Surfactin Facilitates Horizontal Gene Transfer in Bacillus subtilis
Open accessAbstract
Genetic competence for the uptake and integration of extracellular DNA is a key process in horizontal gene transfer (HGT), one of the most powerful forces driving the evolution of bacteria. In several species, development of genetic competence is coupled with cell lysis. Using Bacillus subtilis as a model bacterium, we studied the role of surfactin, a powerful biosurfactant and antimicrobial lipopeptide, in genetic transformation. We showed that surfactin itself promotes cell lysis and DNA release, thereby promoting HGT. These results, therefore, provide evidence for a fundamental mechanism involved in HGT and significantly increase our understanding of the spreading of antibiotic resistance genes and diversification of microbial communities in the environment.
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Bacillus HGT can also occur via other mechanisms, including transformation-mediated processes.
“Indeed, Bacillus HGT can also occur via other mechanisms, including transformation-mediated processes [96, 104, 105].”
From Profiling the resistome and virulome of Bacillus strains used for probiotic-based sanitation: a multicenter WGS analysis (2025) · cites this paper as [104]
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- Authors
- Danevčič, T., Dragoš, A., Spacapan, M., Stefanic, P., Dogsa, I., & Mandic-Mulec, I.
- Affiliations
- University of Ljubljana (SI)
- Corresponding author
- Danevčič, Tjaša
- Venue
- Frontiers in Microbiology
- Publisher
- Frontiers Media SA
- Published
- 2021-05-14
- Volume
- 12
- Pages
- 657407-657407
- ISSN
- 1664-302X (ISSN-L)
- PMC
- PMC8160284
- OpenAlex
- W3160334367
- Cited by
- 35
- References
- 50
- Citations by year
- 2026: 5 · 2025: 7 · 2024: 10 · 2023: 5 · 2022: 6 · 2021: 2
- FWCI
- 3.25
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Frontiers in Microbiology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Repository of the University of Ljubljana (University of Ljubljana) (publishedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Technical University of Denmark, DTU Orbit (Technical University of Denmark, DTU) (submittedVersion, cc-by)
- Funders
- Javna Agencija za Raziskovalno Dejavnost RS (P4-0116, J4-8228, J4-9302, J4-1175)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Physical Sciences › Environmental Science › Pollution › Microbial bioremediation and biosurfactants
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
- Keywords
- surfactin, horizontal gene transfer, Bacillus subtilis, genetic competence, cell lysis, DNA release, genetic transformation, biosurfactants, lipopeptides, antibiotic resistance genes
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