p16 promoter methylation in Pb2+-exposed individuals
Abstract
BACKGROUND: One of the principle symptoms of lead poisoning is the development of neurological disorders. Neuronal response is closely related to DNA methylation changes. Aim. In this study, we estimated p16 methylation in nine individuals exposed to lead using methylation-specific polymerase chain reaction followed by analysis of the methylated cytosine content of the product by thermal denaturation. RESULTS: We found that, based on lead blood concentration, lead-exposed individuals were divided into two groups. Among highly exposed individuals (blood Pb(2+) concentration = 51-100 microg/dL), we observed complete CpG methylation, whereas for low Pb(2+) concentrations (blood Pb(2+) concentration = 6-11 microg/dL), we observed partial methylation. CONCLUSION: Our results show that among lead-overexposed individuals, p16 methylation is frequent and extensive, and suggest that DNA methylation could be involved in the mechanism by which lead induces neurotoxicity.
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Men with lower lead levels had partial to no DNA methylation of the p16 gene.
“In contrast, men with lower lead levels had partial to no DNA methylation of the p16 gene [ 46 ].”
From DNA methylation: a mechanism linking environmental chemical exposures to risk of autism spectrum disorders? (2016) · cites this paper as [46]
Epidemiological studies suggest that lead can induce changes in DNA methylation.
“Epidemiological studies also suggest that lead can induce changes in DNA methylation [ 44 , 46 , 124 ].”
From DNA methylation: a mechanism linking environmental chemical exposures to risk of autism spectrum disorders? (2016) · cites this paper as [46]
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- Authors
- Kovatsi, L., Georgiou, E., Ioannou, A., Haitoglou, C., Tzimagiorgis, G., Tsoukali, H., & Kouidou, S.
- Affiliations
- Aristotle University of Thessaloniki (GR)
- Corresponding author
- Kovatsi, Leda
- Venue
- Clinical Toxicology
- Publisher
- Informa UK Limited
- Published
- 2010-02
- Volume
- 48
- Issue
- 2
- Pages
- 124-128
- ISSN
- 0009-9309 (ISSN-L)
- 1556-3650
- 1556-9519
- OpenAlex
- W2014744184
- Cited by
- 58
- References
- 24
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 3 · 2023: 5 · 2022: 3 · 2021: 7 · 2020: 1 · 2019: 3 · 2018: 3 · 2017: 4 · 2016: 4 · 2015: 6 · 2014: 4 · 2013: 3 · 2012: 3
- FWCI
- 2.20
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Clinical Toxicology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Heavy Metal Exposure and Toxicity
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Keywords
- p16, DNA methylation, lead exposure, lead poisoning, neurotoxicity, blood lead levels, CpG methylation, methylated cytosine, methylation-specific PCR, thermal denaturation
- MeSH terms
- Adult, Cytosine, Humans, Lead, Male, Middle Aged, Promoter Regions, Genetic, Case-Control Studies, Polymerase Chain Reaction, Occupational Exposure, CpG Islands, DNA Methylation, Genes, p16, Lead Poisoning, Nervous System, Adult
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