Incorporation of PVMMA to PLGA MS enhances lectin grafting and their in vitro activity in macrophages
Abstract
Attachment of lectins, especially those that are recognized and bounded by macrophages and other antigen presenting cells onto biodegradable microspheres surface, may serve as support for a wide variety of applications. The aim of this work was: (1) to prepare microspheres (MS) based on two biodegradable copolymers, poly (lactide-co-glicolide) acid (PLGA) and poly[(methyl vinyl ether)-co-(maleic anhydride)] (PVMMA) with their surface functionalized with Concanavalin A (Con A) and, (2) to evaluate their behaviour in macrophage culture. The incorporation of PVMMA was studied in order to augment the lectin coupling efficiency. MS were obtained by spray-drying and bovine serum albumin (BSA) was loaded as protein model. Particles were characterized for size, morphology, surface charge, entrapment efficiency, FTIR, in vitro protein release and conjugation efficiency. Finally, functionalized MS were tested in vitro with raw 264.7 murine macrophages (Mф) in terms of cytotoxicity, phagocytosis, nitric oxide (NO) production, and oxygen consumption. Conjugated Con A microspheres showed increased grafting efficiency up to four times compared to PLGA alone. The retention of Con A after coupling was confirmed by desorption studies. The attachment of Con A to microspheres induced oxygen consumption, increased phagocytosis efficiency and even NO production by macrophages. The results suggest that Con A and possibly, other lectins, grafted onto PLGA-PVMMA microspheres may serve as potential adjuvants by modulating protein delivery and macrophage activation.
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Concanavalin A interacts with intestinal epithelium and immune cells like macrophages, activating them and upregulating TLRs and production of NO and proinflammatory cytokines.
“Concanavalin A can not only interact with the intestinal epithelium but also with immune cells, such as macrophages, resulting in their activation and subsequent upregulation of TLRs as well as the production of NO and proinflammatory cytokines [110–112].”
From Use of lectin-functionalized particles for oral immunotherapy (2012) · cites this paper as [110]
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Use of lectin-functionalized particles for oral immunotherapy
Diesner, S. C., Wang, X.-Y., Jensen-Jarolim, E., Untersmayr, E., & Gabor, F. (2012) · Therapeutic Delivery · doi:10.4155/tde.11.146 · Cited by 40 · see citing works
- Authors
- León-Rodriguez, L., Leiro-Vidal, J., Blanco-Méndez, J., & Luzardo-Álvarez, A.
- Affiliations
- Universidade de Santiago de Compostela (ES)
- ORCID
- Blanco-Méndez, José
- Venue
- International Journal of Pharmaceutics
- Publisher
- Elsevier BV
- Published
- 2010-12-15
- Volume
- 402
- Issue
- 1-2
- Pages
- 165-174
- ISSN
- 0378-5173 (ISSN-L)
- OpenAlex
- W1976986602
- Cited by
- 11
- References
- 48
- Citations by year
- 2018: 1 · 2016: 2 · 2015: 1 · 2013: 2 · 2012: 4
- FWCI
- 1.56
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Journal of Pharmaceutics (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Xunta de Galicia
- European Social Fund
- Topics
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Keywords
- PLGA microspheres, concanavalin A, macrophages, phagocytosis, nitric oxide production, oxygen consumption, biodegradable microspheres, spray drying, bovine serum albumin, protein delivery, adjuvants, macrophage activation, cytotoxicity
- MeSH terms
- Animals, Cattle, Cell Line, Concanavalin A, Drug Carriers, Macrophages, Maleates, Microspheres, Nitric Oxide, Oxygen Consumption, Phagocytosis, Polyethylenes, Polyglactin 910, Serum Albumin, Bovine, Lectins, Mice
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