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Cited by 134Toxoplasma gondii Research StudiesCoccidia and coccidiosis researchParasitic infections in humans and animals

Long-Term Antibody Responses of Cats Fed Toxoplasma gondii Tissue Cysts

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Dubey, J. P., Lappin, M. R., Thulliez, P.·1995·The Journal of Parasitology
DOI 10.2307/3284035
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Abstract

As part of a long-term study on immunity to oocyst shedding, 12 4-6-mo-old cats were inoculated orally with tissue cysts of the ME-49 strain (6 cats) or the TS-2 strain (6 cats) of Toxoplasma gondii. Two cats fed the ME-49 strain died or were killed because of acute toxoplasmosis 12 and 13 days after inoculation (DAI), respectively. On day 39 after primary infection, 5 cats (2 infected with the ME-49 strain and 3 infected with the TS-2 strain) were challenged orally with tissue cysts of the ME-49 strain. One cat died following rechallenge infection due to causes unrelated to toxoplasmosis. Seventy-seven months after primary infection, the remaining 9 cats were challenged orally with tissue cysts of the P89 strain of T. gondii. Blood samples were obtained weekly or monthly and sera were analyzed for antibodies to T. gondii using the modified agglutination test (MAT), the Sabin-Feldman dye test (DT), and the enzyme-linked immunosorbent assay (ELISA) for IgM (IgM-ELISA) or IgG (IgG-ELISA). The MAT was performed using both formalin-fixed (FF) and acetone-fixed (AF) tachyzoites. The MAT (FF) was the most sensitive test; cats seroconverted within 14 DAI and high titers (> 10,000) persisted > 6 yr, although cats had no clinical signs. The MAT titers using the AF detected recent exposure and titers declined sharply after 2 mo postinoculation. DT and ELISA titers were lower and developed slower than MAT titers. Fluctuations in antibody titers were limited to 8-fold during the 6-yr observation period. Anamnestic serum antibody responses were seen in 2 cats after the final challenge, but not after first challenge.

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Authors
Dubey, J. P., Lappin, M. R., & Thulliez, P.
Affiliations
Agricultural Research Service (US)
ORCID
Venue
The Journal of Parasitology
Publisher
JSTOR
Published
1995-12
Volume
81
Issue
6
Pages
887
ISSN
0022-3395 (ISSN-L)
DOI
10.2307/3284035
OpenAlex
W1976388001
Cited by
134
References
27
Citations by year
2026: 1 · 2025: 3 · 2022: 7 · 2021: 7 · 2020: 9 · 2019: 3 · 2018: 3 · 2017: 6 · 2016: 4 · 2015: 8 · 2014: 4 · 2013: 4 · 2012: 3
FWCI
1.78
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Journal of Parasitology (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Parasitology › Toxoplasma gondii Research Studies
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Coccidia and coccidiosis research
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Parasitic infections in humans and animals
Keywords
Toxoplasma gondii, cats, antibody response, tissue cysts, oral infection, ME49 strain, modified agglutination test, Sabin-Feldman dye test, secondary immune response, long-term immunity, toxoplasmosis, seroconversion
MeSH terms
Agglutination Tests, Animals, Antibodies, Protozoan, Aqueous Humor, Cat Diseases, Cats, Disease Vectors, Enzyme-Linked Immunosorbent Assay, Feces, Female, Male, Parasite Egg Count, Toxoplasma, Toxoplasmosis, Animal

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