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Isolation of Cryptosporidium Oocysts and Sporozoites Using Discontinuous Sucrose and Isopycnic Percoll Gradients
Open accessAbstract
Techniques for the large-scale isolation of Cryptosporidium oocysts and sporozoites, obtained from the feces of experimentally infected Holstein calves, were developed employing discontinuous sucrose gradients and isopycnic Percoll gradients. The oocyst recovery method utilized 2 sequential discontinuous sucrose gradients followed by 1 Percoll gradient. Recovered oocysts were essentially free of debris and bacteria and represented 34% of the original oocyst suspension. Sporozoites were recovered from excystation mixtures on a single Percoll gradient. Sixty-three percent of the original sporozoites were recovered with 2.2% contamination by intact oocysts and virtually no oocyst walls.
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Citations
Cited by
Virulence of Three Distinct Cryptosporidium parvum Isolates for Healthy Adults
Okhuysen, P. C., Chappell, C. L., Crabb, J. H., Sterling, C. R., & DuPont, H. L. (1999) · The Journal of Infectious Diseases · doi:10.1086/315033 · Cited by 466
Self-citationRasmussen, K. R., Arrowood, M. J., & Healey, M. C. (1992) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.36.1.220 · Cited by 33
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Metadata
- Authors
- Arrowood, M. J., & Sterling, C. R.
- Venue
- The Journal of Parasitology
- Publisher
- JSTOR
- Published
- 1987-04
- Volume
- 73
- Issue
- 2
- Pages
- 314
- ISSN
- 0022-3395 (ISSN-L)
- OpenAlex
- W2019402469
- Cited by
- 419
- References
- 0
- FWCI
- 4.39
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: Isolation of Cryptosporidium Oocysts and Sporozoites Using Discontinuous Sucrose and Isopycnic Percoll Gradients. https://app.cochat.ai/papers/10.2307/3282084
- The paper
- Arrowood, M. J., & Sterling, C. R. (1987). Isolation of Cryptosporidium Oocysts and Sporozoites Using Discontinuous Sucrose and Isopycnic Percoll Gradients. The Journal of Parasitology. https://doi.org/10.2307/3282084
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
