Evaluating the capacity of human gut microorganisms to colonize the zebrafish larvae (Danio rerio)
Open accessAbstract
In this study we evaluated if zebrafish larvae can be colonized by human gut microorganisms. We tested two strategies: 1) through transplantation of a human fecal microbiota and 2) by successively transplanting aerotolerant anaerobic microorganisms, similar to the colonization in the human intestine during early life. We used conventionally raised zebrafish larvae harboring their own aerobic microbiota to improve the colonization of anaerobic microorganisms. The results showed with the fecal transplant, that some members of the human gut microbiota were transferred to larvae. Bacillus, Roseburia, Prevotella, Oscillospira, one unclassified genus of the family Ruminococcaceae and Enterobacteriaceae were detected in 3 days post fertilization (dpf) larvae; however only Bacillus persisted to 7 dpf. Successive inoculation of Lactobacillus, Bifidobacterium and Clostridioides did not improve their colonization, compared to individual inoculation of each bacterium. Interestingly, the sporulating bacteria Bacillus clausii and Clostridioides difficile were the most persistent microorganisms. Their endospores persisted at least 5 days after inoculating 3 dpf larvae. However, when 5 dpf larvae were inoculated, the proportion of vegetative cells in larvae increased, revealing proliferation of the inoculated bacteria and better colonization of the host. In conclusion, these results suggest that it is feasible to colonize zebrafish larvae with some human bacteria, such as C. difficile and Bacillus and open an interesting area to study interactions between these microorganisms and the host.
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Relying solely on high-throughput sequencing to analyze the makeup of gut bacteria is insufficient to conclusively determine whether Lactobacillus can effectively colonize the intestines and exert its beneficial effects.
“To fully comprehend its significance, it is crucial to acknowledge that relying solely on high-throughput sequencing to analyze the makeup of gut bacteria is insufficient to conclusively determine whether Lactobacillus can effectively colonize the intestines and exert its beneficial effects (Valenzuela et al., 2018).”
From Lactiplantibacillus plantarum N4 ameliorates lipid metabolism and gut microbiota structure in high fat diet-fed rats (2024) · cites this paper as Valenzuela et al., 2018
The Results (page unavailable) say direct MiSeq sequencing and culture-based analysis were complementary, and report that persistence in larvae was checked by culture when bacterial DNA could not be amplified for sequencing. The paper also measures Lactobacillus persistence in zebrafish larvae, but it does not assess whether Lactobacillus exerts beneficial effects; that part of the claim is unsupported. (LLM, conf=0.86)
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Deng, M., Zhang, S., Wu, S., Jiang, Q., Teng, W., Luo, T., Ouyang, Y., Liu, J., & Gu, B. (2024) · Frontiers in Microbiology · doi:10.3389/fmicb.2024.1390293 · Cited by 15 · see citing works
- Authors
- Valenzuela, M.-J., Caruffo, M., Herrera, Y., Medina, D. A., Coronado, M., Feijóo, C. G., Muñoz, S., Garrido, D., Troncoso, M., Figueroa, G., Toro, M., Reyes-Jara, A., Magne, F., & Navarrete, P.
- Affiliations
- University of Chile (CL)
- University of Michigan (US)
- Pontificia Universidad Católica de Chile (CL)
- Universidad Andrés Bello (CL)
- Corresponding author
- Magne, Fabien
- Navarrete, Paola
- ORCID
- Venue
- Frontiers in Microbiology
- Publisher
- Frontiers Media SA
- Published
- 2018-05-29
- Volume
- 9
- Pages
- 1032-1032
- ISSN
- 1664-302X (ISSN-L)
- PMC
- PMC5987363
- OpenAlex
- W2799762643
- Cited by
- 39
- References
- 53
- Citations by year
- 2026: 6 · 2025: 3 · 2024: 5 · 2023: 6 · 2022: 3 · 2021: 8 · 2020: 2 · 2019: 5 · 2018: 1
- FWCI
- 1.42
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Frontiers in Microbiology (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Universidad de Chile (submittedVersion, cc-by-nc-nd)
- Repositorio Institucional Académico , Sistema de Bibliotecas Universidad Andrés Bello (UNAB) (Universidad Andrés Bello (UNAB)) (submittedVersion)
- Funders
- University of Michigan
- Universidad de Chile
- National Institutes of Health
- University of Illinois at Urbana-Champaign
- Comisión Nacional de Investigación Científica y Tecnológica (3160525)
- National Institute on Minority Health and Health Disparities (T37MD001425)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Immunology and Microbiology › Immunology › Aquaculture disease management and microbiota
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
- Keywords
- zebrafish larvae, human gut microbiome, bacterial colonization, fecal microbiota transplantation, anaerobic bacteria, Bacillus clausii, Clostridium difficile, endospores, Lactobacillus, Bifidobacterium, early life colonization, host-microbe interactions, gut microbiota
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