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Cited by 163Fungal and yeast genetics researchMicrobial Metabolic Engineering and BioproductionFermentation and Sensory Analysis

Transient-state analysis of metabolic fluxes in crabtree-positive and crabtree-negative yeasts

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Van Urk, Hendrik, Voll, W. S. Leopold, Scheffers, W. Alexander, Van Dijken, Johannes P.·1990·Applied and Environmental Microbiology
DOI 10.1128/aem.56.1.281-287.1990PMC PMC183316
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Abstract

In bakers' yeast, an immediate alcoholic fermentation begins when a glucose pulse is added to glucose-limited, aerobically grown cells. The mechanism of this short-term Crabtree effect was investigated via a comparative enzymic analysis of eight yeast species. It was established that the fermentation rate of the organisms upon transition from glucose limitation to glucose excess is positively correlated with the level of pyruvate decarboxylase (EC 4.1.1.1). In the Crabtree-negative yeasts, the pyruvate decarboxylase activity was low and did not increase when excess glucose was added. In contrast, in the Crabtree-positive yeasts, the activity of this enzyme was on the average sixfold higher and increased after exposure to glucose excess. In Crabtree-negative species, relatively high activities of acetaldehyde dehydrogenases (EC 1.2.1.4 and EC 1.2.1.5) and acetyl coenzyme A synthetase (EC 6.2.1.1), in addition to low pyruvate decarboxylase activities, were present. Thus, in these yeasts, acetaldehyde can be effectively oxidized via a bypass that circumvents the reduction of acetaldehyde to ethanol. Growth rates of most Crabtree-positive yeasts did not increase upon transition from glucose limitation to glucose excess. In contrast, the Crabtree-negative yeasts exhibited enhanced rates of biomass production which in most cases could be ascribed to the intracellular accumulation of reserve carbohydrates. Generally, the glucose consumption rate after a glucose pulse was higher in the Crabtree-positive yeasts than in the Crabtree-negative yeasts. However, the respiratory capacities of steady-state cultures of Crabtree-positive yeasts were not significantly different from those of Crabtree-negative yeasts. Thus, a limited respiratory capacity is not the primary cause of the Crabtree effect in yeasts. Instead, the difference between Crabtree-positive and Crabtree-negative yeasts is attributed to differences in the kinetics of glucose uptake, synthesis of reserve carbohydrates, and pyruvate metabolism.

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Crossref · 10.1128/aem.56.1.281-287.1990

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  • C. glabrata is a facultatively anaerobic, crabtree-positive yeast which prefers fermentation over respiration in the presence of oxygen.

    “C. glabrata is a facultatively anaerobic, crabtree-positive yeast which prefers fermentation over respiration in the presence of oxygen [110,111].”

    From Candida glabrata: A Lot More Than Meets the Eye (2019) · cites this paper as [110]

  • C. glabrata is a Crabtree-positive fungus, and its respiration could be negatively affected by certain levels of glucose.

    “Moreover, C. glabrata is a Crabtree-positive fungus [220], thus its respiration could be negatively affected by certain levels of glucose [221].”

    From Candida glabrata Biofilms: How Far Have We Come? (2017) · cites this paper as [221]

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Authors
Van Urk, H., Voll, W. S. L., Scheffers, W. A., & Van Dijken, J. P.
Affiliations
  • Department of Microbiology and Enzymology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands
  • Delft University of Technology (NL)
Venue
Applied and Environmental Microbiology
Publisher
American Society for Microbiology
Published
1990-01
Volume
56
Issue
1
Pages
281-287
ISSN
  • 0099-2240 (ISSN-L)
  • 1098-5336
DOI
10.1128/aem.56.1.281-287.1990
PMC
PMC183316
OpenAlex
W2117697356
Cited by
163
References
25
Citations by year
2025: 2 · 2024: 1 · 2023: 2 · 2022: 4 · 2021: 4 · 2020: 5 · 2019: 6 · 2018: 5 · 2017: 4 · 2016: 9 · 2015: 8 · 2014: 12 · 2013: 6 · 2012: 8
FWCI
1.43
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Applied and Environmental Microbiology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Research Repository (Delft University of Technology) (submittedVersion)
  • Data Archiving and Networked Services (DANS) (submittedVersion, other-oa)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Fermentation and Sensory Analysis
Keywords
Crabtree effect, yeast metabolism, pyruvate decarboxylase, acetaldehyde dehydrogenase, acetyl coenzyme A synthetase, glucose pulse, glucose limitation, alcoholic fermentation, carbohydrate reserves, glucose uptake kinetics, respiratory capacity, pyruvate metabolism

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