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Restriction Endonuclease
Abstract
This review provides a comprehensive account of the current status of the biology and biochemistry of restriction endonucleases. Both Class I and Class II restriction endonucleases will be considered. However, emphasis will be placed on the Class II group, which recognizes and cleaves a specific duplex DNA sequence. Their occurrence, purification, and characterization is discussed in detail. The characterization includes physical mapping information and determination of recognition sequences. In addition to detailed discussions of the biochemical properties of the enzymes, considerable attention is paid to the uses of these enzymes as tools for research in molecular biology. These uses include physical mapping of genomes and their transcripts, genetic analysis (marker rescue, etc.), DNA sequence analysis, analysis of complex genomes, and genetic engineering. Specific examples of each use are outlined. Practical aspects of both the isolation and use of the restriction endonucleases form the major theme of this review.
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Citations
Cites
A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase
Sanger, F., & Coulson, A. R. (1975) · Journal of Molecular Biology · doi:10.1016/0022-2836(75)90213-2 · Cited by 2,709
Cited by
Rapid evolution of animal mitochondrial DNA
Brown, W. M., George, M., & Wilson, A. C. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.4.1967 · Cited by 3,439
DNA Methylation and Gene Function
Razin, A., & Riggs, A. D. (1980) · Science · doi:10.1126/science.6254144 · Cited by 2,071
Rat Insulin Genes: Construction of Plasmids Containing the Coding Sequences
Ullrich, A., Shine, J., Chirgwin, J., Pictet, R., Tischer, E., Rutter, W. J., & Goodman, H. M. (1977) · Science · doi:10.1126/science.325648 · Cited by 1,238
Use of restriction enzymes to study eukaryotic DNA methylation
Bird, A. P., & Southern, E. M. (1978) · Journal of Molecular Biology · doi:10.1016/0022-2836(78)90242-5 · Cited by 443
Novel bacteriophage lambda cloning vector.
Karn, J., Brenner, S., Barnett, L., & Cesareni, G. (1980) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.77.9.5172 · Cited by 386
Metadata
- Authors
- Roberts, R. J., & Murray, K.
- Venue
- CRC Critical Reviews in Biochemistry
- Publisher
- Informa UK Limited
- Published
- 1976-01
- Volume
- 4
- Issue
- 2
- Pages
- 123-164
- ISSN
- 0045-6411 (ISSN-L)
- OpenAlex
- W4210974853
- Cited by
- 411
- References
- 1
- FWCI
- 15.18
- Type
- journal article
- Language
- en
- Open access
- closed
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- Roberts, R. J., & Murray, K. (1976). Restriction Endonuclease. CRC Critical Reviews in Biochemistry. https://doi.org/10.3109/10409237609105456
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.