Cited by 19Genomics, phytochemicals, and oxidative stressNutritional Studies and DietCarcinogens and Genotoxicity Assessment

Determination of new biomarkers to monitor the dietary consumption of isothiocyanates

Kumar, Anoop, Vineis, Paolo, Sacerdote, Carlotta, Fiorini, Laura, Sabbioni, Gabriele·2010·Biomarkers

Abstract

Isothiocyanates (ITCs) found in cruciferous vegetables have been associated with a reduced cancer risk in humans. We determined serum albumin adducts of allyl isothiocyanate (AITC), benzylisothiocyanate (BITC), phenylethylisothiocyanate (PEITC) and sulforaphane (SFN) in 85 healthy men from a dietary, randomized, controlled trial. After enzymatic digestion of albumin we determined the adducts of the ITCs with lysine (Lys) using liquid chromatography-tandem mass spectrometry. At the beginning of the study (and after 4 weeks) 4.7% (2.4%), 48.2% (35.3%), 5.9% (10.6%), and 24.7% (23.5%) of the samples were found positive for AITC-Lys, BITC-Lys, PEITC-Lys and SFN-Lys, respectively. This method enables the quantification of ITC adducts in albumin from large, prospective studies on diet and cancer.

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Crossref · 10.3109/1354750x.2010.517567

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  • Isothiocyanates and acrylamide form adducts with blood albumin and hemoglobin, with half-lives of 3 to 8 weeks, and may be used as longer-term biomarkers.

    “Some dietary compounds such as isothiocyanates and acrylamide also form adducts with blood albumin and hemoglobin (37,38), with half-lives varying between 3 and 8 wk, and may be used as longer-term biomarkers.”

    From The food metabolome: a window over dietary exposure (2014) · cites this paper as [37]

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Authors
Kumar, A., Vineis, P., Sacerdote, C., Fiorini, L., & Sabbioni, G.
Affiliations
  • Tulane University (US)
  • Imperial College London (GB)
  • University of Turin (IT)
Corresponding author
Sabbioni, Gabriele
Venue
Biomarkers
Published
2010-11-02
Volume
15
Issue
8
Pages
739-745
ISSN
  • 1354-750X (ISSN-L)
  • 1366-5804
OpenAlex
W2083528864
Cited by
19
References
31
Citations by year
2024: 2 · 2023: 1 · 2022: 1 · 2019: 1 · 2018: 2 · 2016: 1 · 2014: 3 · 2013: 3 · 2012: 1
FWCI
1.04
Type
journal article
Language
en
Open access
closed
Locations
  • Biomarkers (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics, phytochemicals, and oxidative stress
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Nutritional Studies and Diet
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
Keywords
isothiocyanates, dietary biomarkers, lysine adducts, allyl isothiocyanate, benzyl isothiocyanate, phenylethyl isothiocyanate, sulforaphane, cruciferous vegetables, chemoprevention, dietary exposure assessment
MeSH terms
Adult, Aged, Chromatography, Liquid, Diet, Humans, Male, Middle Aged, Biomarkers, Isothiocyanates, Tandem Mass Spectrometry

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