The DOI matches its Crossref record; 60 of the 60 references it cites were found in a registry or an index.
Yield stress determines bioprintability of hydrogels based on gelatin-methacryloyl and gellan gum for cartilage bioprinting
Open accessAbstract
Bioprinting of chondrocyte-laden hydrogels facilitates the fabrication of constructs with controlled organization and shape e.g. for articular cartilage implants. Gelatin-methacryloyl (gelMA) supplemented with gellan gum is a promising bio-ink. However, the rheological properties governing the printing process, and the influence of gellan gum on the mechanical properties and chondrogenesis of the blend, are still unknown. Here, we investigated the suitability of gelMA/gellan for cartilage bioprinting. Multiple concentrations, ranging from 3% to 20% gelMA with 0%-1.5% gellan gum, were evaluated for their printability, defined as the ability to form filaments and to incorporate cells at 15 °C-37 °C. To support the printability assessment, yield stress and viscosity of the hydrogels were measured. Stiffness of UV-cured constructs, as well as cartilage-like tissue formation by embedded chondrocytes, were determined in vitro. A large range of gelMA/gellan concentrations were printable with inclusion of cells and formed the bioprinting window. The addition of gellan gum improved filament deposition by inducing yielding behavior, increased construct stiffness and supported chondrogenesis. High gellan gum concentrations, however, did compromise cartilage matrix production and distribution, and even higher concentrations resulted in too high yield stresses to allow cell encapsulation. This study demonstrates the high potential of gelMA/gellan blends for cartilage bioprinting and identifies yield stress as a dominant factor for bioprintability.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
3D bioprinting of tissues and organs
Murphy, S. V., & Atala, A. (2014) · Nature Biotechnology · doi:10.1038/nbt.2958 · Cited by 7,034
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
Discher, D. E., Janmey, P., & Wang, Y.-L. (2005) · Science · doi:10.1126/science.1116995 · Cited by 6,382
Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels
Yue, K., Trujillo-de Santiago, G., Alvarez, M. M., Tamayol, A., Annabi, N., & Khademhosseini, A. (2015) · Biomaterials · doi:10.1016/j.biomaterials.2015.08.045 · Cited by 3,146
Cell-laden microengineered gelatin methacrylate hydrogels
Nichol, J. W., Koshy, S. T., Bae, H., Hwang, C. M., Yamanlar, S., & Khademhosseini, A. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.03.064 · Cited by 2,377
Designing Cell-Compatible Hydrogels for Biomedical Applications
Seliktar, D. (2012) · Science · doi:10.1126/science.1214804 · Cited by 1,923
25th Anniversary Article: Engineering Hydrogels for Biofabrication
Malda, J., Visser, J., Melchels, F. P., Jüngst, T., Hennink, W. E., Dhert, W. J. A., Groll, J., & Hutmacher, D. W. (2013) · Advanced Materials · doi:10.1002/adma.201302042 · Cited by 1,901
Self-citationCurrent advances and future perspectives in extrusion-based bioprinting
Ozbolat, I. T., & Hospodiuk, M. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2015.10.076 · Cited by 1,572
Extracellular-matrix tethering regulates stem-cell fate
Trappmann, B., Gautrot, J. E., Connelly, J. T., Strange, D. G. T., Li, Y., Oyen, M. L., Cohen Stuart, M. A., Boehm, H., Li, B., Vogel, V., et al. (2012) · Nature Materials · doi:10.1038/nmat3339 · Cited by 1,568
Structural and Rheological Properties of Methacrylamide Modified Gelatin Hydrogels
Van Den Bulcke, A. I., Bogdanov, B., De Rooze, N., Schacht, E. H., Cornelissen, M., & Berghmans, H. (2000) · Biomacromolecules · doi:10.1021/bm990017d · Cited by 1,536
A Direct Spectrophotometric Microassay for Sulfated Glycosaminoglycans in Cartilage Cultures
Farndale, R. W., Sayers, C. A., & Barrett, A. J. (1982) · Connective Tissue Research · doi:10.3109/03008208209160269 · Cited by 1,347
Cited by
Printability and shape fidelity of bioinks in 3D bioprinting
Schwab, A., Levato, R., D’Este, M., Piluso, S., Eglin, D., & Malda, J. (2020) · Chemical Reviews · doi:10.1021/acs.chemrev.0c00084 · Cited by 1,359
Self-citationCell-laden hydrogels for osteochondral and cartilage tissue engineering
Yang, J., Zhang, Y. S., Yue, K., & Khademhosseini, A. (2017) · Acta Biomaterialia · doi:10.1016/j.actbio.2017.01.036 · Cited by 652
Hydrogel Bioink Reinforcement for Additive Manufacturing: A Focused Review of Emerging Strategies
Chimene, D., Kaunas, R., & Gaharwar, A. K. (2020) · Advanced Materials · doi:10.1002/adma.201902026 · Cited by 600
Assessment methodologies for extrusion-based bioink printability
Gillispie, G., Prim, P., Copus, J., Fisher, J., Mikos, A. G., Yoo, J. J., Atala, A., & Lee, S. J. (2020) · Biofabrication · doi:10.1088/1758-5090/ab6f0d · Cited by 426
Recent Advances in Photo-Crosslinkable Hydrogels for Biomedical Applications
Choi, J. R., Yong, K. W., Choi, J. Y., & Cowie, A. C. (2019) · BioTechniques · doi:10.2144/btn-2018-0083 · Cited by 403
Metadata
- Authors
- Mouser, V. H. M., Melchels, F. P. W., Visser, J., Dhert, W. J. A., Gawlitta, D., & Malda, J.
- Venue
- Biofabrication
- Publisher
- IOP Publishing
- Published
- 2016-07-19
- Volume
- 8
- Issue
- 3
- Pages
- 035003
- ISSN
- 1758-5082 (ISSN-L)
- 1758-5090
- OpenAlex
- W2500442904
- Cited by
- 359
- References
- 60
- FWCI
- 12.62
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Yield stress determines bioprintability of hydrogels based on gelatin-methacryloyl and gellan gum for cartilage bioprinting. https://app.cochat.ai/papers/10.1088/1758-5090/8/3/035003
- The paper
- Mouser, V. H. M., Melchels, F. P. W., Visser, J., Dhert, W. J. A., Gawlitta, D., & Malda, J. (2016). Yield stress determines bioprintability of hydrogels based on gelatin-methacryloyl and gellan gum for cartilage bioprinting. Biofabrication. https://doi.org/10.1088/1758-5090/8/3/035003
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
