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Cited by 229Muscle Physiology and DisordersAdipose Tissue and MetabolismDiabetes, Cardiovascular Risks, and Lipoproteins

Fat content in individual muscle fibers of lean and obese subjects.

Malenfant, P, Joanisse, DR, Thériault, R, Goodpaster, BH, Kelley, DE, Simoneau, J-A·2001·International Journal of Obesity
DOI 10.1038/sj.ijo.0801733
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Abstract

Objective To examine skeletal muscle intracellular triglyceride concentration in different fiber types in relation to obesity.Design Skeletal muscle fiber type distribution and intracellular lipid content were measured in vastus lateralis samples obtained by needle biopsy from lean and obese individuals.Subjects Seven lean controls (body mass index (BMI) 23.0+/-3.3 kg/m(2); mean+/-s.d.) and 14 obese (BMI 33.7+/-2.7 kg/m(2)) individuals; both groups included comparable proportions of men and women.Measurements Samples were histochemically stained for the identification of muscle fiber types (myosin ATPase) and intracellular lipid aggregates (oil red O dye). The number and size of fat aggregates as well as their concentration within type I, IIA and IIB muscle fiber types were measured. The cellular distribution of the lipid aggregates was also examined.Results The size of fat aggregates was not affected by obesity but the number of lipid droplets within muscle fibers was twice as abundant in obese compared to lean individuals. This was seen in type I (298+/-135 vs 129+/-75; obese vs lean, P<0.05), IIA (132+/-67 vs 79+/-29; P<0.05), and IIB (103+/-63 vs 51+/-13; P<0.05) muscle fibers. A more central distribution of lipid droplets was observed in muscle fibers of obese compared to lean subjects (27.2+/-5.7 vs 19.7+/-6.4%; P<0.05).Conclusion The higher number of lipid aggregates and the disposition to a greater central distribution in all fiber types in obesity indicate important changes in lipid metabolism and/or storage that are fiber type-independent.

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  • Intramyocellular lipid excess strongly correlates with the severity of insulin resistance, even after correction for the degree of obesity, and has been observed in muscles of multiple fiber types.

    “Although it remains unclear whether any of these defects play a causal role in insulin resistance, intramyocellular lipid excess strongly correlates with the severity of insulin resistance, even after correction for the degree of obesity (94), and has been observed in muscles of multiple fiber types (95).”

    From The role of mitochondria in the pathogenesis of type 2 diabetes (2010) · cites this paper as [95]

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Authors
Malenfant, P., Joanisse, D., Thériault, R., Goodpaster, B., Kelley, D., & Simoneau, J.-A.
Affiliations
  • Université Laval (CA)
  • University of Pittsburgh (US)
Corresponding author
Kelley, DE
ORCID
Thériault, R
Venue
International Journal of Obesity
Publisher
Springer Science and Business Media LLC
Published
2001-09
Volume
25
Issue
9
Pages
1316-1321
ISSN
  • 0307-0565 (ISSN-L)
  • 1476-5497
DOI
10.1038/sj.ijo.0801733
OpenAlex
W2089177804
Cited by
227
References
31
Citations by year
2026: 1 · 2025: 8 · 2024: 8 · 2023: 4 · 2022: 9 · 2021: 8 · 2020: 11 · 2019: 9 · 2018: 8 · 2017: 9 · 2016: 8 · 2015: 10 · 2014: 10 · 2013: 8 · 2012: 16
FWCI
2.91
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • International Journal of Obesity (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health
  • National Institute of Diabetes and Digestive and Kidney Diseases (R01DK049200, R01 DK49200-03)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes, Cardiovascular Risks, and Lipoproteins
Keywords
obesity, skeletal muscle, intracellular triglycerides, lipid droplets, muscle fiber type, vastus lateralis, lipid metabolism, fat deposition, myosin ATPase histochemistry, Oil Red O staining
MeSH terms
Adult, Azo Compounds, Biopsy, Needle, Coloring Agents, Female, Histocytochemistry, Humans, Male, Myosins, Obesity, Triglycerides, Muscle, Skeletal, Muscle Fibers, Skeletal

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