Effect of Weight Reduction on in Vitro Adipose Tissue Lipolysis and Cellularity in Obese Adolescents and Adults
Abstract
Adipose tissue cellularity, in vitro glycerol release, and in vivo glucose metabolism were examined in ten markedly obese subjects, ages thirteen to fifty-four, before and after weight reduction. Prior to reduction adipose cells were larger and exhibited higher rates of lipolysis than smaller postreduction cells. Incubations containing epinephrine, epinephrine plus insulin and insulin alone also revealed significant decreases in absolute rates of glycerol release in the postreduction state. However, when the data were expressed as per cent change, in vitro hormonal activity did not appear to be influenced by changes in cell size. Weight reduction was also accompanied by improvement in oral glucose tolerance tests, lower fasting levels of plasma free fatty acids and decreased values of circulating immunoreactive insulin, fasting and following oral glucose. Studies of adipose cell number revealed that obese adolescents display hypercellularity comparable to that observed in obese adults. As in the adult, weight loss resulted in improved glucose tolerance and a decrease in adipose cell size without a significant change in total adipose cell number. Thus decreased rates of lipolysis of smaller adipocytes were associated with the improved lipid and glucose metabolism found in obese subjects after weight reduction.
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Weight loss decreases fat-cell size.
“Weight loss decreases visceral fat mass, 26 intramyocellular fat, 27 intrahepatic fat, 28 fat-cell size, 29 and the rate of release of fatty acids from adipose tissue.30”
From Absence of an Effect of Liposuction on Insulin Action and Risk Factors for Coronary Heart Disease (2004) · cites this paper as [29]
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- Authors
- Knittle, J. L., & Ginsberg-Fellner, F.
- Affiliations
- City University of New York (US)
- Division of Nutrition and Metabolism, Department of Pediatrics, Mount Sinai School of Medicine of the City University of New York New York, N.Y. 10029
- Venue
- Diabetes
- Publisher
- American Diabetes Association
- Published
- 1972-06-01
- Volume
- 21
- Issue
- 6
- Pages
- 754-761
- ISSN
- 0012-1797 (ISSN-L)
- 1939-327X
- OpenAlex
- W2166441029
- Cited by
- 65
- References
- 0
- Citations by year
- 2016: 1
- FWCI
- 9.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Diabetes (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Health Research Council of the City of New York
- Topics
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- weight loss, obesity, adipose tissue lipolysis, adipocyte size, adipocyte number, glycerol release, glucose tolerance, insulin, epinephrine, free fatty acids, insulin resistance, adolescents, adults
- MeSH terms
- Adipose Tissue, Adolescent, Adult, Blood Glucose, Body Height, Body Water, Body Weight, Cell Count, Diet, Reducing, Epinephrine, Fatty Acids, Nonesterified, Female, Glucose Tolerance Test, Glycerol, Humans, Insulin, Male, Middle Aged, Obesity, Tritium
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