Cited by 38Probiotics and Fermented FoodsPolysaccharides Composition and ApplicationsBacterial Genetics and Biotechnology

Comparative MRNA-Seq Analysis Reveals the Improved EPS Production Machinery in Streptococcus Thermophilus ASCC 1275 during Optimized Milk Fermentation

Open access
Wu, Qinglong, Shah, Nagendra P.·2018·Frontiers in Microbiology

Abstract

Exo-polysaccharide (EPS) produced by dairy starters plays critical roles in improving texture and functionalities of fermented dairy products. One of such high EPS producers, Str. thermophilus ASCC 1275 (ST1275) was used as a model dairy strain to understand the stimulation of its EPS production under optimal milk fermentation conditions. The mRNA-seq analysis and targeted pathway analysis indicate that genes associated with lactose (milk sugar) catabolism, EPS assembly, proteolytic activity, and arginine/methionine/cysteine synthesis and transport in ST1275 were significantly up-regulated under the optimized conditions of pH 5.5, 40°C or WPI supplementation compared to that of pH 6.5 and 37°C, respectively. This indicates that genes involved in above metabolisms cooperate together for improving EPS yield from ST1275. This study provides a global view map on potential targeted pathways and specific genes accounted for enhanced EPS production in Str. thermophilus and that could be modulated by fermentation conditions.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.3389/fmicb.2018.00445

Cites

44 of 44 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Overexpression of genes related to EPS production was demonstrated in S. thermophilus and Lcb. paracasei when stressing conditions are encountered.

    “Other studies demonstrated this overexpression of genes related to EPS production in other species such as S. thermophilus [80] and Lcb. paracasei [81] when stressing conditions are encountered.”

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Wu, Q., & Shah, N. P.
Affiliations
University of Hong Kong (HK)
Corresponding author
Shah, Nagendra P.
Venue
Frontiers in Microbiology
Published
2018-03-13
Volume
9
Pages
445-445
ISSN
1664-302X (ISSN-L)
OpenAlex
W2791346556
Cited by
38
References
44
Citations by year
2025: 6 · 2024: 2 · 2023: 4 · 2022: 5 · 2021: 8 · 2020: 8 · 2019: 3 · 2018: 1 · 2015: 1
FWCI
4.69
Type
journal article
Language
en
Open access
Locations
  • Frontiers in Microbiology (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Research Grants Council, University Grants Committee (17105315)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
Streptococcus thermophilus, exopolysaccharide production, dairy fermentation, dairy starter cultures, proteolytic activity, whey protein isolate, fermentation temperature, pH effect, gene expression, fermented dairy products

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review