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Effects of oregano essential oil on in vitro ruminal fermentation, methane production, and ruminal microbial community
Open accessAbstract
Different inclusion rates of oregano essential oil (OEO) were investigated for their effects on ruminal in vitro fermentation parameters, total gas, methane production, and bacterial communities. Treatments were (1) control, 0 mg/L of OEO (CON); 13 mg/L (OEO1); 52 mg/L (OEO2); 91 mg/L (OEO3); and 130 mg/L (OEO4), each incubated with 150 mL of buffered rumen fluid and 1,200 mg of substrate for 24 h using the Ankom in vitro gas production system (Ankom Technology Corp., Fairport, NY). Treatment responses were statistically analyzed using polynomial contrasts. Digestibility of DM, NDF, and ADF increased quadratically with increasing OEO inclusion rates. Digestibility of DM and NDF were highest for OEO2, whereas ADF digestibility was highest for OEO3, compared with CON, with the remaining treatments being intermediate and similar. Ammonia nitrogen concentrations decreased from CON at a quadratic rate with increasing OEO inclusion rates, and OEO2 had the lowest concentration compared with the other groups. Total VFA, acetate, propionate, butyrate, valerate, and isovalerate concentrations linearly decreased with increasing OEO inclusion rates. Total gas production levels by CON and OEO4 were greater than those of OEO1, OEO2, and OEO3 in a quadratic response, and methane production linearly decreased from CON, compared with OEO4, at a decreasing rate with OEO inclusion rates. As determined by 16S rRNA sequencing, the α biodiversity of ruminal bacteria was similar among OEO inclusion rates. Increasing OEO inclusion rates linearly increased the relative abundance of Prevotella and Dialister bacteria. Several bacteria demonstrated different polynomial responses, whereas several bacteria were similar among increasing OEO inclusion rates. These results suggested that OEO supplementation can modify ruminal fermentation to alter VFA concentrations and reduce methane emissions by extensively altering the ruminal bacterial community, suggesting an optimal feeding rate for future animal studies of approximately 52 mg/L for mature ruminants.
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“Recently, Zhou et al. (239) suggested that oregano EO (52 mg/L) in mature ruminants can modify ruminal fermentation and mitigate in vitro CH4 production through mediating ruminal bacteria (Prevotella and Dialister).”
From Phytogenic Additives Can Modulate Rumen Microbiome to Mediate Fermentation Kinetics and Methanogenesis Through Exploiting Diet–Microbe Interaction (2020) · cites this paper as [239]
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Cites
R: A Language and Environment for Statistical Computing
R Core Team (2000) · doi:10.32614/r.manuals · Cited by 355,893
Official Methods of Analysis of
Helrick, K. (1980) · Analytical Chemistry · doi:10.1021/ac50052a726 · Cited by 59,351
Van Soest, P. J., Robertson, J. B., & Lewis, B. A. (1991) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(91)78551-2 · Cited by 28,658
Search and clustering orders of magnitude faster than BLAST
Edgar, R. C. (2010) · Bioinformatics · doi:10.1093/bioinformatics/btq461 · Cited by 21,098
FLASH: fast length adjustment of short reads to improve genome assemblies
Magoč, T., & Salzberg, S. L. (2011) · Bioinformatics · doi:10.1093/bioinformatics/btr507 · Cited by 15,366
UCHIME improves sensitivity and speed of chimera detection
Edgar, R. C., Haas, B. J., Clemente, J. C., Quince, C., & Knight, R. (2011) · Bioinformatics · doi:10.1093/bioinformatics/btr381 · Cited by 15,114
Essential oils: Their antibacterial properties and potential applications in foods—A review
Burt, S. (2004) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2004.03.022 · Cited by 10,488
Biological effects of essential oils—A review
Bakkali, F., Averbeck, S., Averbeck, D., & Idaomar, M. (2008) · Food and Chemical Toxicology · doi:10.1016/j.fct.2007.09.106 · Cited by 7,929
Modified Reagents for Determination of Urea and Ammonia
Chaney, A. L., & Marbach, E. P. (1962) · Clinical Chemistry · doi:10.1093/clinchem/8.2.130 · Cited by 3,941
Kh, M. (1988) · Medical Entomology and Zoology · Cited by 2,404
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Research works printed but not linked: 1
- Tekippe, 2010, Effects of herbs and essential oils on in vitro batch culture ruminal fermentation, J. Dairy Sci., 93, 175
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Items 101–129 of 129
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Khusro, A., Aarti, C., Elghandour, M. M. M. Y., Adegbeye, M. J., Mellado, M., Barbabosa‐Pliego, A., Rivas-Cáceres, R. R., & Salem, A. Z. M. (2021) · Springer eBooks · doi:10.1007/978-1-4614-6431-0_131-1 · Cited by 4
Önel, S. E., Kara, K., Gül, M., Öztaş, M. A., & YILMAZ, S. (2021) · Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi · doi:10.47495/okufbed.984443
Εταιρεία, Ε. Κ. (2021) · Journal of the Hellenic Veterinary Medical Society · doi:10.12681/jhvms.26784
Riasi, A., Karimian, M., & Ghasemi, E. (2023) · SSRN Electronic Journal · doi:10.2139/ssrn.4635246
Effect of different concentrations of oregano (Origanum vulgare) extract on rumen digestion in vitro
Ivanishcheva, A. P., Sizova, E. A., Kamirova, А. М., Shoshin, D. E., & Yausheva, Е. (2023) · Siberian Herald of Agricultural Science · doi:10.26898/0370-8799-2023-12-9
Видев, Е., Ангелова, Т., & Krustanov, J. (2024) · Zhivotnov"dni Nauki · doi:10.61308/hdzx9447 · Cited by 1
Xi, W., Li, F., Cai, X., & Haitao, S. (2024) · SSRN Electronic Journal · doi:10.2139/ssrn.4868869
Effect of essential oils and prebiotic on serum biochemical parameters of crossbred lactating cattle
Sarma, G., Palod, J., Sharma, R. K., Kumar, S., Anita, A., Singh, S. K., SHARMA, S., & Gangwar, S. (2024) · Annals of Phytomedicine An International Journal · doi:10.54085/ap.2024.13.1.143
Mahdavi, A., & Darabighane, B. (2024) · Climate · doi:10.3390/cli12100167
Torres, V., Ángulo, L. A. M., & Cárdenas, J. E. G. (2025) · Revista Mvz Córdoba · doi:10.21897/rmvz.3578
Effects of copaiba oleoresin on in vitro ruminal fermentation in bovine
Coelho, G. D. J., Vargas, J. A. C., da Silva, M. A., Santos, V. F. S., da Silva, T. R. C., de Souza, H. G. S., Rosa, J. J. D. S., de Araújo, T. C., de Sousa, S. V., Maciel, R. P., et al. (2025) · Revista Mvz Córdoba · doi:10.21897/rmvz.3532
Wang, J., Yu, X., Zhang, Y., Yang, Y., Sun, Y., & Zhang, Y. (2025) · Animals · doi:10.3390/ani15162332
Muñoz‐Grein, J., López, R., Solanas, E., Fondevila, M., & Belanche, A. (2025) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.70359
Kim, H., Park, T., Kwon, I., & Seo, J. (2021) · Figshare · doi:10.6084/m9.figshare.15102472
Aceite esencial de orégano como alternativa a los antibióticos en rumiantes
López-Puga, J. C., Sobrevilla-Hernández, G., Kawas, J. R., & Méndez‐Zamora, G. (2026) · Ciencia UANL · doi:10.29105/cienciauanl28.135-5
Tang, L., Liu, H., Gao, Q., Sun, Y., Xu, X., Li, W., Lu, D., Kong, L., Liu, S., & Jiang, T. (2026) · Veterinary Sciences · doi:10.3390/vetsci13010108
KHOLIF, A. E., Adegbeye, M. J., Gouda, G. A., Morsy, T. A., & Vargas‐Bello‐Pérez, E. (2026) · Annals of Animal Science · doi:10.2478/aoas-2025-0118
Merkhan, K. Y., & Chaudhry, A. S. (2026) · Anaerobe · doi:10.1016/j.anaerobe.2026.103046
Zain, M., Despal, D., Tanuwiria, U. H., Yunilas, Y., Pazla, R., Putri, E. M., Yanti, G., Ikhlas, Z., Sari, R. R., Sucitra, L. S., et al. (2026) · Veterinary World · doi:10.14202/vetworld.2026.1914-1931
Dibennardo, F., Infascelli, L., Iommelli, P., Tudisco, R., Iasi, A., Musco, N., & Infascelli, F. (2026) · Animals · doi:10.3390/ani16111649
Wang, L. W., Zhao, X. B., Wang, J., Yang, S., Wang, P. P., Li, H. C., Han, S. B., Zhong, R. Z., & Jiang, L. (2026) · animal · doi:10.1016/j.animal.2026.101892
Pachura, N., Banaszkiewicz, S., Lewandowska, K., Burek, A., Szumny, A., Tabiś, A., Bania, J., & Kupczyński, R. (2026) · Molecules · doi:10.3390/molecules31142403 · Cited by 1
Tang, L., Liu, H., Gao, Q., Sun, Y., Xu, X., Li, W., Lu, D., Ren, Y., Yu, H., & Jiang, T. (2026) · PLoS ONE · doi:10.1371/journal.pone.0353206
Jalal, H., Pompei, L., Giammarco, M., Malik, M. I., Akram, S., Akram, M. Z., Domenico, M. D., Ghiaccio, F., Colleluori, R., Pezzi, P., et al. (2026) · Frontiers in Veterinary Science · doi:10.3389/fvets.2026.1900394
Khattab, I. M., Gabr, S. A., El-Hendawy, N. M., Nofel, N. A., Elwakeel, E. A., & Atia, S. E. S. (2026) · Archives of Animal Nutrition · doi:10.1080/1745039x.2026.2710977
Hnokaew, P., Arjin, C., Vinitchaikul, P., Therdtatha, P., Chuammitri, P., Mektrirat, R., Inthasan, J., Satsook, A., & Sringarm, K. (2026) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2026.117102
Metadata
- Authors
- Zhou, R., Wu, J., Lang, X., Liu, L., Casper, D. P., Wang, C., Zhang, L., & Wei, S.
- Affiliations
- Gansu Agricultural University (CN)
- Gansu Academy of Agricultural Sciences (CN)
- Corresponding author
- Wu, Jianping
- Venue
- Journal of Dairy Science
- Publisher
- American Dairy Science Association
- Published
- 2020-03
- Volume
- 103
- Issue
- 3
- Pages
- 2303-2314
- ISSN
- 0022-0302 (ISSN-L)
- OpenAlex
- W2999305564
- Cited by
- 130
- References
- 84
- Citations by year
- 2026: 23 · 2025: 18 · 2024: 24 · 2023: 22 · 2022: 16 · 2021: 19 · 2020: 8
- FWCI
- 20.31
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Dairy Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (CARS-39, 31460592)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Ruminant Nutrition and Digestive Physiology
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Keywords
- oregano essential oil, rumen fermentation, methane mitigation, volatile fatty acids, in vitro gas production, forage digestibility, ammonia nitrogen, Prevotella, Dialister, ruminant feed additives, dairy cattle
- MeSH terms
- Gastrointestinal Microbiome, Ammonia, Animals, Animal Feed, Bacteria, Cattle, Diet, Digestion, Female, Fermentation, Male, Methane, Nitrogen, Oils, Volatile, Plant Oils, RNA, Ribosomal, 16S, Rumen, Sheep, Origanum
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