The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Novel bacteriophage lambda cloning vector.
Open accessAbstract
A simple method for generating phage collections representing eukaryotic genomes has been developed by using a novel bacteriophage lambda vector, lambda 1059. The phage is a BamHI substitution vector that accommodates DNA fragments 6-24 kilobases long. Production of recombinants in lambda 1059 requires deletion of the lambda red and gamma genes. The recombinants are therefore spi- and may be separated from the spi+ vector phages by plating on strains lysogenic for bacteriophage P2. Random fragments suitable for insertion into lambda 1059 are obtained by partial digestion of high molecular weight eukaryotic DNA with Sau3a. This restriction enzyme cleaves at the sequence G-A-T-C and leaves a 5'-tetranucleotide "sticky end." Because G-A-T-C extensions are also produced by BamHI cleavage, these fragments may be annealed directly to BamHI-cleaved lambda 1059. By using these methods, a set of clones covering the entire Caenorhabditis elegans genome was constructed. DNA segments which include the unc-54 myosin heavy chain gene have been isolated from this collection.
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Characterization of the human factor VIII gene
Gitschier, J., Wood, W. I., Goralka, T. M., Wion, K. L., Chen, E. Y., Eaton, D. H., Vehar, G. A., Capon, D. J., & Lawn, R. M. (1984) · Nature · doi:10.1038/312326a0 · Cited by 988
Nucleotide polymorphism at the alcohol dehydrogenase locus of Drosophila melanogaster
Kreitman, M. (1983) · Nature · doi:10.1038/304412a0 · Cited by 708
Spi-1 is a putative oncogene in virally induced murine erythroleukaemias
Moreau-Gachelin, F., Tavitian, A., & Tambourin, P. (1988) · Nature · doi:10.1038/331277a0 · Cited by 447
The basal component of the nematode dense-body is vinculin
Barstead, R. J., & Waterston, R. H. (1989) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)81782-3 · Cited by 253
de Vos, W. M., Vos, P., de Haard, H., & Boerrigter, I. (1989) · Gene · doi:10.1016/0378-1119(89)90477-0 · Cited by 121
Metadata
- Authors
- Karn, J., Brenner, S., Barnett, L., & Cesareni, G.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1980-09
- Volume
- 77
- Issue
- 9
- Pages
- 5172-5176
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2088400936
- Cited by
- 386
- References
- 23
- FWCI
- 43.99
- Type
- journal article
- Language
- en
- Open access
- green
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- Karn, J., Brenner, S., Barnett, L., & Cesareni, G. (1980). Novel bacteriophage lambda cloning vector. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.77.9.5172
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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