The Effect of Treating Alfalfa with Lactobacillus buchneri 40788 on Silage Fermentation, Aerobic Stability, and Nutritive Value for Lactating Dairy Cows
Open accessAbstract
Lactobacillus buchneri 40788 and enzymes (beta-glucanase, alpha-amylase, xylanase, and galactomannase) were applied to chopped alfalfa (39% DM) to study their effects on the fermentation and nutritive value of the silage. Alfalfa was treated with nothing, or L. buchneri 40788, for a final application rate of 1 x 10(5), 5 x 10(5), or 1 x 10(6) cfu/g of fresh forage and ensiled in laboratory silos for 2, 4, 8, and 56 d. Treatment with L. buchneri 40788 had few effects on the end products of fermentation through 8 d of ensiling. However, after 56 d of ensiling, treated silages had a higher pH (4.55 vs. 4.38) and higher concentrations of acetic acid (6.40 vs. 4.24%), propionic acid (0.18 vs. 0.06%), and ammonia-N (0.35 vs. 0.29%) when compared to untreated silage. Lactic acid was also numerically lower in treated (3.51%) than untreated (4.12%). Silages treated with the moderate and highest dose of L. buchneri 40788 also resulted in greater recoveries of DM than did untreated silage. Alfalfa (43% DM) was also untreated or treated with a commercial application of L. buchneri 40788 (4 x 10(5) cfu/g, a commercial dose) in farm-scale bag silo. Holstein cows were fed a diet comprised of 32% untreated or treated alfalfa silage, 11% corn silage, 5% chopped alfalfa hay, and 52% of concentrate (DMB) for a 6-wk treatment period. Dry matter intake and milk composition were unaffected by treatment, but cows fed silage treated with L. buchneri 40788 produced 0.8 kg more milk than did cows fed untreated silage. Treated silage had a higher concentration of acetic acid (5.67 vs. 3.35%) but lower lactic acid (3.50 vs. 4.39%) than untreated silage. When exposed to air, the total mixed ration containing treated alfalfa silage remained stable for 100 h, whereas the ration containing untreated silage spoiled after 68 h. Treating alfalfa silage with L. buchneri 40788 increased the concentration of acetic acid, and when the silage was combined into a total mixed ration and fed to lactating cows, it improved the aerobic stability of the ration and increased milk production.
AI snapshot
Written from the paper’s full text.
TL;DR
Treating alfalfa with *Lactobacillus buchneri* 40788 increased acetic acid and altered fermentation after 56 d of ensiling, and improved aerobic stability of the resulting TMR. In a lactation study, cows fed treated alfalfa silage had similar dry matter intake and milk composition but produced 0.8 kg more milk per day than cows fed untreated silage.
Key findings
- After 56 d in laboratory silos, *L. buchneri* 40788-treated alfalfa silage had higher pH (4.55 vs. 4.38), acetic acid (6.40 vs. 4.24%), propionic acid (0.18 vs. 0.06%), and ammonia-N (0.35 vs. 0.29%) than untreated silage; lactic acid was numerically lower in treated silage (3.51 vs. 4.12%).
- Dry matter recovery was greater for silages treated with the moderate (97.6%) and high (93.4%) doses of *L. buchneri* 40788 than for untreated silage (86.8%).
- In the lactation study, TMR containing treated alfalfa silage had improved aerobic stability (68 vs. 100 h) compared with TMR containing untreated silage.
- Dry matter intake and milk composition were unaffected by treatment, but cows fed treated silage produced 0.8 kg more milk per day and tended to produce more FCM/d than cows fed untreated silage.
Methods
- Laboratory silo study: chopped alfalfa (about 39% DM) was untreated or treated with *L. buchneri* 40788 at 1 × 10⁵, 5 × 10⁵, or 1 × 10⁶ cfu/g fresh forage plus enzymes, packed into PVC laboratory silos in triplicate, and opened after 2, 4, 8, and 56 d for microbial, chemical, and DM recovery analyses.
- Lactation study: alfalfa (about 40–43% DM) was stored untreated or treated with *L. buchneri* 40788 at 4 × 10⁵ cfu/g in a farm-scale bag silo for 10 mo; 30 Holstein cows (24 multiparous, 6 primiparous; 81 ± 24 DIM; about 39 kg/d milk) were fed a TMR with 32% untreated or treated alfalfa silage, 11% corn silage, 5% chopped alfalfa hay, and 52% concentrate for a 6-wk treatment period.
- Analyses used GLM in SAS: laboratory silo data as a randomized complete block design with treatment and block (day) as main effects; lactation data as a completely randomized design with pretreatment data as covariate; means compared with Tukey’s test at P < 0.05.
Limitations
- The paper does not state a limitations section.
- Evident from the text: the laboratory silo study used small PVC silos (0.203 × 0.064 m) in triplicate, which may not fully represent farm-scale silage conditions.
- Evident from the text: the lactation study used 30 cows in one 6-wk trial with a single farm-scale bag silo and moderate ambient temperatures (24–30°C); yeasts were not detected in the silages alone, so the aerobic stability benefit was observed only after treated silage was mixed into a TMR.
Model-written summary. Check it against the paper before relying on it.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
0 of 15 cited sources checked · 0 supported · 0 flagged
- Findingown data
Yeasts and molds were not detectable in any of the laboratory silages at any sampling time.
“Yeasts and molds were not detectable in any of the laboratory silages at any sampling time (data not shown).”
Quote in the paperNot a substantial claim - Findingown data
In the lactation study, the water-soluble carbohydrate concentration of alfalfa silage treated with L. buchneri 40788 was lower than that of untreated silage.
“Silage pH was higher, and WSC concentration was lower in silage treated with L. buchneri 40788.”
Quote in the paperNot a substantial claim - Findingown data
In the lactation study, the pH of alfalfa silage treated with L. buchneri 40788 was higher than that of untreated silage.
“Silage pH was higher, and WSC concentration was lower in silage treated with L. buchneri 40788.”
Quote in the paperNot a substantial claim - Findingown data
After 56 d of ensiling in laboratory silos, alfalfa silage treated with L. buchneri 40788 had a higher pH than untreated silage (4.55 vs. 4.38).
“However, after 56 d of ensiling, treated silages had a higher pH (4.55 vs. 4.38)”
- Also stated in: § Laboratory Silo Study · plain
“Silage pH and ammonia-N content was higher in silages treated with L. buchneri 40788.”
Quote in the paperSubstantial claim - Also stated in: § Laboratory Silo Study · plain
- Findingown data
After 4 d of ensiling, alfalfa silages treated with L. buchneri 40788 had lower pH than untreated silage.
“After 4 d of ensiling (Table 5), treated silages had lower pH than did untreated silage”
Quote in the paperSubstantial claim
- Findingown data
When mixed into a total mixed ration, alfalfa silage treated with L. buchneri 40788 improved the aerobic stability of the TMR compared with untreated silage (68 vs. 100 h).
“however, when mixed into a TMR, silages treated with L. buchneri 40788 showed an improvement in aerobic stability of the TMR (68 vs. 100 h).”
Quote in the paperSubstantial claim - Findingown data
After 56 d of ensiling, alfalfa silage treated with L. buchneri 40788 had a higher concentration of acetic acid than untreated silage (6.40 vs. 4.24%).
“and higher concentrations of acetic acid (6.40 vs. 4.24%), propionic acid (0.18 vs. 0.06%), and ammonia-N (0.35 vs. 0.29%) when compared to untreated silage.”
- Also stated in: § Laboratory Silo Study · plain
“The concentration of acetic acid was similar (average of 6.40%) but higher among silages treated with L. buchneri 40788 compared with control silage (4.24%).”
Quote in the paperSubstantial claim - Also stated in: § Laboratory Silo Study · plain
- Findingown data
Cows fed alfalfa silage treated with L. buchneri 40788 produced 0.8 kg more milk per day than cows fed untreated silage.
“but cows fed silage treated with L. buchneri 40788 produced 0.8 kg more milk than did cows fed untreated silage.”
Quote in the paperSubstantial claim - Findingown data
In the laboratory silo study, treatment of alfalfa with L. buchneri 40788 had few effects on the end products of fermentation through 8 d of ensiling.
“Treatment with L. buchneri 40788 had few effects on the end products of fermentation through 8 d of ensiling.”
Quote in the paperNot a substantial claim - Findingown data
After 56 d of ensiling, dry matter recovery was greater in alfalfa silage treated with the moderate (97.6%) and high (93.4%) levels of L. buchneri 40788 than in untreated silage (86.8%).
“Silages treated with the moderate and highest dose of L. buchneri 40788 also resulted in greater recoveries of DM than did untreated silage.”
- Also stated in: § Laboratory Silo Study · plain
“After 56 d of ensiling, DM recovery was greater in silages treated with the moderate (97.6%) and high (93.4%)levels of L. buchneri 40788 compared with control silage (86.8%;Table 7).”
Quote in the paperSubstantial claim - Also stated in: § Laboratory Silo Study · plain
- Findingown data
Cows fed silage treated with L. buchneri 40788 tended to produce more fat-corrected milk per day than cows fed untreated silage.
“but cows fed silage treated with L. buchneri 40788 produced more milk per day and tended to produce more FCM/d than did cows fed untreated silage.”
Quote in the paperSubstantial claim - Findingown data
After 8 d of ensiling, lactic acid concentration was lowest in silage treated with the low level of L. buchneri 40788.
“The concentration of lactic acid was lowest in silage treated with the low level of L. buchneri 40788 after 8 d of fermentation”
Quote in the paperNot a substantial claim - Findingreasoning
Alfalfa silages stored in laboratory and farm silos did not heat when exposed to air.
“Silages did not heat when exposed to air (data not shown);”
- Also stated in: § DISCUSSION · plain
“Yeasts were not detected in any of the alfalfa silages stored in laboratory and farm silos and, thus, alfalfa silages alone did not heat (data not shown).”
Quote in the paperNot a substantial claim - Also stated in: § DISCUSSION · plain
- Findingown data
After 56 d of ensiling, ethanol concentration was lower in untreated silage (0.45%) than in silage treated with the highest level of L. buchneri 40788 (0.62%).
“The concentration of ethanol was lower in untreated silage (0.45%) than in silage treated with the highest level of L. buchneri 40788 (0.62%).”
Quote in the paperNot a substantial claim - Findingown data
After 56 d of ensiling, lactic acid concentration was numerically lower in silage treated with L. buchneri 40788 (3.51%) than in untreated silage (4.12%).
“Lactic acid was also numerically lower in treated (3.51%) than untreated (4.12%).”
Quote in the paperNot a substantial claim - Findingown data
After 56 d of ensiling, alfalfa silage treated with L. buchneri 40788 had a higher concentration of propionic acid than untreated silage (0.18 vs. 0.06%).
“and higher concentrations of acetic acid (6.40 vs. 4.24%), propionic acid (0.18 vs. 0.06%), and ammonia-N (0.35 vs. 0.29%) when compared to untreated silage.”
- Also stated in: § Laboratory Silo Study · plain
“Silage treated with the low and moderate level of L. buchneri 40788 also had higher concentrations (average 0.21%) of propionic acid than did control silage (0.06%).”
Quote in the paperSubstantial claim - Also stated in: § Laboratory Silo Study · plain
- Findingown data
After 56 d of ensiling, alfalfa silage treated with L. buchneri 40788 had a higher concentration of ammonia-N than untreated silage (0.35 vs. 0.29%).
“and higher concentrations of acetic acid (6.40 vs. 4.24%), propionic acid (0.18 vs. 0.06%), and ammonia-N (0.35 vs. 0.29%) when compared to untreated silage.”
Quote in the paperNot a substantial claim - Findingown data
After 2 d of ensiling, ammonia-N was greater in silage treated with the highest level of L. buchneri 40788 than in the other treatments.
“The composition of silages was similar among treatments, with the exception that ammonia-N was greater in silage treated with the highest level of L. buchneri 40788.”
Quote in the paperNot a substantial claim - Interpretationown data
High concentrations of acetic acid in silage treated with L. buchneri 40788 do not limit intake of lactating dairy cows.
“suggesting that high concentrations of acetic acid in silage treated with L. buchneri 40788 do not limit intake.”
Quote in the paperSubstantial claim - Study design and conductown data
The chemical and microbial composition of fresh alfalfa before ensiling was similar among treatments, except for small differences in dry matter content.
“The chemical and microbial content of fresh alfalfa before ensiling was similar among treatments with the exception of small differences in the DM content (Table 3).”
Quote in the paperNot a substantial claim - Interpretationreasoning
L. buchneri 40788 improves the aerobic stability of silages.
“This study further supports the concept that L. buchneri 40788 improves the aerobic stability of silages”
Quote in the paperSubstantial claim - Premisecitation
Anaerobic conversion of lactic acid to acetic acid, 1,2-propanediol, and ethanol by L. buchneri 40788 occurs only at low pH.
“The probable reason for this finding is that anaerobic conversion of lactic acid to acetic acid, 1,2 propanediol, and ethanol by L. buchneri 40788 occurs only at a low pH (Oude Elferink et al., 2001).”
Quote in the paperSubstantial claim0 of 1 sources checked- cites Oude Elferink et al., 2001 source
Not checked in the free run.
- Premisecitation
Excess air trapped in the forage mass compromises the ensiling process by promoting the growth of aerobic microorganisms.
“Excess air trapped in the forage mass severely compromises the subsequent ensiling process by promoting the growth of aerobic microorganisms (Woolford, 1990).”
Quote in the paperSubstantial claim0 of 1 sources checked- cites Woolford, 1990 source
Not checked in the free run.
- Premisecitation
Feeding spoiled silages to cows has been observed to depress intake and performance.
“When spoiled silages are fed to cows, depressions in intake and performance have been observed (Hoffman and Ocker, 1997;Whitlock et al., 2000).”
Quote in the paperSubstantial claim0 of 2 sources checked- cites Hoffman and Ocker, 1997
Not checked in the free run.
- cites Whitlock et al., 2000
Not checked in the free run.
- Interpretationreasoning
When silage treated with L. buchneri 40788 was mixed into a total mixed ration for dairy cows, the ration remained fresh for a longer period than a ration containing untreated silage.
“when treated silage was mixed into a TMR for dairy cows, the ration remained fresher for a longer period of time compared with a ration with untreated silage.”
Quote in the paperSubstantial claim - Interpretationown data
Adding L. buchneri 40788 to alfalfa at ensiling increased the acetic acid concentration of the resulting silage in both laboratory and farm-scale silos.
“The results of our study showed that adding of L. buchneri 40788 to alfalfa at the time of ensiling increased the concentration of acetic acid in alfalfa silage stored in both laboratory and farm silos.”
Quote in the paperSubstantial claim - Premisecitation
Enzymes were ineffective in altering fermentation end products in a previous study by the authors.
“However, in a previous study, we found that enzymes were ineffective in altering ferementation end products (Taylor et al., 2002).”
Quote in the paperNot a substantial claim0 of 1 sources checked- cites Taylor et al., 2002 source
Not checked in the free run.
- May combine two claims. 8
- Classified as probably not a substantial claim. 8
May combine two claims.
Findingown dataIn the alfalfa silage fed during the lactation study, lactic acid concentration was lower and acetic acid concentration was higher in silage treated with L. buchneri 40788 than in untreated silage.
“The concentrations of lactic acid and acetic acid were lower and higher, respectively, in silage treated with L. buchneri 40788”
Quote in the paperSubstantial claimProbably not a substantial claim: may not pass S3 (load-bearing).
Findingown dataUntreated fresh alfalfa contained more lactic acid bacteria (6.85 log10 cfu/g) than the levels applied with the inoculants.
“Untreated alfalfa contained more lactic acid bacteria (6.85 log 10 cfu/g) than levels applied with the inoculants.”
Quote in the paperNot a substantial claimMay combine two claims.
Findingown dataIn the lactation study, dry matter intake and milk composition of cows were unaffected by feeding alfalfa silage treated with L. buchneri 40788.
“Dry matter intake and milk composition were unaffected by treatment, but cows fed silage treated with L. buchneri 40788 produced 0.8 kg more milk than did cows fed untreated”
- Also stated in: § Lactation Study · plain
“Dry matter intake and milk composition were similar between treatments (Table 9), but cows fed silage treated with L. buchneri 40788 produced more milk per day and tended to produce more FCM/d than did cows fed untreated silage.”
Quote in the paperNot a substantial claim- Also stated in: § Lactation Study · plain
Probably not a substantial claim: may not pass S3 (load-bearing). May combine two claims.
PremisecitationIn most silages, the concentration of lactic acid is greater than that of acetic acid, with typical ratios of approximately 3:1.
“In most silages, the concentration of lactic acid is usually greater than the concentration of acetic acid with typical ratios of approximately 3:1 (Ward, 2000).”
Quote in the paperNot a substantial claim0 of 1 sources checked- cites Ward, 2000
Not checked in the free run.
May combine two claims.
PremisecitationTreating silage with L. buchneri 40788 has decreased the ratio of lactic to acetic acid, and in some instances the resulting silage has had more acetic than lactic acid.
“In contrast, treating silage with L. buchneri 40788 has resulted in a decrease in the ratio of lactic:acetic acid, and in some instances the resulting silage has had more acetic than lactic acid (Driehuis et al., 2001).”
Quote in the paperNot a substantial claim0 of 1 sources checked- cites Driehuis et al., 2001 source
Not checked in the free run.
Probably not a substantial claim: may not pass S3 (load-bearing).
Study design and conductcitationDiets in the lactation study were balanced to meet National Research Council (2001) nutrient requirements.
“Diets were balanced to meet National Research Council (2001) requirements (Table 2).”
Quote in the paperNot a substantial claim0 of 1 sources checked- cites National Research Council (2001)
Not checked in the free run.
Classified as probably not a substantial claim.
Study design and conductcitationAerobic stability was measured as the number of hours the forage mass remained at baseline temperature before rising 2°C.
“Aerobic stability was defined as the number of h the forage mass remained at baseline temperature before rising 2°C (Moran et al., 1996).”
Quote in the paperSubstantial claim0 of 1 sources checked- cites Moran et al., 1996
Not checked in the free run.
May combine two claims.
Interpretationown dataFeeding lactating cows alfalfa silage treated with L. buchneri 40788, which contained a high concentration of acetic acid, had no effect on dry matter intake but increased milk production.
“The results from the current study showed that feeding alfalfa silage treated with L. buchneri 40788, which contained a high concentration of acetic acid, had no effect on the DMI of lactating cows but increased milk production.”
Quote in the paperSubstantial claimMay combine two claims.
Interpretationown dataIn the laboratory silo study, all inoculation levels of L. buchneri 40788 increased acetic acid concentration, and the effect was not dependent on dose.
“In the laboratory silo study, all levels of inoculation (from 1 × 10 5 to 1 × 10 6 cfu/g) increased the concentration of acetic acid, but the effect was not dependent on dose.”
Quote in the paperNot a substantial claimClassified as probably not a substantial claim.
PremisecitationIn some past studies, silages with high levels of acetic acid have been associated with depressed animal intakes.
“in some past studies, silages with high levels of acetic acid have been associated with depressed animal intakes (Wilkins et al., 1971;Rook and Gill, 1990).”
Quote in the paperSubstantial claim0 of 2 sources checked- cites Wilkins et al., 1971
Not checked in the free run.
- cites Rook and Gill, 1990
Not checked in the free run.
Probably not a substantial claim: may not pass S3 (load-bearing).
Study design and conductcitationThe care of the animals in the lactation study followed accepted protocols.
“the care of animals was via accepted protocols (Anon., 1999).”
Quote in the paperNot a substantial claim0 of 1 sources checked- cites Anon., 1999
Not checked in the free run.
May combine two claims.
PremisecitationL. buchneri 40788 added to forage at harvest has increased acetic acid concentration, lowered yeast numbers, and improved aerobic stability in a variety of silages.
“Lactobacillus buchneri 40788 is a biological alternative that, when added to forage at harvest, has resulted in an increase in the concentration of acetic acid, a lower number of yeasts, and improved aerobic stability in a variety of silages (Muck, 1996;Weinberg et al., 1999;Kung and Ranjit, 2001).”
Quote in the paperSubstantial claim0 of 3 sources checkedClassified as probably not a substantial claim.
Study design and conductown dataCows in the lactation study were randomly allocated into two treatments based on pretreatment milk production, parity, and days in milk.
“Cows were randomly allocated into two treatments based on pretreatment milk production, parity, and DIM and fed a similar diet for a 6wk treatment period with the exception that all the alfalfa silage was either untreated or treated.”
Quote in the paperSubstantial claimProbably not a substantial claim: may not pass S3 (load-bearing).
Study design and conductnone givenData from the pretreatment period were used for covariate adjustment in the lactation study.
“Data from the pretreatment period were used for covariate adjustment.”
Quote in the paperNot a substantial claimMay combine two claims.
Interpretationown dataCows fed a total mixed ration containing alfalfa inoculated with L. buchneri 40788 consumed similar amounts of dry matter as cows fed untreated silage but produced more milk.
“Cows fed a TMR containing alfalfa inoculated with L. buchneri 40788 ate similar amounts of DM as those cows fed untreated silage, but they produced more milk.”
Quote in the paperSubstantial claim
The paper relies on this without support.
Before this study there were no published reports on whether L. buchneri 40788 can improve the aerobic stability of alfalfa silage.
“To date, there are no reports on the ability of L. buchneri 40788 to improve the aerobic stability of alfalfa silage.”
The paper relies on this without support.
Alfalfa silage can form a major portion of the forage in dairy cow diets and can spoil when exposed to air.
“Although research on improving the aerobic stability of forage crops has primarily been focused on corn silage and high-moisture corn, alfalfa silage can be a major portion of the forage in diets for dairy cows, and it can also spoil when exposed to air.”
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
ADFom and animal digestibility are negatively correlated; the lower the value of ADFom, the higher the digestibility of the feed and the higher the feeding value.
“ADFom and animal digestibility are negatively correlated. The lower the value of ADFom, the higher the digestibility of the feed and the higher the feeding value [28].”
From Cellulase Interacts with Lactic Acid Bacteria to Affect Fermentation Quality, Microbial Community, and Ruminal Degradability in Mixed Silage of Soybean Residue and Corn Stover (2021) · cites this paper as [28]
The Lactation Study section says ADF was measured, but the passages found do not relate ADF or ADFom to digestibility or feeding value. The Discussion and Conclusions report intake and milk-production outcomes instead. Searches for ADFom, ADF, and digestibility did not find the claimed negative correlation. (LLM, conf=0.72)
Experimental — accuracy not yet measuredLb. buchneri 40788 improves aerobic stability by reducing yeast counts.
“several authors demonstrated that Lb. buchneri 40788 improves aerobic stability by reducing yeast counts [58].”
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
Each contradiction between two passages listed here was confirmed by an independent check.
Farm-scale inoculant both contained and did not contain enzymes
MajorThe lactation-study methods state that the farm-scale inoculant contained four enzymes, but the discussion says the farm-scale treatment did not include enzymes. This changes the stated basis for explaining differences between the laboratory and farm-scale results.
“The inoculant also contained the following enzymes: β-glucanase (42,000 IU/tonne of fresh forage), α-amylase (21,000 IU/tonne of forage), xylanase (22,800 IU/tonne of forage), and galactomannase (3840 IU/ tonne of forage).”
“Minor differences in response to treatment between treated alfalfa in laboratory and farm-scale silos may have been due to the fact that farm-scale treatment did not include enzymes.”
Dry matter recovery method calculates dry matter loss
MinorSubtracting final DM weight from initial DM weight and dividing by initial weight gives the fraction lost, not the fraction recovered. The reported values are labeled dry matter recovery, so the stated calculation and reported measure are inconsistent.
“Dry matter recovery was calculated by subtracting the final DM weight from the initial DM weight in each silo and then by dividing that number by the initial DM weight in each silo.”
“| DMR 1 , % | 86.8 b | 92.7 ab | 97.6 a | 93.4 a | 1.3 |”
Discussion calls the high-dose recovery increase merely numerical
MinorThe discussion describes the high-dose treatment as only numerically greater than untreated silage. Table 7 assigns the high-dose value and control different significance letters, indicating a statistically significant difference under the table's stated convention.
“In the current study, DM recovery from laboratory silos was greater in silages treated with the moderate level (97.6%) of L. buchneri 40788 and numerically greater in other L. buchneri 40788 treatments (average of 93.1%) when compared to untreated silage (86.8%).”
“| DMR 1 , % | 86.8 b | 92.7 ab | 97.6 a | 93.4 a | 1.3 |”
The citation lists haven’t been fetched yet
References 24 · Cited by 209
Research works printed but not linked: 2
- Hoffman, 1997, Quantification of milk yield losses associated with feeding aerobically unstable high-moisture corn, J. Dairy Sci., 80, 234
- Whitlock, 2000, Effect of level of surface-spoiled silage on the nutritive value of corn silage diets, J. Dairy Sci., 83, 110
- Authors
- Kung, L., Taylor, C. C., Lynch, M. P., & Neylon, J. M.
- Affiliations
- University of Delaware (US)
- Corresponding author
- Kung, L.
- ORCID
- Kung, L.
- Venue
- Journal of Dairy Science
- Publisher
- American Dairy Science Association
- Published
- 2003-01
- Volume
- 86
- Issue
- 1
- Pages
- 336-343
- ISSN
- 0022-0302 (ISSN-L)
- OpenAlex
- W2048568786
- Semantic Scholar
- 9c5e748a861598f320298e9938275af198e10e69
- Cited by
- 209
- References
- 24
- Citations by year
- 2026: 3 · 2025: 8 · 2024: 10 · 2023: 13 · 2022: 15 · 2021: 20 · 2020: 12 · 2019: 4 · 2018: 11 · 2017: 11 · 2016: 8 · 2015: 8 · 2014: 15 · 2013: 9 · 2012: 9
- FWCI
- 11.53
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Dairy Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Delaware
- Topics
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Mycotoxins in Agriculture and Food
- Keywords
- alfalfa silage, silage fermentation, aerobic stability, silage additives, lactating dairy cows, milk yield, milk composition, volatile fatty acids, silage pH, dry matter loss, lactate, lactic acid, acetic acid, propionic acid, ammonia-N, dry matter intake
- MeSH terms
- Aerobiosis, Medicago sativa, Animals, Cattle, Eating, Female, Fermentation, Food Preservation, Hydrogen-Ion Concentration, Lactation, Lactobacillus, Milk, Nutritive Value, Propionates, Silage, Time Factors, Colony Count, Microbial, Acetic Acid, Lactic Acid
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