A Perspective Study of Koumiss Microbiome by Metagenomics Analysis Based on Single-Cell Amplification Technique
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Koumiss is a traditional fermented dairy product and a good source for isolating novel bacteria with biotechnology potential. In the present study, we applied the single-cell amplification technique in the metagenomics analysis of koumiss. This approach aimed at detecting the low-abundant bacteria in the koumiss. Briefly, each sample was first serially diluted until reaching the level of approximately 100 cells. Then, three diluted bacterial suspensions were randomly picked for further study. By analyzing 30 diluted koumiss suspensions, a total of 24 bacterial species were identified. In addition to the previously reported koumiss-associated species, such as Lactobacillus (L.) helveticus, Lactococcus lactis, L. buchneri, L. kefiranofaciens and Acetobacter pasteurianus, we successfully detected three low-abundant taxa in the samples, namely L. otakiensis, Streptococcus macedonicus, and Ruminococcus torques. The functional koumiss metagenomes carried putative genes that relate to lactose metabolism and synthesis of typical flavor compounds. Our study would encourage the use of modern metagenomics to discover novel species of bacteria that could be useful in food industries.
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Metagenomic studies have been mainly conducted on single food types and on limited sample sizes, hindering inter-study integrative analyses currently available only for other environments such as the human gut.
“Nevertheless, metagenomic studies have been mainly conducted on single food types 18-24 and on limited sample sizes, 25,26 hindering inter-study integrative analyses currently available only for other environments such as the human gut. 27-31”
From Unexplored microbial diversity from 2,500 food metagenomes and links with the human microbiome. (2024) · cites this paper as [23]
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How genomics can help unravel the evolution of endophytic fungi
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- Authors
- Yao, G., Yu, J., Hou, Q., Hui, W., Liu, W., Kwok, L.-Y., Menghe, B., Sun, T., Zhang, H., & Zhang, W.
- Affiliations
- Inner Mongolia Agricultural University (CN)
- Corresponding author
- Zhang, Wenyi
- ORCID
- Venue
- Frontiers in Microbiology
- Publisher
- Frontiers Media SA
- Published
- 2017-02-07
- Volume
- 8
- Pages
- 165-165
- ISSN
- 1664-302X (ISSN-L)
- PMC
- PMC5293792
- OpenAlex
- W2586424091
- Cited by
- 66
- References
- 43
- Citations by year
- 2026: 7 · 2025: 5 · 2024: 5 · 2023: 7 · 2022: 9 · 2021: 10 · 2020: 8 · 2019: 6 · 2018: 7 · 2017: 2
- FWCI
- 7.08
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Frontiers in Microbiology (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (31571815)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Food Science › Fermentation and Sensory Analysis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Keywords
- koumiss, metagenomics, Lactobacillus helveticus, Lactococcus lactis, Lactobacillus buchneri, Lactobacillus kefiranofaciens, Acetobacter pasteurianus, Streptococcus macedonicus, Ruminococcus torques, lactose metabolism, novel bacterial species
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