Cited by 151Proteins in Food SystemsRuminant Nutrition and Digestive PhysiologyAnimal Diversity and Health Studies

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Structural changes of bovine milk fat globules during in vitro digestion

Open access
Gallier, S., Ye, A., Singh, H.·2012·Journal of Dairy Science

Abstract

An in vitro digestion model that simulated gastric and intestinal fasting conditions was used to monitor the physical, chemical, and structural changes of fat globules from raw bovine milk. During in vitro gastric digestion, the fat globules were stable under low-acidic conditions. Some peptides and β-lactoglobulin were resistant to proteolysis by pepsin. Phospholipids, proteins, and peptides stabilized the globules in the stomach model. During in vitro intestinal digestion, most of the β-lactoglobulin and residual peptides were hydrolyzed by trypsin and chymotrypsin, and the lipolytic products, released from the hydrolysis of the triglyceride core of the globules, led to destabilization and coalescence of the globules. By accumulating at the surface of the fat globules, the lipolytic products formed a lamellar phase and their solubilization by bile salts resulted in the formation of disk-shaped micelles. This study brings new interesting insights on the digestion of bovine milk.

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Metadata

Authors
Gallier, S., Ye, A., & Singh, H.
Affiliations
Riddet Institute (NZ)
Corresponding author
Gallier, S.
Venue
Journal of Dairy Science
Published
2012-07
Volume
95
Issue
7
Pages
3579-3592
ISSN
0022-0302 (ISSN-L)
OpenAlex
W1972070675
Cited by
151
References
50
Citations by year
2026: 9 · 2025: 16 · 2024: 10 · 2023: 13 · 2022: 9 · 2021: 20 · 2020: 13 · 2019: 8 · 2018: 6 · 2017: 7 · 2016: 13 · 2015: 9 · 2014: 6 · 2013: 8 · 2012: 3
FWCI
12.79
Type
journal article
Language
en
Open access
Locations
  • Journal of Dairy Science (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Tertiary Education Commission
  • Ministry of Education- New Zealand
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Proteins in Food Systems
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Ruminant Nutrition and Digestive Physiology
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Animal Diversity and Health Studies
Keywords
in vitro digestion, gastric digestion, intestinal digestion, β-lactoglobulin, pepsin resistance, trypsin hydrolysis, chymotrypsin hydrolysis, lipolysis, micelles, bile salts, phospholipids, peptides, fat globule structure
MeSH terms
Animals, Cattle, Chymotrypsin, Digestion, Glycolipids, Glycoproteins, Lactoglobulins, Lipolysis, Micelles, Milk Proteins, Trypsin, Microscopy, Confocal, Proteolysis, Lipid Droplets, In Vitro Techniques

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