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Cited by 10Pneumonia and Respiratory InfectionsBacterial Infections and VaccinesBacterial Identification and Susceptibility Testing

The DOI matches its OpenAlex record; 31 of the 31 references it cites were found in a registry or an index.

Molecular Detection and Serotyping of Streptococcus pneumoniae in Children with Suspected Meningitis in Northeast Iran.

Open access
Sepideh Abdoli, Saghar Safamanesh, Mahsa Khosrojerdi, Amir Azimian·2020·PubMed Central
DOI 10.30476/ijms.2019.45423PMC PMC7071549
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Abstract

BACKGROUND: in developing countries, serotyping of the prevalent isolates is useful in selecting the correct vaccine. The present study aimed to evaluate common serotypes of pneumococcal meningitis in Bojnurd, Iran. METHODS: . In addition, the modified Marimon's PCR method was used for serotyping the bacteria. The data were analyzed using Pearson's Chi-square test. RESULTS: using the PCR method, while only 92 cases tested positive using the conventional methods. Based on the Marimon's PCR method, serotypes 23F, 19F, 19A, 1, 14, and serogroup 6A/B were the most common types. Serogroups 18C, 15A/F, 15B/C, 9A/V, 7A/F, 11A/D/F, and 22A/F were also detected in isolates. Note that 2.8% of the samples were non-typable (NT). CONCLUSION: International Congress of Microbiology, as a poster and published in the congress abstracts book.

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Figure 1 Agarose gel electrophoresis of triplex PCR on standard samples. The 268 bp, 187 bp, and 114 bp bands belong to beta-globin, lytA, psaA genes, respectively. Lanes, 1: 100 bp ladder; 2: 107 copy; 3: 106 copy; 4: 105 copy; 5: 104 copy; 6: 103 copy; 7: 102 copy; 8: 101 copy of S. pneumoniae genome
Figure 1 Agarose gel electrophoresis of triplex PCR on standard samples. The 268 bp, 187 bp, and 114 bp bands belong to beta-globin, lytA, psaA genes, respectively. Lanes, 1: 100 bp ladder; 2: 107 copy; 3: 106 copy; 4: 105 copy; 5: 104 copy; 6: 103 copy; 7: 102 copy; 8: 101 copy of S. pneumoniae genomeCC BY-ND · PubMed Central
Figure 2 Serotype distribution among S. pneumoniae strains.
Figure 2 Serotype distribution among S. pneumoniae strains.CC BY-ND · PubMed Central

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Metadata

Authors
Abdoli, S., Safamanesh, S., Khosrojerdi, M., & Azimian, A.
Affiliations
  • North Khorasan University of Medical Sciences (IR)
  • Semnan University of Medical Sciences (IR)
ORCID
Amir Azimian
Venue
PubMed Central
Publisher
National Institutes of Health
Published
2020-03-01
Volume
45
Issue
2
Pages
125-133
DOI
10.30476/ijms.2019.45423
PMC
PMC7071549
OpenAlex
W2972288738
Cited by
10
References
30
Citations by year
2025: 1 · 2024: 4 · 2023: 2 · 2022: 1 · 2021: 2
FWCI
0.90
Type
journal article
Language
en
Open access
Licence
cc-by-nd
Locations
  • PubMed Central (publishedVersion, cc-by-nd)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Health Sciences › Medicine › Epidemiology › Pneumonia and Respiratory Infections
  • Life Sciences › Immunology and Microbiology › Microbiology › Bacterial Infections and Vaccines
  • Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
Keywords
Streptococcus pneumoniae, pneumococcal meningitis, serotyping, serogroups, molecular detection, vaccine selection, children, Bojnurd

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