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Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections
Open accessAbstract
We present a rigorously validated and highly sensitive confirmatory real-time RT-PCR assay (1A assay) that can be used in combination with the previously reported upE assay. Two additional RT-PCR assays for sequencing are described, targeting the RdRp gene (RdRpSeq assay) and N gene (NSeq assay), where an insertion/deletion polymorphism might exist among different hCoV-EMC strains. Finally, a simplified and biologically safe protocol for detection of antibody response by immunofluorescence microscopy was developed using convalescent patient serum.
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Cited by
Virological assessment of hospitalized patients with COVID-2019
Wölfel, R., Corman, V. M., Guggemos, W., Seilmaier, M., Zange, S., Müller, M. A., Niemeyer, D., Jones, T. C., Vollmar, P., Rothe, C., et al. (2020) · Nature · doi:10.1038/s41586-020-2196-x · Cited by 6,661
Self-citationAssiri, A., Al-Tawfiq, J. A., Al-Rabeeah, A. A., Al-Rabiah, F. A., Al-Hajjar, S., Al-Barrak, A., Flemban, H., Al-Nassir, W. N., Balkhy, H. H., Al-Hakeem, R. F., et al. (2013) · The Lancet Infectious Diseases · doi:10.1016/s1473-3099(13)70204-4 · Cited by 1,435
SARS-CoV-2-reactive T cells in healthy donors and patients with COVID-19
Braun, J., Loyal, L., Frentsch, M., Wendisch, D., Georg, P., Kurth, F., Hippenstiel, S., Dingeldey, M., Kruse, B., Fauchere, F., et al. (2020) · Nature · doi:10.1038/s41586-020-2598-9 · Cited by 1,310
Self-citationMiddle East Respiratory Syndrome
Arabi, Y. M., Balkhy, H. H., Hayden, F. G., Bouchama, A., Luke, T., Baillie, J. K., Al-Omari, A., Hajeer, A. H., Senga, M., Denison, M. R., et al. (2017) · New England Journal of Medicine · doi:10.1056/nejmsr1408795 · Cited by 518
Saad, M., Omrani, A. S., Baig, K., Bahloul, A., Elzein, F., Matin, M. A., Selim, M. A. A., Mutairi, M. A., Nakhli, D. A., Aidaroos, A. Y. A., et al. (2014) · International Journal of Infectious Diseases · doi:10.1016/j.ijid.2014.09.003 · Cited by 486
Metadata
- Authors
- Corman, V. M., Müller, M. A., Costabel, U., Timm, J., Binger, T., Meyer, B., Kreher, P., Lattwein, E., Eschbach-Bludau, M., Nitsche, A., Bleicker, T., Landt, O., Schweiger, B., Drexler, J. F., Osterhaus, A. D., Haagmans, B. L., Dittmer, U., Bonin, F., Wolff, T., & Drosten, C.
- Venue
- Eurosurveillance
- Published
- 2012-12-06
- Volume
- 17
- Issue
- 49
- ISSN
- 1025-496X (ISSN-L)
- 1560-7917
- OpenAlex
- W1852588318
- Cited by
- 360
- References
- 0
- FWCI
- 25.29
- Type
- journal article
- Language
- en
- Open access
- diamond
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- The paper
- Corman, V. M., Müller, M. A., Costabel, U., Timm, J., Binger, T., Meyer, B., Kreher, P., Lattwein, E., Eschbach-Bludau, M., Nitsche, A., Bleicker, T., Landt, O., Schweiger, B., Drexler, J. F., Osterhaus, A. D., Haagmans, B. L., Dittmer, U., Bonin, F., Wolff, T., & Drosten, C. (2012). Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections. Eurosurveillance. https://doi.org/10.2807/ese.17.49.20334-en
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
