Cited by 2Animal Nutrition and PhysiologyGut microbiota and healthMicrobial Metabolites in Food Biotechnology

Chitooligosaccharides improves intestinal mucosal immunity and intestinal microbiota in blue foxes

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Wei, Jiali, Su, Jing, Wang, Guiwu, Li, Wei, Wen, Zhengshun, Liu, Huitao·2024·Frontiers in Immunology

Abstract

Objective: Gut health is critical to the health of the host. This study was conducted to investigate the effects of Chitooligosaccharides (COS) on intestinal morphology, intestinal barrier, intestinal immunity and cecum microbiota of blue foxes. Methods: Seventy-two 125-day-old blue foxes were randomly divided into basal diet (BD) group, 200 ppm COS1 (1.5 kDa) group and 200 ppm COS2 (3 kDa) group for 8 weeks. Results: We elucidated that dietary COS1 supplementation promoted the development of intestinal villus morphology in blue foxes. Importantly, COS1 increased the number of goblet cells in duodenum, jejunum and ileum by 27.71%, 23.67%, 14.97% and S-IgA secretion in duodenum, jejunum and ileum by 71.59% and 38.56%, and up-regulate the expression of Occludin and ZO-1 by 50.18% and 148.62%, respectively. Moreover, COS1 promoted the pro-inflammatory and anti-inflammatory balance of small intestinal mucosa, and increased the diversity of cecum microbiota of blue foxes, especially Lactobacillus_agilis and Lactobacillus_murinus, and up-regulated the signaling pathways related to polysaccharide decomposition and utilization. Conclusion: Here, we present dietary COS1 (1.5 kDa) can promote intestinal villus development, enhance intestinal barrier function, regulate intestinal immune balance and cecum microbiota homeostasis.

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Crossref · 10.3389/fimmu.2024.1506991

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Authors
Wei, J., Su, J., Wang, G., Li, W., Wen, Z., & Liu, H.
Affiliations
  • Chinese Academy of Agricultural Sciences (CN)
  • Shijiazhuang Academy of Agriculture and Forestry Sciences (CN)
Corresponding author
  • Wen, Zhengshun
  • Liu, Huitao
Venue
Frontiers in Immunology
Published
2024-11-19
Volume
15
Pages
1506991-1506991
ISSN
1664-3224 (ISSN-L)
OpenAlex
W4404509532
Cited by
2
References
74
Citations by year
2025: 2
FWCI
0.55
Type
journal article
Language
en
Open access
Locations
  • Frontiers in Immunology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
  • Chinese Academy of Agricultural Sciences (CAAS-ASTIP-2021, CAAS-ASTIP-2021-ISAPS)
  • Bureau of Science and Technology of Zhoushan
  • Agricultural Science and Technology Innovation Program (CAAS-ASTIP-2021- ISAPS)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Nursing › Nutrition and Dietetics › Microbial Metabolites in Food Biotechnology
Keywords
chitooligosaccharides, blue fox, intestinal mucosal immunity, intestinal barrier function, intestinal villus morphology, goblet cells, secretory IgA, occludin, ZO-1, cecal microbiota, Lactobacillus, dietary supplementation, gut health
MeSH terms
Gastrointestinal Microbiome, Animals, Chitin, Foxes, Intestinal Mucosa, Oligosaccharides, Immunity, Mucosal, Chitosan

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