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Cited by 477

The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.

Identification of Coronavirus Isolated from a Patient in Korea with COVID-19

Open access
Kim, Jeong-Min, Chung, Yoon-Seok, Jo, Hye Jun, Lee, Nam-Joo, Kim, Mi Seon, Woo, Sang Hee, Park, Sehee, Kim, Jee Woong, Kim, Heui Man, Han, Myung-Guk·2020·Osong Public Health and Research Perspectives

Abstract

OBJECTIVES: Following reports of patients with unexplained pneumonia at the end of December 2019 in Wuhan, China, the causative agent was identified as coronavirus (SARS-CoV-2), and the 2019 novel coronavirus disease was named COVID-19 by the World Health Organization. Putative patients with COVID-19 have been identified in South Korea, and attempts have been made to isolate the pathogen from these patients. METHODS: Upper and lower respiratory tract secretion samples from putative patients with COVID-19 were inoculated onto cells to isolate the virus. Full genome sequencing and electron microscopy were used to identify the virus. RESULTS: The virus replicated in Vero cells and cytopathic effects were observed. Full genome sequencing showed that the virus genome exhibited sequence homology of more than 99.9% with SARS-CoV-2 which was isolated from patients from other countries, for instance China. Sequence homology of SARS-CoV-2 with SARS-CoV, and MERS-CoV was 77.5% and 50%, respectively. Coronavirus-specific morphology was observed by electron microscopy in virus-infected Vero cells. CONCLUSION: SARS-CoV-2 was isolated from putative patients with unexplained pneumonia and intermittent coughing and fever. The isolated virus was named BetaCoV/Korea/KCDC03/2020.

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Metadata

Authors
Kim, J.-M., Chung, Y.-S., Jo, H. J., Lee, N.-J., Kim, M. S., Woo, S. H., Park, S., Kim, J. W., Kim, H. M., & Han, M.-G.
Venue
Osong Public Health and Research Perspectives
Published
2020-02-28
Volume
11
Issue
1
Pages
3-7
ISSN
  • 2210-9099 (ISSN-L)
  • 2233-6052
OpenAlex
W3007223608
Cited by
477
References
14
FWCI
12.98
Type
journal article
Language
en
Open access
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CoChat. (2026). Citation check: Identification of Coronavirus Isolated from a Patient in Korea with COVID-19. https://app.cochat.ai/papers/10.24171/j.phrp.2020.11.1.02
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Kim, J.-M., Chung, Y.-S., Jo, H. J., Lee, N.-J., Kim, M. S., Woo, S. H., Park, S., Kim, J. W., Kim, H. M., & Han, M.-G. (2020). Identification of Coronavirus Isolated from a Patient in Korea with COVID-19. Osong Public Health and Research Perspectives. https://doi.org/10.24171/j.phrp.2020.11.1.02

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