Comparative MRNA-Seq Analysis Reveals the Improved EPS Production Machinery in Streptococcus Thermophilus ASCC 1275 during Optimized Milk Fermentation
Open accessAbstract
Exo-polysaccharide (EPS) produced by dairy starters plays critical roles in improving texture and functionalities of fermented dairy products. One of such high EPS producers, Str. thermophilus ASCC 1275 (ST1275) was used as a model dairy strain to understand the stimulation of its EPS production under optimal milk fermentation conditions. The mRNA-seq analysis and targeted pathway analysis indicate that genes associated with lactose (milk sugar) catabolism, EPS assembly, proteolytic activity, and arginine/methionine/cysteine synthesis and transport in ST1275 were significantly up-regulated under the optimized conditions of pH 5.5, 40°C or WPI supplementation compared to that of pH 6.5 and 37°C, respectively. This indicates that genes involved in above metabolisms cooperate together for improving EPS yield from ST1275. This study provides a global view map on potential targeted pathways and specific genes accounted for enhanced EPS production in Str. thermophilus and that could be modulated by fermentation conditions.
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Overexpression of genes related to EPS production was demonstrated in S. thermophilus and Lcb. paracasei when stressing conditions are encountered.
“Other studies demonstrated this overexpression of genes related to EPS production in other species such as S. thermophilus [80] and Lcb. paracasei [81] when stressing conditions are encountered.”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [80]
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Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods
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- Authors
- Wu, Q., & Shah, N. P.
- Affiliations
- University of Hong Kong (HK)
- Corresponding author
- Shah, Nagendra P.
- Venue
- Frontiers in Microbiology
- Publisher
- Frontiers Media SA
- Published
- 2018-03-13
- Volume
- 9
- Pages
- 445-445
- ISSN
- 1664-302X (ISSN-L)
- PMC
- PMC5859087
- OpenAlex
- W2791346556
- Cited by
- 38
- References
- 44
- Citations by year
- 2025: 6 · 2024: 2 · 2023: 4 · 2022: 5 · 2021: 8 · 2020: 8 · 2019: 3 · 2018: 1 · 2015: 1
- FWCI
- 4.69
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Frontiers in Microbiology (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Research Grants Council, University Grants Committee (17105315)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- Streptococcus thermophilus, exopolysaccharide production, dairy fermentation, dairy starter cultures, proteolytic activity, whey protein isolate, fermentation temperature, pH effect, gene expression, fermented dairy products
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