Chitooligosaccharides improves intestinal mucosal immunity and intestinal microbiota in blue foxes
Open accessAbstract
Objective: Gut health is critical to the health of the host. This study was conducted to investigate the effects of Chitooligosaccharides (COS) on intestinal morphology, intestinal barrier, intestinal immunity and cecum microbiota of blue foxes. Methods: Seventy-two 125-day-old blue foxes were randomly divided into basal diet (BD) group, 200 ppm COS1 (1.5 kDa) group and 200 ppm COS2 (3 kDa) group for 8 weeks. Results: We elucidated that dietary COS1 supplementation promoted the development of intestinal villus morphology in blue foxes. Importantly, COS1 increased the number of goblet cells in duodenum, jejunum and ileum by 27.71%, 23.67%, 14.97% and S-IgA secretion in duodenum, jejunum and ileum by 71.59% and 38.56%, and up-regulate the expression of Occludin and ZO-1 by 50.18% and 148.62%, respectively. Moreover, COS1 promoted the pro-inflammatory and anti-inflammatory balance of small intestinal mucosa, and increased the diversity of cecum microbiota of blue foxes, especially Lactobacillus_agilis and Lactobacillus_murinus, and up-regulated the signaling pathways related to polysaccharide decomposition and utilization. Conclusion: Here, we present dietary COS1 (1.5 kDa) can promote intestinal villus development, enhance intestinal barrier function, regulate intestinal immune balance and cecum microbiota homeostasis.
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- Authors
- Wei, J., Su, J., Wang, G., Li, W., Wen, Z., & Liu, H.
- Affiliations
- Chinese Academy of Agricultural Sciences (CN)
- Shijiazhuang Academy of Agriculture and Forestry Sciences (CN)
- Corresponding author
- Wen, Zhengshun
- Liu, Huitao
- Venue
- Frontiers in Immunology
- Publisher
- Frontiers Media SA
- Published
- 2024-11-19
- Volume
- 15
- Pages
- 1506991-1506991
- ISSN
- 1664-3224 (ISSN-L)
- PMC
- PMC11611864
- OpenAlex
- W4404509532
- Cited by
- 2
- References
- 74
- Citations by year
- 2025: 2
- FWCI
- 0.55
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Frontiers in Immunology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Chinese Academy of Agricultural Sciences (CAAS-ASTIP-2021, CAAS-ASTIP-2021-ISAPS)
- Bureau of Science and Technology of Zhoushan
- Agricultural Science and Technology Innovation Program (CAAS-ASTIP-2021- ISAPS)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Nursing › Nutrition and Dietetics › Microbial Metabolites in Food Biotechnology
- Keywords
- chitooligosaccharides, blue fox, intestinal mucosal immunity, intestinal barrier function, intestinal villus morphology, goblet cells, secretory IgA, occludin, ZO-1, cecal microbiota, Lactobacillus, dietary supplementation, gut health
- MeSH terms
- Gastrointestinal Microbiome, Animals, Chitin, Foxes, Intestinal Mucosa, Oligosaccharides, Immunity, Mucosal, Chitosan
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