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Cited by 74Fermentation and Sensory AnalysisYeasts and Rust Fungi StudiesHorticultural and Viticultural Research

Biocontrol Ability and Action Mechanism of Starmerella bacillaris (Synonym Candida zemplinina) Isolated from Wine Musts against Gray Mold Disease Agent Botrytis cinerea on Grape and Their Effects on Alcoholic Fermentation

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Lemos, Wilson J., Bovo, Barbara, Nadai, Chiara, Crosato, Giulia, Carlot, Milena, Favaron, Francesco, Giacomini, Alessio, Corich, Viviana·2016·Frontiers in Microbiology
DOI 10.3389/fmicb.2016.01249PMC PMC4983571
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Abstract

Gray mold is one of the most important diseases of grapevine in temperate climates. This plant pathogen affects plant growth and reduces wine quality. The use of yeasts as biocontrol agents to apply in the vineyard have been investigated in recent years as an alternative to agrochemicals. In this work, fermenting musts obtained from overripe grape berries, therefore more susceptible to infection by fungal pathogens such as Botrytis cinerea, were considered for the selection of yeasts carrying antifungal activity. Thirty-six isolates were identified as Starmerella bacillaris, a species recently proven to be of enological interest. Among them 14 different strains were studied and antifungal activity against B. cinerea was demonstrated, for the first time, to be present in S. bacillaris species. The production of volatile organic compounds (VOCs), tested in vitro, was found to be the main responsible of S. bacillaris antifungal effects. All the strains were able to reduce B. cinerea decay on wounded grape berries artificially inoculated with gray mold. The colonization level of wound was very high reaching, after 5 days, a concentration of 10(6) cells per ml of grape juice obtained after berry crushing. At this cell concentration S. bacillaris strains were used to ferment synthetic and natural musts. The sequential yeast inoculation, performed by adding S. cerevisiae 48 h after S. bacillaris, was needed to complete sugar consumption and determined a significant increase in glicerol content and a reduction of ethanol and acetic acid concentrations. The high wound colonization ability, found in this work, together with the propensity to colonize grape berry and the interesting enological traits possessed by the selected S. bacillaris strains allow the use of this yeast as biocontrol agent on vine and grape berries with possible positive effects on must fermentation, although the presence of S. cerevisiae is needed to complete the fermentation process. This work introduces new possibilities in wine yeast selection programs in order to identify innovative wine yeasts that are simultaneously antifungal agents in vineyards and alternative wine starters for grape must fermentation and open new perspective to a more integrated strategy for increasing wine quality.

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  • Recent experimental evidence has revealed the key role of the yeast volatilome in yeast-pathogen interactions, including postharvest pathogens, and mycotoxin-producing fungi.

    “Recent experimental evidence has revealed the key role of the yeast volatilome in yeast-pathogen interactions, including postharvest pathogens, and mycotoxin-producing fungi (Supplementary Table 1) (Bruce et al. 2003; Lemos Jr 2016; Parafati et al. 2015).”

    From Biocontrol yeasts: mechanisms and applications (2019) · cites this paper as Lemos Jr 2016

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Authors
Lemos, W. J., Bovo, B., Nadai, C., Crosato, G., Carlot, M., Favaron, F., Giacomini, A., & Corich, V.
Affiliations
University of Padua (IT)
Corresponding author
Giacomini, Alessio
ORCID
Venue
Frontiers in Microbiology
Publisher
Frontiers Media SA
Published
2016-08-15
Volume
7
Pages
1249-1249
ISSN
1664-302X (ISSN-L)
DOI
10.3389/fmicb.2016.01249
PMC
PMC4983571
OpenAlex
W2513903295
Cited by
74
References
57
Citations by year
2026: 2 · 2025: 7 · 2024: 6 · 2023: 9 · 2022: 7 · 2021: 17 · 2020: 5 · 2019: 9 · 2018: 5 · 2017: 7
FWCI
8.82
Type
journal article
Language
en
Update notices
corrigendum (2016-09-27)
Open access
Licence
cc-by
Locations
  • Frontiers in Microbiology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Padua Research Archive (University of Padova) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Ministero dell'Istruzione, dell'Università e della Ricerca
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Fermentation and Sensory Analysis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Horticultural and Viticultural Research
Keywords
Starmerella bacillaris, Candida zemplinina, Botrytis cinerea, gray mold, biological control, antifungal activity, volatile organic compounds, alcoholic fermentation, wine yeast selection, Saccharomyces cerevisiae, must fermentation, glycerol, ethanol, acetic acid

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