Cited by 15Gastrointestinal motility and disordersPain Mechanisms and TreatmentsVagus Nerve Stimulation Research

Plasticity of dorsal root ganglion neurons in a rat model of post-infectious gut dysfunction: potential implication of nerve growth factor

Jardí, Ferran, Fernández-Blanco, Joan A., Martínez, Vicente, Vergara, Patri·2014·Scandinavian Journal of Gastroenterology

Abstract

OBJECTIVE: Intestinal infections are suggested as a risk factor for the development of irritable bowel syndrome (IBS)-like visceral hypersensitivity. The mechanisms implicated might involve long-term changes in visceral afferents, with implication of nerve growth factor (NGF). We explored plastic changes in dorsal root ganglia (DRGs) receiving innervation from the gut and the potential implication of NGF in a rat model of IBS-like post-infectious gut dysfunction. MATERIALS AND METHODS: Rats were infected with Trichinella spiralis larvae. Thirty days post-infection, inflammatory markers, including interleukins (ILs) and mucosal mast cell infiltration (rat mast cell protease II [RMCPII]), and NGF and TrkA expression was determined in the jejunum and colon (RT-qPCR). In the same animals, morphometry (neuronal body size) and NGF content (immunofluorescence) were assessed in thoracolumbar DRG neurons. RESULTS: In infected animals, a low-grade inflammatory-like response, characterized by up-regulated levels of RMCPII and IL-6, was observed in the jejunum and colon. TrkA expression was increased in the jejunum, whereas the colon showed a slight reduction. NGF levels remained unaltered regardless the gut region. Overall, the mean cross-sectional area of DRG neurons was increased in T. spiralis-infected animals, with a reduction in both TrkA and NGF staining. CONCLUSIONS: Results suggest that during T. spiralis infection in rats, there is a remodeling of sensory afferents that might imply a NGF-mediated mechanism. Plastic changes in sensory afferents might mediate the long-lasting functional alterations that characterize this model of IBS. Similar mechanisms might be operating in patients with post-infectious-IBS.

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Crossref · 10.3109/00365521.2014.958524

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Authors
Jardí, F., Fernández-Blanco, J. A., Martínez, V., & Vergara, P.
Affiliations
  • Universitat Autònoma de Barcelona (ES)
  • Department of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, Barcelona, Spain
Venue
Scandinavian Journal of Gastroenterology
Published
2014-09-26
Volume
49
Issue
11
Pages
1296-1303
ISSN
  • 0036-5521 (ISSN-L)
  • 1502-7708
OpenAlex
W2082586056
Cited by
15
References
32
Citations by year
2026: 1 · 2025: 1 · 2022: 1 · 2019: 2 · 2018: 2 · 2016: 4 · 2015: 3 · 2014: 1
FWCI
2.67
Type
journal article
Language
en
Open access
closed
Locations
  • Scandinavian Journal of Gastroenterology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Generalitat de Catalunya
Topics
  • Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
  • Health Sciences › Medicine › Physiology › Pain Mechanisms and Treatments
  • Life Sciences › Neuroscience › Neurology › Vagus Nerve Stimulation Research
Keywords
dorsal root ganglion neurons, nerve growth factor, TrkA, Trichinella spiralis infection, post-infectious irritable bowel syndrome, visceral hypersensitivity, intestinal inflammation, mast cells, interleukin-6, intestinal infection
MeSH terms
Animals, Colitis, Disease Models, Animal, Ganglia, Spinal, Male, Neuronal Plasticity, Rats, Sprague-Dawley, Nerve Growth Factor, Irritable Bowel Syndrome, Rats

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