Cited by 10Pneumonia and Respiratory InfectionsBacterial Infections and VaccinesBacterial Identification and Susceptibility Testing

The DOI matches its OpenAlex record; 31 of the 31 references it cites were found in a registry or an index.

Molecular Detection and Serotyping of Streptococcus pneumoniae in Children with Suspected Meningitis in Northeast Iran.

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Abstract

BACKGROUND: in developing countries, serotyping of the prevalent isolates is useful in selecting the correct vaccine. The present study aimed to evaluate common serotypes of pneumococcal meningitis in Bojnurd, Iran. METHODS: . In addition, the modified Marimon's PCR method was used for serotyping the bacteria. The data were analyzed using Pearson's Chi-square test. RESULTS: using the PCR method, while only 92 cases tested positive using the conventional methods. Based on the Marimon's PCR method, serotypes 23F, 19F, 19A, 1, 14, and serogroup 6A/B were the most common types. Serogroups 18C, 15A/F, 15B/C, 9A/V, 7A/F, 11A/D/F, and 22A/F were also detected in isolates. Note that 2.8% of the samples were non-typable (NT). CONCLUSION: International Congress of Microbiology, as a poster and published in the congress abstracts book.

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OpenAlex · 10.30476/ijms.2019.45423

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Figures

Figure 1 Agarose gel electrophoresis of triplex PCR on standard samples. The 268 bp, 187 bp, and 114 bp bands belong to beta-globin, lytA, psaA genes, respectively. Lanes, 1: 100 bp ladder; 2: 107 copy; 3: 106 copy; 4: 105 copy; 5: 104 copy; 6: 103 copy; 7: 102 copy; 8: 101 copy of S. pneumoniae genome
Figure 1 Agarose gel electrophoresis of triplex PCR on standard samples. The 268 bp, 187 bp, and 114 bp bands belong to beta-globin, lytA, psaA genes, respectively. Lanes, 1: 100 bp ladder; 2: 107 copy; 3: 106 copy; 4: 105 copy; 5: 104 copy; 6: 103 copy; 7: 102 copy; 8: 101 copy of S. pneumoniae genomeCC BY-ND · PubMed Central
Figure 2 Serotype distribution among S. pneumoniae strains.
Figure 2 Serotype distribution among S. pneumoniae strains.CC BY-ND · PubMed Central

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Metadata

Authors
Abdoli, S., Safamanesh, S., Khosrojerdi, M., & Azimian, A.
Affiliations
  • North Khorasan University of Medical Sciences (IR)
  • Semnan University of Medical Sciences (IR)
Venue
PubMed Central
Publisher
National Institutes of Health
Published
2020-03-01
Volume
45
Issue
2
Pages
125-133
OpenAlex
W2972288738
Cited by
10
References
30
Citations by year
2025: 1 · 2024: 4 · 2023: 2 · 2022: 1 · 2021: 2
FWCI
0.90
Type
journal article
Language
en
Open access
Licence
cc-by-nd
Locations
  • PubMed Central (publishedVersion, cc-by-nd)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Health Sciences › Medicine › Epidemiology › Pneumonia and Respiratory Infections
  • Life Sciences › Immunology and Microbiology › Microbiology › Bacterial Infections and Vaccines
  • Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
Keywords
Streptococcus pneumoniae, pneumococcal meningitis, serotyping, serogroups, molecular detection, vaccine selection, children, Bojnurd

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