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Electroimmuno Assay
Abstract
Quantitative analysis of proteins by electrophoresis into antibody containing agarose gel - the electroimmuno assay - is described with special reference to technical details and to the variation in appearance of the immunopreci-pitates in different antigen-antibody systems. The utility of the method is compared with the single radial immunodiffusion technique. Both methods have similar sensitivity and precision. They are simple enough for clinical routine work. The results are obtained faster and differences in size of the antigen-carrying molecules in samples are often easier to recognise with the electrophoretic technique, which also facilitates evaluation of separations obtained on protein chromatography.
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Citations
Cited by
Hyperinsulinemia as an independent risk factor for ischemic heart disease
Després, J.-P., Lamarche, B., Mauriège, P., Cantin, B., Dagenais, G. R., Moorjani, S., & Lupien, P.-J. (1996) · New England Journal of Medicine · doi:10.1056/nejm199604113341504 · Cited by 1,699
Owen, M. C., Brennan, S. O., Lewis, J. H., & Carrell, R. W. (1983) · New England Journal of Medicine · doi:10.1056/nejm198309223091203 · Cited by 426
Garg, A., Bonanome, A., Grundy, S. M., Zhang, Z.-J., & Unger, R. H. (1988) · New England Journal of Medicine · doi:10.1056/nejm198809293191304 · Cited by 394
Apolipoproteins in human cerebrospinal fluid.
Roheim, P. S., Carey, M., Forte, T., & Vega, G. L. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.9.4646 · Cited by 282
Albumin homeostasis in patients undergoing continuous ambulatory peritoneal dialysis
Kaysen, G. A., & Schoenfeld, P. Y. (1984) · Kidney International · doi:10.1038/ki.1984.15 · Cited by 193
Metadata
- Authors
- Laurell, C.-B.
- Venue
- Scandinavian Journal of Clinical and Laboratory Investigation
- Publisher
- Informa UK Limited
- Published
- 1972-01
- Volume
- 29
- Issue
- sup124
- Pages
- 21-37
- ISSN
- 0036-5513 (ISSN-L)
- 1502-7686
- OpenAlex
- W4251824554
- Cited by
- 1,687
- References
- 0
- FWCI
- 16.40
- Type
- journal article
- Language
- en
- Open access
- closed
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- Laurell, C.-B. (1972). Electroimmuno Assay. Scandinavian Journal of Clinical and Laboratory Investigation. https://doi.org/10.3109/00365517209102748
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
