited by 20 · see citing works
Influence of heat shock on glycerol production in alcohol fermentation
Berovič, M., Pivec, A., Košmerl, T., et al. (2007) · Journal of Bioscience and Bioengineering · doi:10.1263/jbb.103.135 · Cited by 19 · see citing works
Bravo, V., Camacho, F., Sánchez, S., et al. (1995) · Journal of Fermentation and Bioengineering · doi:10.1016/0922-338x(95)94749-h · Cited by 19 · see citing works
Kinetics of spontaneous wine production
Özilgen, M., Çelik, M., & Bozoǧlu, T. F. (1991) · Enzyme and Microbial Technology · doi:10.1016/0141-0229(91)90137-y · Cited by 13 · see citing works
Lima, M. A. P., & Natalense, A. P. P. (2012) · InTech eBooks · doi:10.5772/850 · Cited by 9 · see citing works
Flagged first, then by citations
Research works printed but not linked: 4
- Pinheiro Lima, M.A., Policastro Natalence, A.P., Eds.; In Tech: London, UK, 2012; Chapter 3, pp. 51-74.
- Minteer, S.D. Alcoholic Fuels; CRC Press Taylor and Francis group: London, UK, 2006.
- Sridee, W.; Laopaiboon, L.; Jaisil, P.; Laopaiboon, P. The use of dried spent yeast as a low c-cost nitrogen supplementation from sweet sorghum juice under very high gravity condition. Electron. J. Biotechnol. 2011, 14, 1-15.
- Jangchud, A. Product optimization. In Statistics for Product Development and Application; Kasetsart University: Bangkok, Thailand, 2006; pp. 241-288.
Cited by
Sorted by citations
Khan, M. I., Shin, J. H., & Kim, J. D. (2018) · Microbial Cell Factories · doi:10.1186/s12934-018-0879-x · Cited by 2,082 · see citing works
Comparative Review on the Production and Purification of Bioethanol from Biomass: A Focus on Corn
Assaf, J. C., Mortada, Z., Rezzoug, S.-A., Maache-Rezzoug, Z., Debs, E., & Louka, N. (2024) · Processes · doi:10.3390/pr12051001 · Cited by 51 · see citing works
- Authors
- Deesuth, O., Laopaiboon, P., Jaisil, P., & Laopaiboon, L.
- Affiliations
- Khon Kaen University (TH)
- Corresponding author
- Laopaiboon, Lakkana
- Venue
- Energies
- Publisher
- MDPI AG
- Published
- 2012-08-24
- Volume
- 5
- Issue
- 9
- Pages
- 3178-3197
- ISSN
- 1996-1073 (ISSN-L)
- OpenAlex
- W2042086945
- Cited by
- 50
- References
- 57
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 4 · 2023: 4 · 2022: 3 · 2021: 6 · 2020: 9 · 2019: 4 · 2018: 5 · 2017: 4 · 2016: 1 · 2015: 2 · 2014: 3 · 2013: 3
- FWCI
- 1.46
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Energies (publishedVersion, cc-by)
- RePEc: Research Papers in Economics (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Higher Education Research Promotion
- Khon Kaen University
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Agricultural and Biological Sciences › Food Science › Fermentation and Sensory Analysis
- Keywords
- bioethanol production, very high gravity fermentation, sweet sorghum juice, Saccharomyces cerevisiae, zinc supplementation, magnesium supplementation, manganese supplementation, yeast extract, nitrogen supplementation, fermentation optimization, ethanol yield, ethanol productivity
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
