Cited by 58Heavy Metal Exposure and ToxicityEpigenetics and DNA MethylationCarcinogens and Genotoxicity Assessment

p16 promoter methylation in Pb2+-exposed individuals

Kovatsi, Leda, Georgiou, Elisavet, Ioannou, Antrea, Haitoglou, Costas, Tzimagiorgis, George, Tsoukali, Helen, Kouidou, Sofia·2010·Clinical Toxicology

Abstract

BACKGROUND: One of the principle symptoms of lead poisoning is the development of neurological disorders. Neuronal response is closely related to DNA methylation changes. Aim. In this study, we estimated p16 methylation in nine individuals exposed to lead using methylation-specific polymerase chain reaction followed by analysis of the methylated cytosine content of the product by thermal denaturation. RESULTS: We found that, based on lead blood concentration, lead-exposed individuals were divided into two groups. Among highly exposed individuals (blood Pb(2+) concentration = 51-100 microg/dL), we observed complete CpG methylation, whereas for low Pb(2+) concentrations (blood Pb(2+) concentration = 6-11 microg/dL), we observed partial methylation. CONCLUSION: Our results show that among lead-overexposed individuals, p16 methylation is frequent and extensive, and suggest that DNA methylation could be involved in the mechanism by which lead induces neurotoxicity.

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Crossref · 10.3109/15563650903567091

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Authors
Kovatsi, L., Georgiou, E., Ioannou, A., Haitoglou, C., Tzimagiorgis, G., Tsoukali, H., & Kouidou, S.
Affiliations
Aristotle University of Thessaloniki (GR)
Corresponding author
Kovatsi, Leda
Venue
Clinical Toxicology
Published
2010-02
Volume
48
Issue
2
Pages
124-128
ISSN
  • 0009-9309 (ISSN-L)
  • 1556-3650
  • 1556-9519
OpenAlex
W2014744184
Cited by
58
References
24
Citations by year
2026: 3 · 2025: 3 · 2024: 3 · 2023: 5 · 2022: 3 · 2021: 7 · 2020: 1 · 2019: 3 · 2018: 3 · 2017: 4 · 2016: 4 · 2015: 6 · 2014: 4 · 2013: 3 · 2012: 3
FWCI
2.20
Type
journal article
Language
en
Open access
closed
Locations
  • Clinical Toxicology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Heavy Metal Exposure and Toxicity
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
Keywords
p16, DNA methylation, lead exposure, lead poisoning, neurotoxicity, blood lead levels, CpG methylation, methylated cytosine, methylation-specific PCR, thermal denaturation
MeSH terms
Adult, Cytosine, Humans, Lead, Male, Middle Aged, Promoter Regions, Genetic, Case-Control Studies, Polymerase Chain Reaction, Occupational Exposure, CpG Islands, DNA Methylation, Genes, p16, Lead Poisoning, Nervous System, Adult

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