Cited by 933RNA Interference and Gene DeliveryNanoparticle-Based Drug DeliveryDendrimers and Hyperbranched Polymers

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The Proton Sponge: a Trick to Enter Cells the Viruses Did Not Exploit

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Behr, Jean-Paul·1997·CHIMIA

DOI 10.2533/chimia.1997.34

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Abstract

Several non-permanent polycations possessing substantial buffering capacity below physiological pH, such as lipopolyamines and polyethylenimines, are efficient transfection agents per se, i.e. without the addition of lysosomotropic bases, or cell targeting, or membrane disruption agents. These vectors have been shown to deliver genes as well as oligonucleotides both in vitro and in vivo. Our hypothesis is that their efficiency relies on extensive endosome swelling and rupture that provides an escape mechanism for the polycation/DNA particles.

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“Due to the existence of plenty of electropositive amino groups, PEI often has excellent ability to deliver gene sequences into cells with the help of the proton sponge mechanism (Behr, 1997).”
Background
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Authors: Behr, J.-P.
Affiliations: Centre National de la Recherche Scientifique (FR)
Corresponding author: Behr, Jean-Paul
Venue: CHIMIA
Publisher: Swiss Chemical Society
Published: 1997-02-26
Volume: 51
Issue: 1-2
Pages: 34
ISSN: 0009-4293 (ISSN-L)
2673-2424
DOI: 10.2533/chimia.1997.34
OpenAlex: W4292328694
Cited by: 933
References: 1
Citations by year: 2026: 18 · 2025: 31 · 2024: 30 · 2023: 34 · 2022: 24 · 2021: 41 · 2020: 56 · 2019: 47 · 2018: 50 · 2017: 38 · 2016: 58 · 2015: 50 · 2014: 55 · 2013: 78 · 2012: 49
FWCI: 1.07
Type: journal article
Language: en
Open access: diamond
doi.org
Licence: cc-by-nc
creativecommons.org/licenses/by-nc/4.0/ (unspecified)
Locations: CHIMIA International Journal for Chemistry (publishedVersion, cc-by-nc)
DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
Physical Sciences › Materials Science › Polymers and Plastics › Dendrimers and Hyperbranched Polymers
Keywords: proton sponge, polyethyleneimine, endosomal escape, gene delivery, oligonucleotide delivery, buffering capacity

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The Proton Sponge: a Trick to Enter Cells the Viruses Did Not Exploit

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