Cited by 60Metabolism, Diabetes, and CancerMitochondrial Function and PathologyAdipose Tissue and Metabolism

Insulin-regulated mitochondrial gene expression is associated with glucose flux in human skeletal muscle.

Huang, X, Eriksson, K F, Vaag, A, Lehtovirta, M, Hansson, M, Laurila, E, Kanninen, T, Olesen, B T +3 more·1999·Diabetes

Abstract

To identify abnormally expressed genes contributing to muscle insulin resistance in type 2 diabetes, we screened the mRNA populations from normal and diabetic human skeletal muscle using cDNA differential display and isolated abnormally expressed cDNA clones of mitochondrial-encoded NADH dehydrogenase 1 (ND1), cytochrome oxidase 1, tRNA(leu), and displacement loop. We then measured mRNA expression of these mitochondrial genes using a relative quantitative polymerase chain reaction method in biopsies taken before and after an insulin clamp in 12 monozygotic twin pairs discordant for type 2 diabetes and 12 matched control subjects and in muscle biopsies taken after an insulin clamp from 13 subjects with type 2 diabetes, 15 subjects with impaired glucose tolerance, and 14 subjects with normal glucose tolerance. Insulin infusion increased mRNA expression of ND1 from 1.02 +/- 0.04 to 2.55 +/- 0.30 relative units (P < 0.001) and of cytochrome oxidase 1 from 0.80 +/- 0.01 to 1.24 +/- 0.10 relative units (P < 0.001). The ND1 response to insulin correlated with glucose uptake (r = 0.46, P = 0.002). Although the rate of insulin-mediated glucose uptake was decreased in the diabetic versus the nondiabetic twins (5.2 +/- 0.7 vs. 8.5 +/- 0.8 mg x kg(-1) fat-free mass x min(-1), P < 0.01), insulin-stimulated ND1 expression was not significantly different between them (2.4 +/- 0.5 vs. 2.7 +/- 0.5 relative units). Neither was there any significant intrapair correlation of ND1 expression between the monozygotic twins (r = -0.15, NS). We conclude that insulin upregulates mitochondrial-encoded gene expression in skeletal muscle. Given the positive correlation between ND1 expression and glucose uptake and the lack of intrapair correlation between monozygotic twins, mitochondrial gene expression may represent an adaptation to intracellular glucose flux rather than an inherited trait.

Integrity signals

Registry

Crossref · 10.2337/diabetes.48.8.1508

Cites

15 of 15 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Huang, X., Eriksson, K. F., Vaag, A., Lehtovirta, M., Hansson, M., Laurila, E., Kanninen, T., Olesen, B. T., Kurucz, I., Koranyi, L., & Groop, L.
Affiliations
Lund University (SE)
Venue
Diabetes
Published
1999-08-01
Volume
48
Issue
8
Pages
1508-1514
ISSN
  • 0012-1797 (ISSN-L)
  • 1939-327X
OpenAlex
W2151584333
Cited by
60
References
15
Citations by year
2020: 1 · 2019: 1 · 2016: 1 · 2015: 2 · 2014: 1 · 2013: 1 · 2012: 4
FWCI
1.03
Type
journal article
Language
en
Open access
closed
Locations
  • Diabetes (publishedVersion)
  • PubMed (publishedVersion)
  • RUCforsk (Roskilde University) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
Keywords
insulin regulation, mitochondrial gene expression, skeletal muscle, type 2 diabetes, insulin resistance, glucose uptake, cytochrome c oxidase subunit I, monozygotic twins, impaired glucose tolerance, glucose flux
MeSH terms
Aged, Diabetes Mellitus, Type 2, Diseases in Twins, Female, Gene Expression Regulation, Glucose, Humans, Insulin, Insulin Resistance, Male, Middle Aged, Mitochondria, Reference Values, Twins, Monozygotic, DNA, Complementary, Muscle, Skeletal, Reverse Transcriptase Polymerase Chain Reaction

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review