The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Involvement of AMP-Activated Protein Kinase in Glucose Uptake Stimulated by the Globular Domain of Adiponectin in Primary Rat Adipocytes
Open accessAbstract
Adiponectin is an abundant adipocyte-derived plasma protein with anti-atherosclerotic and insulin-sensitizing properties that suppresses hepatic glucose production and enhances glucose uptake into skeletal muscle. To characterize the potential effects of adiponectin on glucose uptake into adipose cells, we incubated isolated epididymal rat adipocytes with the globular domain of recombinant adiponectin purified from an E. coli expression system. Globular adiponectin increased glucose uptake in adipocytes without stimulating tyrosine phosphorylation of the insulin receptor or insulin receptor substrate-1, and without enhancing phosphorylation of Akt on Ser-473. Globular adiponectin further enhanced insulin-stimulated glucose uptake at submaximal insulin concentrations and reversed the inhibitory effect of tumor necrosis factor-α on insulin-stimulated glucose uptake. Cellular treatment with globular adiponectin increased the Thr-172 phosphorylation and catalytic activity of AMP-activated protein kinase and enhanced the Ser-79 phosphorylation of acetyl CoA carboxylase, an enzyme downstream of AMP kinase in adipose cells. Inhibition of AMP kinase activation using two pharmacological inhibitors (adenine 9-β-d-arabinofuranoside and compound C) completely abrogated the increase in glucose uptake stimulated by globular adiponectin, indicating that AMP kinase is integrally involved in the adiponectin signal transduction pathway. Coupled with recent evidence that the effects of adiponectin are mediated via AMP kinase activation in liver and skeletal muscle, the findings reported here provide an important mechanistic link in the signaling effects of adiponectin in diverse metabolically responsive tissues.
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Cites
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Mammalian AMP-activated protein kinase subfamily
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Cited by
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Type 2 diabetes: principles of pathogenesis and therapy
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Self-citationAdiponectin and Adiponectin Receptors
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Metadata
- Authors
- Wu, X., Motoshima, H., Mahadev, K., Stalker, T. J., Scalia, R., & Goldstein, B. J.
- Venue
- Diabetes
- Publisher
- American Diabetes Association
- Published
- 2003-06-01
- Volume
- 52
- Issue
- 6
- Pages
- 1355-1363
- ISSN
- 0012-1797 (ISSN-L)
- 1939-327X
- OpenAlex
- W2122328486
- Cited by
- 463
- References
- 12
- FWCI
- 23.99
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Wu, X., Motoshima, H., Mahadev, K., Stalker, T. J., Scalia, R., & Goldstein, B. J. (2003). Involvement of AMP-Activated Protein Kinase in Glucose Uptake Stimulated by the Globular Domain of Adiponectin in Primary Rat Adipocytes. Diabetes. https://doi.org/10.2337/diabetes.52.6.1355
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