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Cited by 18Animal health and immunologyVeterinary Medicine and SurgeryAnimal Virus Infections Studies

Evaluation of surrogate markers for passive transfer of immunity in kittens

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Crawford, P. Cynda, Levy, Julie K., Werner, Linda L.·2006·Journal of the American Veterinary Medical Association
DOI 10.2460/javma.228.7.1038
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Abstract

Objective—To identify surrogate markers of passive transfer of immunity in kittens. Design—Prospective clinical trial. Animals—55 kittens from 12 specific-pathogen–free queens. Procedure—Kittens were allocated at birth into colostrum-fed (n = 27) and colostrum-deprived (28) groups. Blood was collected at birth and on days 1, 2, 4, 7, 14, 28, and 56. Serum samples were analyzed for activities of alkaline phosphatase (ALP), alanine aminotransferase, aspartate aminotransferase, creatine kinase, lactate dehydrogenase, γ-glutamyltransferase, amylase, and lipase and for concentrations of albumin, total protein, bilirubin, urea nitrogen, creatinine, cholesterol, glucose, calcium, phosphorus, and triglycerides by use of an automated analyzer. Total serum solids concentrations were estimated by use of refractometry. Serum IgG concentrations were quantified by use of radial immunodiffusion. Results—All kittens were agammaglobulinemic at birth. Colostrum-fed kittens had significantly higher IgG concentrations than did colostrum-deprived kittens from 1 though 28 days of age. Transient significant differences in serum biochemical variables between the colostrum-deprived and colostrum-fed groups were substantially resolved by day 4. Passive transfer of immunity could be reliably determined at 1 day of age and to a lesser extent at 2 days of age only by measurement of serum activity of ALP. Conclusions and Clinical Relevance—Adequacy of passive transfer in kittens initially correlated with serum activity of ALP, but quantification of serum IgG concentration was necessary after 2 days of age.

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Crossref · 10.2460/javma.228.7.1038

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  • Colostrum, produced during the first 2 days after parturition, represents the main source of immunoglobulins and nutrients for the newborn kitten, and also contributes to digestive tract maturation.

    “Colostrum, produced during the first 2 days after parturition, represents the main source of immunoglobulins and nutrients for the newborn kitten, and also contributes to digestive tract maturation.”

    From Feline neonatology: From birth to commencement of weaning – what to know for successful management (2022) · cites this paper as [29]

    The Results report that kittens had no detectable IgG before nursing and that colostrum-fed kittens had higher serum IgG than colostrum-deprived kittens. The Results also identify nutrients in colostrum. The copy does not establish that colostrum is produced specifically during the first two days, that it is the main source of nutrients, or that it contributes to digestive tract maturation (searched for those terms); page numbers were not available. (LLM, conf=0.86)

    Experimental — accuracy not yet measured

  • Adequate passive immune transfer can be indirectly assessed by measuring alkaline phosphatase activity in the blood, with a threshold of 1500 IU/ml at day 1.

    “Adequate passive immune transfer can also be indirectly assessed by measuring alkaline phosphatase activity in the blood, with a threshold of 1500 iU/ml at day 1.”

    From Feline neonatology: From birth to commencement of weaning – what to know for successful management (2022) · cites this paper as [29]

    The Results report that serum alkaline phosphatase (ALP) activity distinguished colostrum-fed from colostrum-deprived kittens on day 1, using a breakpoint of 1,500 U/L. The citing sentence gives the threshold as 1,500 IU/mL, which is 1,000 times higher than 1,500 U/L. (LLM, conf=0.93)

    Experimental — accuracy not yet measured

  • All kittens were found to be agammaglobulinaemic at birth.

    “in a study by Crawford et al, all kittens were found to be agammaglobulinaemic at birth”

    From Feline neonatology: From birth to commencement of weaning – what to know for successful management (2022) · cites this paper as [29]

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Cites

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3 of the 29 references couldn’t be matched to a known paper.

Research works printed but not linked: 2

  1. Kolb E. Constituents of the colostrum and milk of queens and their significance: a review. Tierarztl Umsch 2004;59:589-594.
  2. McMichael M, Dhupa N. Pediatric critical care medicine: specific syndromes. Compend Contin Educ Pract Vet 2000;22: 353-359.

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Authors
Crawford, P. C., Levy, J. K., & Werner, L. L.
Affiliations
University of Florida (US)
ORCID
Venue
Journal of the American Veterinary Medical Association
Publisher
American Veterinary Medical Association (AVMA)
Published
2006-04-01
Volume
228
Issue
7
Pages
1038-1041
ISSN
0003-1488 (ISSN-L)
DOI
10.2460/javma.228.7.1038
OpenAlex
W2028737536
Cited by
18
References
29
Citations by year
2025: 1 · 2024: 1 · 2023: 1 · 2022: 1 · 2015: 1 · 2014: 1 · 2013: 2 · 2012: 3
FWCI
2.88
Type
journal article
Language
en
Open access
Locations
  • Journal of the American Veterinary Medical Association (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Veterinary › Small Animals › Animal health and immunology
  • Health Sciences › Veterinary › Small Animals › Veterinary Medicine and Surgery
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
Keywords
passive transfer, kittens, colostrum feeding, colostrum deprivation, serum alkaline phosphatase, serum IgG, surrogate markers, neonatal kittens, agammaglobulinemia, serum biochemistry
MeSH terms
Aging, Alanine Transaminase, Alkaline Phosphatase, Animals, Animals, Newborn, Animals, Suckling, Aspartate Aminotransferases, Blood Chemical Analysis, Cats, Colostrum, Creatine Kinase, Female, gamma-Glutamyltransferase, Immunoglobulin G, Immunity, Maternally-Acquired, L-Lactate Dehydrogenase, Male, Prospective Studies, Specific Pathogen-Free Organisms

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