The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Impairment of Insulin Signaling in Human Skeletal Muscle Cells by Co-Culture With Human Adipocytes
Open accessAbstract
Adipocyte factors play a major role in the induction of insulin resistance in skeletal muscle. To analyze this cross-talk, we established a system of co-culture of human fat and skeletal muscle cells. Cells of three muscle donors were kept in co-culture with cells of various fat cell donors, and insulin signaling was subsequently analyzed in myocytes. Insulin-induced tyrosine phosphorylation of insulin receptor substrate (IRS)-1 was completely blocked, with unaltered expression of IRS-1. Troglitazone increased insulin action on IRS-1 phosphorylation, in both the absence and presence of co-culture. Insulin-regulated activation of Akt kinase in the myocytes was significantly reduced after co-culture, with troglitazone restoring insulin action. Addition of tumor necrosis factor (TNF)-α (2.5 nmol/l) to myocytes for 48 h reduced IRS-1 expression and inhibited IRS-1 and Akt phosphorylation comparable to the effect of co-culture. Lower doses of TNF-α were ineffective. After co-culture, TNF-α in the culture medium was below the detection limit of 0.3 pmol/l. A very low level of resistin was detected in the supernatant of myocytes, but not of adipocytes. In conclusion, the release of fat cell factors induces insulin resistance in human skeletal muscle cells; however, TNF-α and resistin appear not to be involved in this process.
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Mechanism by which metformin reduces glucose production in type 2 diabetes.
Hundal, R. S., Krssak, M., Dufour, S., Laurent, D., Lebon, V, Chandramouli, V, Inzucchi, S. E., Schumann, W. C., Petersen, K. F., Landau, B. R., et al. (2000) · Diabetes · doi:10.2337/diabetes.49.12.2063 · Cited by 1,052
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“Furthermore, adipose tissue is also dispersed within muscle (15).”
BackgroundKim, Y.-B., Nikoulina, S. E., Ciaraldi, T. P., Henry, R. R., & Kahn, B. B. (1999) · Journal of Clinical Investigation · doi:10.1172/jci6928 · Cited by 487
Fat content in individual muscle fibers of lean and obese subjects.
Malenfant, P., Joanisse, D., Thériault, R., Goodpaster, B., Kelley, D., & Simoneau, J.-A. (2001) · International Journal of Obesity · doi:10.1038/sj.ijo.0801733 · Cited by 227
Skeletal muscle and insulin sensitivity: pathophysiological alterations
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Cited by
Adipokines: inflammation and the pleiotropic role of white adipose tissue
Trayhurn, P., & Wood, I. S. (2004) · British Journal of Nutrition · doi:10.1079/bjn20041213 · Cited by 2,210
Endocrine and signalling role of adipose tissue: new perspectives on fat
Trayhurn, P. (2005) · Acta Physiologica Scandinavica · doi:10.1111/j.1365-201x.2005.01468.x · Cited by 502
Resistin messenger-RNA expression is increased by proinflammatory cytokines in vitro
Kaser, S., Kaser, A., Sandhofer, A., Ebenbichler, C. F., Tilg, H., & Patsch, J. R. (2003) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2003.07.003 · Cited by 462
Human adipocytes secrete mineralocorticoid-releasing factors
Ehrhart-Bornstein, M., Lamounier-Zepter, V., Schraven, A., Langenbach, J., Willenberg, H. S., Barthel, A., Hauner, H., McCann, S. M., Scherbaum, W. A., & Bornstein, S. R. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2336140100 · Cited by 443
Self-citationSecretory factors from human adipose tissue and their functional role
Hauner, H. (2005) · Proceedings of the Nutrition Society · doi:10.1079/pns2005428 · Cited by 391
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Metadata
- Authors
- Dietze, D., Koenen, M., Röhrig, K., Horikoshi, H., Hauner, H., & Eckel, J.
- Venue
- Diabetes
- Publisher
- American Diabetes Association
- Published
- 2002-08-01
- Volume
- 51
- Issue
- 8
- Pages
- 2369-2376
- ISSN
- 0012-1797 (ISSN-L)
- 1939-327X
- OpenAlex
- W2100098822
- Cited by
- 183
- References
- 7
- FWCI
- 7.84
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Dietze, D., Koenen, M., Röhrig, K., Horikoshi, H., Hauner, H., & Eckel, J. (2002). Impairment of Insulin Signaling in Human Skeletal Muscle Cells by Co-Culture With Human Adipocytes. Diabetes. https://doi.org/10.2337/diabetes.51.8.2369
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