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A Direct Spectrophotometric Microassay for Sulfated Glycosaminoglycans in Cartilage Cultures
Abstract
A rapid spectrophotometric procedure is described for the estimation of sulfated glycosaminoglycans in cartilage cultures. Papain digestion of tissue or culture medium provides glycosaminoglycans in solution for assay; an aliquot of the digest is mixed with the dye 1,9-dimethylmethylene blue. The assay is based on the metachromatic shift in absorption maximum which occurs when the dye is complexed with sulfated glycosaminoglycans. The reagent is stable, and the method is substantially free from interference, is sensitive to less than 1 microgram (4 micrograms/ml) of chondroitin sulfate, and provides a simple alternative to the traditional methods for glycosaminoglycan determinations.
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Citations
Cited by
Barry, F., Boynton, R. E., Liu, B., & Murphy, J. M. (2001) · Experimental Cell Research · doi:10.1006/excr.2001.5278 · Cited by 1,020
ADAMTS5 is the major aggrecanase in mouse cartilage in vivo and in vitro
Stanton, H., Rogerson, F. M., East, C. J., Golub, S. B., Lawlor, K. E., Meeker, C. T., Little, C. B., Last, K., Farmer, P. J., Campbell, I. K., et al. (2005) · Nature · doi:10.1038/nature03417 · Cited by 915
Mouser, V. H. M., Melchels, F. P. W., Visser, J., Dhert, W. J. A., Gawlitta, D., & Malda, J. (2016) · Biofabrication · doi:10.1088/1758-5090/8/3/035003 · Cited by 359
Synergistic Action of Growth Factors and Dynamic Loading for Articular Cartilage Tissue Engineering
Mauck, R. L., Nicoll, S. B., Seyhan, S. L., Ateshian, G. A., & Hung, C. T. (2003) · Tissue Engineering · doi:10.1089/107632703768247304 · Cited by 337
Morales, T. I., & Roberts, A. B. (1988) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)37634-8 · Cited by 274
Metadata
- Authors
- Farndale, R. W., Sayers, C. A., & Barrett, A. J.
- Venue
- Connective Tissue Research
- Publisher
- Informa UK Limited
- Published
- 1982-01
- Volume
- 9
- Issue
- 4
- Pages
- 247-248
- ISSN
- 0300-8207 (ISSN-L)
- 1607-8438
- OpenAlex
- W2057635936
- Cited by
- 1,347
- References
- 0
- FWCI
- 5.27
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: A Direct Spectrophotometric Microassay for Sulfated Glycosaminoglycans in Cartilage Cultures. https://app.cochat.ai/papers/10.3109/03008208209160269
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- Farndale, R. W., Sayers, C. A., & Barrett, A. J. (1982). A Direct Spectrophotometric Microassay for Sulfated Glycosaminoglycans in Cartilage Cultures. Connective Tissue Research. https://doi.org/10.3109/03008208209160269
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
