Determination of new biomarkers to monitor the dietary consumption of isothiocyanates
Abstract
Isothiocyanates (ITCs) found in cruciferous vegetables have been associated with a reduced cancer risk in humans. We determined serum albumin adducts of allyl isothiocyanate (AITC), benzylisothiocyanate (BITC), phenylethylisothiocyanate (PEITC) and sulforaphane (SFN) in 85 healthy men from a dietary, randomized, controlled trial. After enzymatic digestion of albumin we determined the adducts of the ITCs with lysine (Lys) using liquid chromatography-tandem mass spectrometry. At the beginning of the study (and after 4 weeks) 4.7% (2.4%), 48.2% (35.3%), 5.9% (10.6%), and 24.7% (23.5%) of the samples were found positive for AITC-Lys, BITC-Lys, PEITC-Lys and SFN-Lys, respectively. This method enables the quantification of ITC adducts in albumin from large, prospective studies on diet and cancer.
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Isothiocyanates and acrylamide form adducts with blood albumin and hemoglobin, with half-lives of 3 to 8 weeks, and may be used as longer-term biomarkers.
“Some dietary compounds such as isothiocyanates and acrylamide also form adducts with blood albumin and hemoglobin (37,38), with half-lives varying between 3 and 8 wk, and may be used as longer-term biomarkers.”
From The food metabolome: a window over dietary exposure (2014) · cites this paper as [37]
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- Authors
- Kumar, A., Vineis, P., Sacerdote, C., Fiorini, L., & Sabbioni, G.
- Affiliations
- Tulane University (US)
- Imperial College London (GB)
- University of Turin (IT)
- Corresponding author
- Sabbioni, Gabriele
- Venue
- Biomarkers
- Publisher
- Informa UK Limited
- Published
- 2010-11-02
- Volume
- 15
- Issue
- 8
- Pages
- 739-745
- ISSN
- 1354-750X (ISSN-L)
- 1366-5804
- OpenAlex
- W2083528864
- Cited by
- 19
- References
- 31
- Citations by year
- 2024: 2 · 2023: 1 · 2022: 1 · 2019: 1 · 2018: 2 · 2016: 1 · 2014: 3 · 2013: 3 · 2012: 1
- FWCI
- 1.04
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biomarkers (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics, phytochemicals, and oxidative stress
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Nutritional Studies and Diet
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Keywords
- isothiocyanates, dietary biomarkers, lysine adducts, allyl isothiocyanate, benzyl isothiocyanate, phenylethyl isothiocyanate, sulforaphane, cruciferous vegetables, chemoprevention, dietary exposure assessment
- MeSH terms
- Adult, Aged, Chromatography, Liquid, Diet, Humans, Male, Middle Aged, Biomarkers, Isothiocyanates, Tandem Mass Spectrometry
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