SNF3 as high affinity glucose sensor and its function in supporting the viability of Candida glabrata under glucose-limited environment
Open accessAbstract
Candida glabrata is an emerging human fungal pathogen that has efficacious nutrient sensing and responsiveness ability. It can be seen through its ability to thrive in diverse range of nutrient limited-human anatomical sites. Therefore, nutrient sensing particularly glucose sensing is thought to be crucial in contributing to the development and fitness of the pathogen. This study aimed to elucidate the role of SNF3 (Sucrose Non Fermenting 3) as a glucose sensor and its possible role in contributing to the fitness and survivability of C. glabrata in glucose-limited environment. The SNF3 knockout strain was constructed and subjected to different glucose concentrations to evaluate its growth, biofilm formation, amphotericin B susceptibility, ex vivo survivability and effects on the transcriptional profiling of the sugar receptor repressor (SRR) pathway-related genes. The CgSNF3Δ strain showed a retarded growth in low glucose environments (0.01 and 0.1%) in both fermentation and respiration-preferred conditions but grew well in high glucose concentration environments (1 and 2%). It was also found to be more susceptible to amphotericin B in low glucose environment (0.1%) and macrophage engulfment but showed no difference in the biofilm formation capability. The deletion of SNF3 also resulted in the down-regulation of about half of hexose transporters genes (four out of nine). Overall, the deletion of SNF3 causes significant reduction in the ability of C. glabrata to sense limited surrounding glucose and consequently disrupts its competency to transport and perform the uptake of this critical nutrient. This study highlighted the role of SNF3 as a high affinity glucose sensor and its role in aiding the survivability of C. glabrata particularly in glucose limited environment.
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C. glabrata is able to grow in very low concentration of glucose, and its glucose sensing pathway resembles that of S. cerevisiae.
“C. glabrata is also able to grow in very low concentration of glucose, and its glucose sensing pathway resembles to that of S. cerevisiae [113].”
From Candida glabrata: A Lot More Than Meets the Eye (2019) · cites this paper as [113]
CgSnf3 acts as the high-affinity glucose sensor which is essential for growth under glucose-limited conditions and in macrophages.
“CgSnf3 acts as the high-affinity glucose sensor which is essential for growth under glucose-limited conditions and in macrophages [113].”
From Candida glabrata: A Lot More Than Meets the Eye (2019) · cites this paper as [113]
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- Authors
- Ng, T. S., Chew, S. Y., Rangasamy, P., Mohd Desa, M. N., Sandai, D., Chong, P. P., & Than, L. T. L.
- Affiliations
- Universiti Putra Malaysia (MY)
- Universiti Sains Malaysia (MY)
- Corresponding author
- Than, Leslie Thian Lung
- Venue
- Frontiers in Microbiology
- Publisher
- Frontiers Media SA
- Published
- 2015-12-01
- Volume
- 6
- Pages
- 1334-1334
- ISSN
- 1664-302X (ISSN-L)
- PMC
- PMC4664639
- OpenAlex
- W2193097080
- Cited by
- 15
- References
- 35
- Citations by year
- 2024: 1 · 2023: 1 · 2022: 1 · 2021: 3 · 2020: 4 · 2019: 4 · 2016: 1
- FWCI
- 0.14
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Frontiers in Microbiology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Universiti Putra Malaysia Institutional Repository (Universiti Putra Malaysia) (acceptedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Greater South Information System
- Funders
- Universiti Putra Malaysia (04-02-12-1799RU)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
- Keywords
- Snf3, Candida glabrata, glucose detection, nutrient sensing, hexose transporters, amphotericin B susceptibility, macrophage engulfment, biofilm formation, fungal pathogen survival, gene expression profiling
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