New biotechnological applications of coconuts
Open accessAbstract
Coconut is a major crop of many poor nations. The present paper shows with inexpensive fluorescence microscopy of the enhanced green fluorescent protein (EGFP), that coconut water has the capability of synthesizing proteins from recombinant DNA vectors. After only 4 hrs of 100 pM application of the EGFP plasmid (pEGFP), a strong fluorescent signal was detected with an off-the-shelf, low-tech CCD webcam. Since natural coconut water is sterile, this heat-stable delivery system may be a good option for the dissemination of food supplements and pharmaceuticals in poor nations. As expensive substances could be expressed in coconut water, through bioreactor technology or micropropagation, the concepts presented here may prove invaluable to the economic and social advancement of many developing countries.
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Coconut water appears able to support the synthesis of proteins from recombinant DNA vectors.
“Coconut water appears to be able to support the synthesis of proteins from recombinant DNA vectors [26].”
From Coconut water uses, composition and properties: a review (2012) · cites this paper as [26]
Cites
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Coconut water uses, composition and properties: a review
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- Authors
- Bustamante, J. O.
- Affiliations
- Universidade Federal de Sergipe (BR)
- Corresponding author
- Bustamante, José O.
- Venue
- Electronic Journal of Biotechnology
- Publisher
- Elsevier BV
- Published
- 2004-04-15
- Volume
- 7
- Issue
- 1
- ISSN
- 0717-3458 (ISSN-L)
- OpenAlex
- W4213136604
- Cited by
- 3
- References
- 10
- Citations by year
- 2022: 1 · 2012: 1
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc-nd
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- Locations
- Electronic Journal of Biotechnology (publishedVersion, cc-by-nc-nd)
- Funders
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Westfälische Wilhelms-Universität Münster
- Instituto Millenium
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
- Physical Sciences › Chemistry › Inorganic Chemistry › Coconut Research and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Keywords
- coconut water, enhanced green fluorescent protein, recombinant protein expression, plasmid DNA delivery, bioreactor technology, micropropagation, dietary supplements
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