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Cited by 62Pharmaceutical and Antibiotic Environmental ImpactsMicrobial bioremediation and biosurfactantsSteroid Chemistry and Biochemistry

Rhodococcus strains as a good biotool for neutralizing pharmaceutical pollutants and obtaining therapeutically valuable products: Through the past into the future

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Ivshina, Irina, Bazhutin, Grigory, Tyumina, Elena·2022·Frontiers in Microbiology
DOI 10.3389/fmicb.2022.967127PMC PMC9557007
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Abstract

Active pharmaceutical ingredients present a substantial risk when they reach the environment and drinking water sources. As a new type of dangerous pollutants with high chemical resistance and pronounced biological effects, they accumulate everywhere, often in significant concentrations (μg/L) in ecological environments, food chains, organs of farm animals and humans, and cause an intense response from the aquatic and soil microbiota. Rhodococcus spp. ( Actinomycetia class), which occupy a dominant position in polluted ecosystems, stand out among other microorganisms with the greatest variety of degradable pollutants and participate in natural attenuation, are considered as active agents with high transforming and degrading impacts on pharmaceutical compounds. Many representatives of rhodococci are promising as unique sources of specific transforming enzymes, quorum quenching tools, natural products and novel antimicrobials, biosurfactants and nanostructures. The review presents the latest knowledge and current trends regarding the use of Rhodococcus spp. in the processes of pharmaceutical pollutants’ biodegradation, as well as in the fields of biocatalysis and biotechnology for the production of targeted pharmaceutical products. The current literature sources presented in the review can be helpful in future research programs aimed at promoting Rhodococcus spp. as potential biodegraders and biotransformers to control pharmaceutical pollution in the environment.

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  • The genus Rhodococcus was quickly recognized as one of the most attractive groups of organisms for the removal of chemicals that are not readily converted by other species.

    “The genus Rhodococcus was quickly recognized as one of the most attractive groups of organisms for the removal of chemicals that are not readily converted by other species [161].”

    From Harnessing bacterial consortia for effective bioremediation: targeted removal of heavy metals, hydrocarbons, and persistent pollutants (2025) · cites this paper as [161]

    The Introduction describes Rhodococcus as capable of degrading recalcitrant pollutants and calls it a promising biotechnology tool; the Biodegradation of pharmaceutical pollutants section also highlights its exceptional degrading abilities. These passages support its appeal for removing difficult-to-degrade chemicals, but do not establish that it was quickly recognized as such or directly compare its abilities with those of other species. No page numbers are available in our copy. (LLM, conf=0.82)

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  1. Abreu, 2018, Estradiol shapes mutualistic behaviour of female cleaner fish (Labroides dimidiatus - Valenciennes, 1839): potential implications of environmental disturbance, Ecotoxicol. Environ. Saf., 157, 244, 10.1016/J.ECOENV.2018.03.072 · DOI
  2. Afshinnekoo, 2021, COVID-19 drug practices risk antimicrobial resistance evolution, Lancet Microbe, 2, e135, 10.1016/S2666-5247(21)00039-2 · DOI
  3. Aguilar-Romero, 2020, Bacterial ecotoxicity and shifts in bacterial communities associated with the removal of ibuprofen, diclofenac and triclosan in biopurification systems, Sci. Total Environ., 741, 140461, 10.1016/J.SCITOTENV.2020.140461 · DOI
  4. Akay, 2020, Biotransformation of acetaminophen by intact cells and crude enzymes of bacteria: a comparative study and modelling, Sci. Total Environ., 703, 134990, 10.1016/j.scitotenv.2019.134990 · DOI
  5. Alam, 2021, Silver nanoparticles biosynthesized from secondary metabolite producing marine actinobacteria and evaluation of their biomedical potential, Antonie Van Leeuwenhoek, 114, 1497, 10.1007/S10482-021-01616-5 · DOI
  6. Alvarez, 2021, Rhodococcus as biofactories for microbial oil production, Molecules, 26, 4871, 10.3390/MOLECULES26164871 · DOI
  7. Anissi, 2021, Production of hydroxytyrosol from hydroxylation of tyrosol by Rhodococcus pyridinivorans 3HYL DSM109178, Biocatal. Biotransformation, 39, 418, 10.1080/10242422.2021.1903884 · DOI
  8. Annamalai, 2020, Enhanced biodegradation of an endocrine disrupting micro-pollutant: Di (2-ethylhexyl) phthalate using biogenic self-assembled monolayer of silver nanoparticles, Sci. Total Environ., 719, 137115, 10.1016/J.SCITOTENV.2020.137115 · DOI
  9. Anteneh, 2019, Whole cell Actinobacteria as biocatalysts, Front. Microbiol., 10, 77, 10.3389/FMICB.2019.00077 · DOI
  10. Anyanwu, 2021, Oxidation of cadaverine by putrescine oxidase from Rhodococcus erythropolis, J. Chem. Technol. Biotechnol., 96, 2950, 10.1002/JCTB.6851 · DOI
  11. aus der Beek, 2016, Pharmaceuticals in the environment–global occurrences and perspectives, Environ. Toxicol. Chem., 35, 823, 10.1002/etc.3339 · DOI · Paper page
  12. Auta, 2018, Growth kinetics and biodeterioration of polypropylene microplastics by bacillus sp. and Rhodococcus sp. isolated from mangrove sediment, Mar. Pollut. Bull., 127, 15, 10.1016/j.marpolbul.2017.11.036 · DOI · Paper page
  13. Baek, 2022, Elucidating the biodegradation pathway and catabolic genes of benzophenone-3 in Rhodococcus sp. S2-17, Environ. Pollut., 299, 118890, 10.1016/J.ENVPOL.2022.118890 · DOI
  14. Baldanta, 2021, Further studies on the 3-ketosteroid 9α-hydroxylase of Rhodococcus ruber Chol-4, a Rieske oxygenase of the steroid degradation pathway, Microorganisms, 9, 1171, 10.3390/MICROORGANISMS9061171 · DOI
  15. Barbey, 2018, A rhodococcal transcriptional regulatory mechanism detects the common lactone ring of AHL quorum-sensing signals and triggers the quorum-quenching response, Front. Microbiol., 9, 2800, 10.3389/FMICB.2018.02800 · DOI
  16. Barra Caracciolo, 2015, Pharmaceuticals in the environment: biodegradation and effects on natural microbial communities. A review, J. Pharm. Biomed. Anal., 106, 25, 10.1016/j.jpba.2014.11.040 · DOI
  17. Bazhutin, 2022, Cometabolic bioconversion of ketoprofen by Rhodococcus erythropolis IEGM 746, 404, 10.1007/978-3-030-89477-1_40 · DOI
  18. Bębenek, 2018, Novel triazoles of 3-acetylbetulin and betulone as anticancer agents, Med. Chem. Res., 27, 2051, 10.1007/S00044-018-2213-X · DOI
  19. Bej, 2000, Cold-tolerant alkane-degrading Rhodococcus species from Antarctica, Polar Biol., 23, 100, 10.1007/s003000050014 · DOI
  20. Billot, 2020, Engineering acyl-homoserine lactone-interfering enzymes toward bacterial control, J. Biol. Chem., 295, 12993, 10.1074/JBC.REV120.013531 · DOI
  21. Bode, 2002, Big effects from small changes: possible ways to explore nature’s chemical diversity, Chem BioChem, 3, 619, 10.1002/1439-7633(20020703)3:7<619::AID-CBIC619>3.0.CO;2-9 · DOI
  22. Bosello, 2011, Biosynthesis of the siderophore rhodochelin requires the coordinated expression of three independent gene clusters in Rhodococcus jostii RHA1, J. Am. Chem. Soc., 133, 4587, 10.1021/JA1109453 · DOI
  23. Bouju, 2012, Isolation of bacterial strains capable of sulfamethoxazole mineralization from an acclimated membrane bioreactor, Appl. Environ. Microbiol., 78, 277, 10.1128/AEM.05888-11 · DOI
  24. Bourigault, 2021, Biocontrol of biofilm formation: jamming of sessile-associated rhizobial communication by rhodococcal quorum-quenching, Int. J. Mol. Sci., 22, 8241, 10.3390/IJMS22158241 · DOI
  25. Busch, 2019, Rhodococcus as a versatile biocatalyst in organic synthesis, Int. J. Mol. Sci., 20, 4787, 10.3390/IJMS20194787 · DOI
  26. Cappelletti, 2016, Phenotype microarray analysis may unravel genetic determinants of the stress response by Rhodococcus aetherivorans BCP1 and Rhodococcus opacus R7, Res. Microbiol., 167, 766, 10.1016/J.RESMIC.2016.06.008 · DOI
  27. Cappelletti, 2020, Biotechnology of Rhodococcus for the production of valuable compounds, Appl. Microbiol. Biotechnol., 104, 8567, 10.1007/s00253-020-10861-z · DOI
  28. Cappelletti, 2019, Genomics of Rhodococcus, 23, 10.1007/978-3-030-11461-9_2 · DOI
  29. Cappelli, 2022, Suspect screening of wastewaters to trace anti-COVID-19 drugs: potential adverse effects on aquatic environment, Sci. Total Environ., 824, 153756, 10.1016/J.SCITOTENV.2022.153756 · DOI
  30. Carpova, 2011, Transformation of Δ4-3-ketosteroids by free and immobilized cells of Rhodococcus erythropolis actinobacterium, Appl. Biochem. Microbiol., 47, 386, 10.1134/s0003683811040041 · DOI
  31. Ceniceros, 2017, Genome-based exploration of the specialized metabolic capacities of the genus Rhodococcus, BMC Genomics, 18, 593, 10.1186/S12864-017-3966-1 · DOI
  32. Chane, 2020, Close-up on a bacterial informational war in the geocaulosphere, Can. J. Microbiol., 66, 447, 10.1139/CJM-2019-0546 · DOI
  33. Chartrain, 1998, Bioconversion of indene to cis (1S,2R) indandiol and trans (1R,2R) indandiol by Rhodococcus species, J. Ferment. Bioeng., 86, 550, 10.1016/S0922-338X(99)80005-1 · DOI
  34. Chen, 2020, Using choline chloride-based dess as co-solvent for 3,5-bis(trifluoromethyl) acetophenone bioreduction with Rhodococcus erythropolis XS 1012, Catalysts, 10, 30, 10.3390/catal10010030 · DOI
  35. Chen, 2014, Integrated omics study delineates the dynamics of lipid droplets in Rhodococcus opacus PD630, Nucleic Acids Res., 42, 1052, 10.1093/NAR/GKT932 · DOI
  36. Chevrette, 2020, Evolutionary dynamics of natural product biosynthesis in bacteria, Nat. Prod. Rep., 37, 566, 10.1039/C9NP00048H · DOI
  37. Chiba, 1999, Rhodopeptins (Mer-N1033), novel cyclic tetrapeptides with antifungal activity from Rhodococcus sp. I. Taxonomy, fermentation, isolation, physico-chemical properties and biological activities, J. Antibiot., 52, 695, 10.7164/ANTIBIOTICS.52.695 · DOI
  38. Choi, 2019, Cyanide-free synthesis of an aromatic nitrile from a biorenewable-based aldoxime: development and application of a recombinant aldoxime dehydratase as a biocatalyst, Biocatal. Biotransformation, 37, 414, 10.1080/10242422.2019.1591376 · DOI
  39. Chopra, 2001, Tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology of bacterial resistance, Microbiol. Mol. Biol. Rev., 65, 232, 10.1128/MMBR.65.2.232-260.2001 · DOI · Paper page
  40. Chu, 2016, Discovery of MRSA active antibiotics using primary sequence from the human microbiome, Nat. Chem. Biol., 12, 1004, 10.1038/nchembio.2207 · DOI
  41. Çobanoğlu, 2021, Isolation, characterization, and antibiofilm activity of pigments synthesized by Rhodococcus sp. SC1, Curr. Microbiol., 79, 15, 10.1007/S00284-021-02694-4 · DOI
  42. Coleman, 2002, Evidence that RDX biodegradation by Rhodococcus strain DN22 is plasmid-borne and involves a cytochrome p-450, J. Appl. Microbiol., 93, 463, 10.1046/j.1365-2672.2002.01713.x · DOI
  43. Costa, 2013, New 9,10-secosteroids from biotransformations of bile acids with Rhodococcus ruber, Helv. Chim. Acta, 96, 2124, 10.1002/HLCA.201300114 · DOI
  44. Costa, 2020, Δ1-dehydrogenation and C20 reduction of cortisone and hydrocortisone catalyzed by Rhodococcus strains, Molecules, 25, 2192, 10.3390/MOLECULES25092192 · DOI
  45. Crampon, 2021, Dynamics of soil microbial communities during diazepam and oxazepam biodegradation in soil flooded by water from a WWTP, Front. Microbiol., 12, 742000, 10.3389/FMICB.2021.742000 · DOI
  46. Cycoń, 2019, Antibiotics in the soil environment – degradation and their impact on microbial activity and diversity, Front. Microbiol., 10, 338, 10.3389/fmicb.2019.00338 · DOI
  47. Dai, Rational regulation of reaction specificity of nitrilase for efficient biosynthesis of 2-chloronicotinic acid through a single site mutation, Appl. Environ. Microbiol., 88, aem.02397-21, 10.1128/aem.02397-21 · DOI
  48. Dai, Constitutive expression of nitrilase from Rhodococcus zopfii for efficient biosynthesis of 2-chloronicotinic acid. 3, Biotech, 12, 50, 10.1007/S13205-022-03119-0 · DOI
  49. Daniel, 2022, Toxicity of two drugs towards the marine filter feeder Mytilus spp, using biochemical and shell integrity parameters, Environ. Pollut., 293, 118562, 10.1016/J.ENVPOL.2021.118562 · DOI
  50. Darme, 2022, Investigation of antiparasitic activity of 10 European tree bark extracts on toxoplasma gondii and bioguided identification of triterpenes in Alnus glutinosa barks, Antimicrob. Agents Chemother., 66, e01098, 10.1128/AAC.01098-21 · DOI
  51. Dasenaki, 2015, Multianalyte method for the determination of pharmaceuticals in wastewater samples using solid-phase extraction and liquid chromatography-tandem mass spectrometry, Anal. Bioanal. Chem., 407, 4229, 10.1007/s00216-015-8654-x · DOI
  52. Daughton, 2014, Green pharmacy and pharmEcovigilance: prescribing and the planet, Expert. Rev. Clin. Pharmacol., 4, 211, 10.1586/ECP.11.6 · DOI
  53. de Carvalho, 2017, Whole cell biocatalysts: essential workers from nature to the industry, Microb. Biotechnol., 10, 250, 10.1111/1751-7915.12363 · DOI
  54. de Carvalho, 2014, Membrane transport systems and the biodegradation potential and pathogenicity of genus Rhodococcus, Front. Physiol., 5, 133, 10.3389/fphys.2014.00133 · DOI
  55. DeLorenzo, 2021, An improved CRISPR interference tool to engineer Rhodococcus opacus, ACS Synth. Biol., 10, 786, 10.1021/acssynbio.0c00591 · DOI
  56. Devine, 2018, Extending the application of biocatalysis to meet the challenges of drug development, Nat. Rev. Chem., 2, 409, 10.1038/s41570-018-0055-1 · DOI
  57. Dhoke, 2020, Reversal of regioselectivity in zinc-dependent medium-chain alcohol dehydrogenase from Rhodococcus erythropolis toward octanone derivatives, Chembiochem, 21, 2957, 10.1002/CBIC.202000247 · DOI
  58. di Canito, 2018, Genome-based analysis for the identification of genes involved in o-xylene degradation in Rhodococcus opacus R7, BMC Genom., 19, 587, 10.1186/s12864-018-4965-6 · DOI
  59. Domingo-Echaburu, 2021, Drug pollution & sustainable development goals, Sci. Total Environ., 800, 149412, 10.1016/J.SCITOTENV.2021.149412 · DOI
  60. Dong, 2018, Biocatalytic oxidation reactions: a chemist’s perspective, Angew. Chem. Int. Ed., 57, 9238, 10.1002/ANIE.201800343 · DOI
  61. Donini, 2021, Systems biology and metabolic engineering of Rhodococcus for bioconversion and biosynthesis processes, Folia Microbiol., 2021, 701, 10.1007/S12223-021-00892-Y · DOI
  62. Donova, 2017, Steroid bioconversions, 1, 10.1007/978-1-4939-7183-1_1 · DOI
  63. Drzymała, 2020, Ecotoxic interactions between pharmaceuticals in mixtures: Diclofenac and sulfamethoxazole, Chemosphere, 259, 127407, 10.1016/j.chemosphere.2020.127407 · DOI
  64. Duan, 2022, Occurrence and ecotoxicity of sulfonamides in the aquatic environment: a review, Sci. Total Environ., 820, 153178, 10.1016/J.SCITOTENV.2022.153178 · DOI
  65. Duarte, 2020, Effects of propranolol on growth, lipids and energy metabolism and oxidative stress response of Phaeodactylum tricornutum, Biology, 9, 478, 10.3390/BIOLOGY9120478 · DOI
  66. Effenberger, 1997, Preparation of (S)-naproxen by enantioselective hydrolysis of racemic naproxen amide with resting cells of Rhodococcus erythropolis MP50 in organic solvents, Tetrahedron Asymmetry, 8, 2749, 10.1016/S0957-4166(97)00335-2 · DOI
  67. Esposito, 2021, Combining OSMAC approach and untargeted metabolomics for the identification of new glycolipids with potent antiviral activity produced by a marine Rhodococcus, Int. J. Mol. Sci., 22, 9055, 10.3390/IJMS22169055 · DOI
  68. Felpeto-Santero, 2021, Production of 11α-hydroxysteroids from sterols in a single fermentation step by Mycolicibacterium smegmatis, Microb. Biotechnol., 14, 2514, 10.1111/1751-7915.13735 · DOI
  69. Feng, 2019, Co-metabolic degradation of the antibiotic ciprofloxacin by the enriched bacterial consortium XG and its bacterial community composition, Sci. Total Environ., 665, 41, 10.1016/J.SCITOTENV.2019.01.322 · DOI
  70. Fernández de las Heras, 2009, Morphological, physiological, and molecular characterization of a newly isolated steroid-degrading actinomycete, identified as Rhodococcus ruber strain Chol-4, Curr. Microbiol., 59, 548, 10.1007/s00284-009-9474-z · DOI
  71. Fernández de las Heras, 2011, ChoG is the main inducible extracellular cholesterol oxidase of Rhodococcus sp. strain CECT 3014, Microbiol. Res., 166, 403, 10.1016/J.MICRES.2010.05.003 · DOI
  72. Fernández de las Heras, 2014, Cholesterol to cholestenone oxidation by ChoG, the main extracellular cholesterol oxidase of Rhodococcus ruber strain Chol-4, J. Steroid Biochem. Mol. Biol., 139, 33, 10.1016/J.JSBMB.2013.10.001 · DOI
  73. Fernández de las Heras, 2012, Molecular characterization of three 3-ketosteroid-Δ1- dehydrogenase isoenzymes of Rhodococcus ruber strain Chol-4, J. Steroid Biochem. Mol. Biol., 132, 271, 10.1016/j.jsbmb.2012.06.005 · DOI
  74. Fernández-Cabezón, 2018, New insights on steroid biotechnology, Front. Microbiol., 9, 958, 10.3389/fmicb.2018.00958 · DOI
  75. Firrincieli, 2019, Identification of resistance genes and response to arsenic in Rhodococcus aetherivorans BCP1, Front. Microbiol., 10, 888, 10.3389/FMICB.2019.00888 · DOI
  76. Firrincieli, 2022, Transcriptomic analysis of the dual response of Rhodococcus aetherivorans BCP1 to inorganic arsenic oxyanions, Appl. Environ. Microbiol., 88, e02209, 10.1128/AEM.02209-21 · DOI
  77. Gamarra, 2015, Environmental risk assessment (ERA) of diclofenac and ibuprofen: a public health perspective, Chemosphere, 120, 462, 10.1016/j.chemosphere.2014.08.020 · DOI
  78. Gauthier, 2010, Biodegradation of pharmaceuticals by Rhodococcus rhodochrous and Aspergillus Niger by co-metabolism, Sci. Total Environ., 408, 1701, 10.1016/j.scitotenv.2009.12.012 · DOI
  79. Gilan, 2013, Effect of proteases on biofilm formation of the plastic-degrading actinomycete Rhodococcus ruber C208, FEMS Microbiol. Lett., 342, 18, 10.1111/1574-6968.12114 · DOI
  80. Giménez, 2019, Effects of commonly used therapeutic drugs, paracetamol, and acetylsalicylic acid, on key physiological traits of the sea snail Gibbula umbilicalis, Environ. Sci. Pollut. Res., 26, 21858, 10.1007/S11356-019-04653-w · DOI
  81. Githinji, 2011, Evaluation of the fate of ciprofloxacin and amoxicillin in domestic wastewater, Water Air Soil Pollut., 219, 191, 10.1007/s11270-010-0697-1 · DOI
  82. Gomes, 2020, Emerging contaminants affect the microbiome of water systems—strategies for their mitigation, NPJ Clean Water, 3, 39, 10.1038/s41545-020-00086-y · DOI
  83. González-Benítez, 2021, Endophytic microbes are tools to increase tolerance in Jasione plants against arsenic stress, Front. Microbiol., 12, 664271, 10.3389/fmicb.2021.664271 · DOI
  84. Goordial, 2016, Cold adaptive traits revealed by comparative genomic analysis of the eurypsychrophile Rhodococcus sp. JG3 isolated from high elevation McMurdo Dry Valley permafrost, Antarctica, FEMS Microbiol. Ecol., 92, fiv154, 10.1093/femsec/fiv154 · DOI
  85. Grabarczyk, 2020, Ecotoxicity screening evaluation of selected pharmaceuticals and their transformation products towards various organisms, Environ. Sci. Pollut. Res., 27, 26103, 10.1007/S11356-020-08881-3 · DOI
  86. Grandclément, 2016, Quorum quenching: role in nature and applied developments, FEMS Microbiol. Rev., 40, 86, 10.1093/FEMSRE/FUV038 · DOI
  87. Gren, 2021, Characterization and engineering of Streptomyces griseofuscus DSM 40191 as a potential host for heterologous expression of biosynthetic gene clusters, Sci. Rep., 11, 18301, 10.1038/s41598-021-97571-2 · DOI
  88. Grenni, 2018, Ecological effects of antibiotics on natural ecosystems: a review, Microchem. J., 136, 25, 10.1016/j.microc.2017.02.006 · DOI
  89. Grishko, 2013, Biotransformation of betulin to betulone by growing and resting cells of the actinobacterium Rhodococcus rhodochrous IEGM 66, Process Biochem., 48, 1640, 10.1016/j.procbio.2013.08.012 · DOI
  90. Guevara, New insights into the genome of Rhodococcus ruber strain Chol-4, BMC Genom., 20, 332, 10.1186/s12864-019-5677-2 · DOI · Paper page
  91. Guevara, Metabolic engineering of Rhodococcus ruber Chol-4: a cell factory for testosterone production, PLoS One, 14, e0220492, 10.1371/JOURNAL.PONE.0220492 · DOI
  92. Guevara, 2017, Functional characterization of 3-ketosteroid 9α-hydroxylases in Rhodococcus ruber strain chol-4, J. Steroid Biochem. Mol. Biol., 172, 176, 10.1016/J.JSBMB.2017.06.011 · DOI
  93. Güneş, 2022, Quorum quenching strategy for biofouling control in membrane photobioreactor, Chemosphere, 288, 132667, 10.1016/J.CHEMOSPHERE.2021.132667 · DOI
  94. Hamamura, 2006, Microbial population dynamics associated with crude-oil biodegradation in diverse soils, Appl. Environ. Microbiol., 72, 6316, 10.1128/AEM.01015-06 · DOI
  95. Han, 2021, Green and scalable synthesis of chiral aromatic alcohols through an efficient biocatalytic system, Microb. Biotechnol., 14, 444, 10.1111/1751-7915.13602 · DOI
  96. Hanson, 2005, Purification and cloning of a ketoreductase used for the preparation of chiral alcohols, Adv. Synth. Catal., 347, 1073, 10.1002/ADSC.200505045 · DOI
  97. Harthern-Flint, 2021, Experimental and genomic evaluation of the oestrogen degrading bacterium Rhodococcus equi ATCC13557, Front. Microbiol., 12, 670928, 10.3389/FMICB.2021.670928 · DOI
  98. Harunari, 2022, Rausuquinone, a non-glycosylated pluramycin-class antibiotic from Rhodococcus, J. Antibiot., 75, 86, 10.1038/s41429-021-00489-0 · DOI
  99. Haußmann, 2013, Physiological adaptation of the Rhodococcus jostii RHA1 membrane proteome to steroids as growth substrates, J. Proteome Res., 12, 1188, 10.1021/pr300816n · DOI
  100. Heilbronner, 2021, The microbiome-shaping roles of bacteriocins, Nat. Rev. Microbiol., 9, 726, 10.1038/s41579-021-00569-w · DOI
  101. Henson, 2018, Multi-omic elucidation of aromatic catabolism in adaptively evolved Rhodococcus opacus, Metab. Eng., 49, 69, 10.1016/J.YMBEN.2018.06.009 · DOI
  102. Hibi, 2021, A three-component monooxygenase from Rhodococcus wratislaviensis may expand industrial applications of bacterial enzymes, Commun. Biol., 4, 16, 10.1038/s42003-020-01555-3 · DOI
  103. Hirrlinger, 1996, Purification and properties of an amidase from Rhodococcus erythropolis MP50 which enantioselectively hydrolyzes 2-arylpropionamides, J. Bacteriol., 178, 3501, 10.1128/JB.178.12.3501-3507.1996 · DOI
  104. Hirschler, 2021, Biodesulfurization induces reprogramming of sulfur metabolism in Rhodococcus qingshengii IGTS8: proteomics and untargeted metabolomics, Microbiol. Spectr., 9, e00692, 10.1128/spectrum.00692-21 · DOI
  105. Holert, 2020, Steryl ester formation and accumulation in steroid-degrading bacteria, Appl. Environ. Microbiol., 86, e02353, 10.1128/AEM.02353-19 · DOI
  106. Honda, 2019, Expression of engineered carbonyl reductase from Ogataea minuta in Rhodococcus opacus and its application to whole-cell bioconversion in anhydrous solvents, J. Biosci. Bioeng., 127, 145, 10.1016/j.jbiosc.2018.07.011 · DOI
  107. Horie, 2019, The non-steroidal anti-inflammatory drug diclofenac sodium induces abnormal embryogenesis and delayed lethal effects in early life stage zebrafish (Danio rerio), J. Appl. Toxicol., 39, 622, 10.1002/jat.3752 · DOI
  108. Hsiao, 2021, Mechanistic and phylogenetic insights into actinobacteria-mediated oestrogen biodegradation in urban estuarine sediments, Microb. Biotechnol., 14, 1212, 10.1111/1751-7915.13798 · DOI
  109. Hu, 2020, Stereoselective synthesis of the key intermediate of ticagrelor and its diverse analogs using a new alcohol dehydrogenase from Rhodococcus kyotonensis, Process Biochem., 92, 232, 10.1016/j.procbio.2020.01.015 · DOI
  110. Huang, 2020, Thiamine-mediated cooperation between auxotrophic Rhodococcus ruber ZM07 and Escherichia coli K12 drives efficient tetrahydrofuran degradation, Front. Microbiol., 11, 594052, 10.3389/fmicb.2020.594052 · DOI
  111. Iqbal, 2018, Effective quorum quenching bacteria dose for anti-fouling strategy in membrane bioreactors utilizing fixed-sheet media, J. Membr. Sci., 562, 18, 10.1016/J.MEMSCI.2018.05.031 · DOI
  112. Ivshina, Responses to ecopollutants and pathogenization risks of saprotrophic Rhodococcus species, Pathogens, 10, 974, 10.3390/PATHOGENS10080974 · DOI · Paper page
  113. Ivshina, 2015, Drotaverine hydrochloride degradation using cyst-like dormant cells of Rhodococcus ruber, Curr. Microbiol., 70, 307, 10.1007/s00284-014-0718-1 · DOI
  114. Ivshina, 2006, Catalysis of the biodegradation of unusable medicines by alkanotrophic rhodococci, Appl. Biochem. Microbiol., 42, 392, 10.1134/S0003683806040090 · DOI
  115. Ivshina, Response of Rhodococcus cerastii IEGM 1278 to toxic effects of ibuprofen, PLoS One, 16, e0260032, 10.1371/JOURNAL.PONE.0260032 · DOI
  116. Ivshina, 2019, Features of diclofenac biodegradation by Rhodococcus ruber IEGM 346, Sci. Rep., 9, 9159, 10.1038/s41598-019-45732-9 · DOI · Paper page
  117. Ivshina, 2012, Biodegradation of drotaverine hydrochloride by free and immobilized cells of Rhodococcus rhodochrous IEGM 608, World J. Microbiol. Biotechnol., 28, 2997, 10.1007/s11274-012-1110-6 · DOI
  118. Ivshina, 2022, Cellular modifications of rhodococci exposed to separate and combined effects of pharmaceutical pollutants, Microorganisms, 10, 1101, 10.3390/MICROORGANISMS10061101 · DOI
  119. Iwatsuki, 2006, Lariatins, antimycobacterial peptides produced by Rhodococcus sp. K01-B 0171, have a lasso structure, J. Am. Chem. Soc., 128, 7486, 10.1021/JA056780Z · DOI
  120. Izadi, 2020, Non-steroidal anti-inflammatory drugs in the environment: where were we and how far we have come?, Environ. Pollut., 267, 115370, 10.1016/j.envpol.2020.115370 · DOI
  121. Jang, 2022, Applications of artificial intelligence to enzyme and pathway design for metabolic engineering, Curr. Opin. Biotechnol., 73, 101, 10.1016/J.COPBIO.2021.07.024 · DOI
  122. Jia, Actinobacterial communities of chosen extreme habitats in China, Pol. J. Ecol., 68, 181, 10.3161/15052249PJE2020.68.3.001 · DOI
  123. Jia, Alterations in the cell wall of Rhodococcus biphenylivorans under norfloxacin stress, Front. Microbiol., 11, 554957, 10.3389/fmicb.2020.554957 · DOI
  124. Jiang, 2017, Impact of selected non-steroidal anti-inflammatory pharmaceuticals on microbial community assembly and activity in sequencing batch reactors, PLoS One, 12, e0179236, 10.1371/journal.pone.0179236 · DOI
  125. Jiang, 2020, Synthesizing chiral drug intermediates by biocatalysis, Appl. Biochem. Biotechnol., 192, 146, 10.1007/S12010-020-03272-3 · DOI
  126. Jones, 2015, Rhodococcus, 1, 10.1002/9781118960608.gbm00033 · DOI
  127. Junaid, 2022, Antibiotics and antibiotic resistant genes in urban aquifers, Curr. Opin. Environ. Sci. Health, 26, 100324, 10.1016/J.COESH.2021.100324 · DOI
  128. Kalia, 2019, Quorum sensing inhibitors as antipathogens: biotechnological applications, Biotechnol. Adv., 37, 68, 10.1016/J.BIOTECHADV.2018.11.006 · DOI
  129. Kamaraj, 2022, Biodegradation of di-(2-ethylhexyl) phthalate by novel Rhodococcus sp. PFS1 strain isolated from paddy field soil, Arch. Microbiol., 204, 21, 10.1007/S00203-021-02632-9 · DOI
  130. Keasling, 2021, Microbial production of advanced biofuels, Nat. Rev. Microbiol., 19, 701, 10.1038/s41579-021-00577-w · DOI
  131. Khrenkov, 2020, Chromatographic analysis of acetylsalicylic acid in Rhodococcus cultural fluids, Mosc. Univ. Chem. Bull., 75, 309, 10.3103/S0027131420050053 · DOI
  132. Kim, 2021, Machine learning applications in genome-scale metabolic modeling, Curr. Opin. Syst. Biol., 25, 42, 10.1016/J.COISB.2021.03.001 · DOI
  133. Kim, 2007, Steroid 9α-hydroxylation during testosterone degradation by resting Rhodococcus equi cells, Arch Pharm., 340, 209, 10.1002/ardp.200600175 · DOI
  134. Kitagawa, 2008, A Quinoline antibiotic from Rhodococcus erythropolis JCM 6824, J. Antibiot., 61, 680, 10.1038/ja.2008.96 · DOI
  135. Kolobova, 2019, Oxidation of a wood extractive betulin to biologically active oxo-derivatives using supported gold catalysts, Green Chem., 21, 3370, 10.1039/C9GC00949C · DOI
  136. Koumaki, 2017, Environmental fate of non-steroidal anti-inflammatory drugs in river water/sediment systems, J. Hazard. Mater., 323, 233, 10.1016/j.jhazmat.2016.03.026 · DOI
  137. Kovalakova, 2020, Occurrence and toxicity of antibiotics in the aquatic environment: a review, Chemosphere, 251, 126351, 10.1016/j.chemosphere.2020.126351 · DOI
  138. Kreit, 2017, Microbial catabolism of sterols: focus on the enzymes that transform the sterol 3β-hydroxy-5-en into 3-keto-4-en, FEMS Microbiol. Lett., 364, fnx007, 10.1093/FEMSLE/FNX007 · DOI
  139. Kuhl, 2021, Genome-based characterization of plant-associated Rhodococcus qingshengii RL1 reveals stress tolerance and plant–microbe interaction traits, Front. Microbiol., 12, 708605, 10.3389/FMICB.2021.708605 · DOI
  140. Kulkarni, 2022, Nanoparticles derived from Rhodococcus kroppenstedtii as bioactive agents for controlling aquaculture associated bacterial pathogens, Aquaculture, 547, 737538, 10.1016/J.AQUACULTURE.2021.737538 · DOI
  141. Kümmerer, 2010, Why green and sustainable pharmacy, 3, 10.1007/978-3-642-05199-9_1 · DOI
  142. Kurosawa, 2008, Rhodostreptomycins, antibiotics biosynthesized following horizontal gene transfer from Streptomyces padanus to Rhodococcus fascians, J. Am. Chem. Soc., 130, 1126, 10.1021/JA077821P · DOI
  143. Kuyukina, Bioremediation of contaminated environments using Rhodococcus, 231
  144. Kuyukina, Production of trehalolipid biosurfactants by Rhodococcus, 271
  145. Kuyukina, 2015, Trehalolipid biosurfactants from nonpathogenic Rhodococcus actinobacteria with diverse immunomodulatory activities, New Biotechnol., 32, 559, 10.1016/J.NBT.2015.03.006 · DOI
  146. Kwasiborski, 2015, Transcriptome of the quorum-sensing signal-degrading Rhodococcus erythropolis responds differentially to virulent and avirulent Pectobacterium atrosepticum, Heredity, 114, 476, 10.1038/hdy.2014.121 · DOI
  147. Ladkau, 2014, The microbial cell – functional unit for energy dependent multistep biocatalysis, Curr. Opin. Biotechnol., 30, 178, 10.1016/J.COPBIO.2014.06.003 · DOI
  148. Larcher, 2011, Biodegradation of sulfamethoxazole by individual and mixed bacteria, Appl. Microbiol. Biotechnol., 91, 211, 10.1007/s00253-011-3257-8 · DOI
  149. Larcher, 2012, The effect of ozone on the biodegradation of 17α-ethinylestradiol and sulfamethoxazole by mixed bacterial cultures, Appl. Microbiol. Biotechnol., 97, 2201, 10.1007/S00253-012-4054-8 · DOI
  150. Larcher, 2013, Biodegradation of 17α-ethinylestradiol by heterotrophic bacteria, Environ. Pollut., 173, 17, 10.1016/j.envpol.2012.10.028 · DOI
  151. Larkin, 2006, Biodegradation by members of the genus Rhodococcus: biochemistry, physiology, and genetic adaptation, Adv. Appl. Microbiol., 59, 1, 10.1016/S0065-2164(06)59001-X · DOI
  152. Larkin, 2010, Genomes and plasmids in Rhodococcus, 73, 10.1007/978-3-642-12937-7_3 · DOI
  153. Leder, 2015, Putting benign by design into practice-novel concepts for green and sustainable pharmacy: designing green drug derivatives by non-targeted synthesis and screening for biodegradability, Sustain. Chem. Pharm., 2, 31, 10.1016/J.SCP.2015.07.001 · DOI
  154. Lee, 2013, Effects of growth substrate on triclosan biodegradation potential of oxygenase-expressing bacteria, Chemosphere, 93, 1904, 10.1016/j.chemosphere.2013.06.069 · DOI
  155. Lee, 2020, Preparation of a mesoporous silica quorum quenching medium for wastewater treatment using a membrane bioreactor, Biofouling, 36, 369, 10.1080/08927014.2020.1749601 · DOI
  156. Li, 2022, An amidase and a novel phenol hydroxylase catalyze the degradation of the antibacterial agent triclocarban by Rhodococcus rhodochrous, J. Hazard. Mater., 430, 128444, 10.1016/J.JHAZMAT.2022.128444 · DOI
  157. Li, 2012, Rhodococcus nanhaiensis sp. nov., an actinobacterium isolated from marine sediment, Int. J. Syst. Evol. Microbiol., 62, 2517, 10.1099/ijs.0.038067-0 · DOI
  158. Li, 2021, An electrochemical membrane biofilm reactor for removing sulfonamides from wastewater and suppressing antibiotic resistance development: performance and mechanisms, J. Hazard. Mater., 404, 124198, 10.1016/j.jhazmat.2020.124198 · DOI
  159. Liang, 2021, Genetic toolkits for engineering Rhodococcus species with versatile applications, Biotechnol. Adv., 49, 107748, 10.1016/j.biotechadv.2021.107748 · DOI
  160. Lin, 2017, Whole-cell biocatalysts by design, Microb. Cell Factories, 16, 106, 10.1186/S12934-017-0724-7 · DOI
  161. Lin, 2022, Post COVID-19 pandemic: disposable face masks as a potential vector of antibiotics in freshwater and seawater, Sci. Total Environ., 820, 153049, 10.1016/J.SCITOTENV.2022.153049 · DOI
  162. Liu, 2016, Isolation, immobilization, and degradation performance of the 17β-estradiol-degrading bacterium Rhodococcus sp. JX-2, Water Air Soil Pollut., 227, 422, 10.1007/s11270-016-3122-6 · DOI
  163. Liu, 2006, Biocatalytic synthesis of highly enantiopure 1,4-benzodioxane-2-carboxylic acid and amide, Chin. J. Chem., 24, 1665, 10.1002/cjoc.200690312 · DOI
  164. Liu, 2017, pH stress-induced cooperation between Rhodococcus ruber YYL and Bacillus cereus MLY1 in biodegradation of tetrahydrofuran, Front. Microbiol., 8, 2297, 10.3389/FMICB.2017.02297 · DOI
  165. Liu, 2019, Metabolite cross-feeding between Rhodococcus ruber YYL and Bacillus cereus MLY1 in the biodegradation of tetrahydrofuran under pH stress, Appl. Environ. Microbiol., 85, e01196, 10.1128/AEM.01196-19 · DOI
  166. Lucien, 2021, Antibiotics and antimicrobial resistance in the COVID-19 era: perspective from resource-limited settings, Int. J. Infect. Dis., 104, 250, 10.1016/j.ijid.2020.12.087 · DOI
  167. Ma, 2020, Isolation, characterisation, and genome sequencing of Rhodococcus equi: a novel strain producing chitin deacetylase, Sci. Rep., 10, 4329, 10.1038/s41598-020-61349-9 · DOI
  168. MacKay, 2019, D-serine: potential therapeutic agent and/or biomarker in schizophrenia and depression?, Front. Psych., 10, 25, 10.3389/FPSYT.2019.00025 · DOI
  169. Maddela, 2020, Discrepant roles of a quorum quenching bacterium (Rhodococcus sp. BH4) in growing dual-species biofilms, Sci. Total Environ., 713, 136402, 10.1016/J.SCITOTENV.2019.136402 · DOI
  170. Madikizela, 2021, Occurrence and ecotoxicological risk assessment of non-steroidal anti-inflammatory drugs in South African aquatic environment: what is known and the missing information?, Chemosphere, 280, 130688, 10.1016/j.chemosphere.2021.130688 · DOI
  171. Maia, 2018, Enantioselective degradation of ofloxacin and levofloxacin by the bacterial strains Labrys portucalensis F11 and Rhodococcus sp. FP1, Ecotoxicol. Environ. Saf., 155, 144, 10.1016/j.ecoenv.2018.02.067 · DOI
  172. Markovic, 2021, Combined toxicity of therapeutic pharmaceuticals to duckweed, Lemna minor, Ecotoxicol. Environ. Saf., 208, 111428, 10.1016/J.ECOENV.2020.111428 · DOI
  173. Martinez Alvarez, 2022, Identification of key bacterial players during successful full-scale soil field bioremediation in Antarctica, Int. Biodeterior. Biodegradation, 168, 105354, 10.1016/J.IBIOD.2021.105354 · DOI
  174. Marvik, 2020, The systemic challenge of the bioeconomy, EMBO Rep., 21, e51478, 10.15252/EMBR.202051478 · DOI
  175. McLain, 2022, Acetaminophen levels found in recycled wastewater alter soil microbial community structure and functional diversity, Microb. Ecol., 1, 10.1007/S00248-022-02022-8 · DOI
  176. Meena, 2021, Existence in cellulose shelters: industrial and pharmaceutical leads of symbiotic actinobacteria from ascidian Phallusia nigra, Andaman Islands, World J. Microbiol. Biotechnol., 37, 120, 10.1007/S11274-021-03090-7 · DOI
  177. Mejía-García, 2020, Overview of non-steroidal anti-inflammatory drugs as emerging contaminants, 41, 10.1007/698_2020_541 · DOI
  178. Menashe, 2020, Biodegradation of the endocrine-disrupting chemical 17α-ethynylestradiol (EE2) by Rhodococcus zopfii and pseudomonas putida encapsulated in small bioreactor platform (SBP) capsules, Appl. Sci., 10, 336, 10.3390/APP10010336 · DOI
  179. Mezzelani, 2016, Ecotoxicological potential of non-steroidal anti-inflammatory drugs (NSAIDs) in marine organisms: bioavailability, biomarkers and natural occurrence in Mytilus galloprovincialis, Mar. Environ. Res., 121, 31, 10.1016/J.MARENVRES.2016.03.005 · DOI
  180. Mezzelani, 2018, Long-term exposure of Mytilus galloprovincialis to diclofenac, ibuprofen and ketoprofen: insights into bioavailability, biomarkers and transcriptomic changes, Chemosphere, 198, 238, 10.1016/j.chemosphere.2018.01.148 · DOI · Paper page
  181. Mishra, 2021, Recent advanced technologies for the characterization of xenobiotic-degrading microorganisms and microbial communities, Front. Bioeng. Biotechnol., 9, 632059, 10.3389/FBIOE.2021.632059 · DOI
  182. Mohd Hanafiah, 2022, The occurrence of non-steroidal anti-inflammatory drugs (NSAIDs) in Malaysian urban domestic wastewater, Chemosphere, 287, 132134, 10.1016/J.CHEMOSPHERE.2021.132134 · DOI
  183. Mohn, 2012, Gene cluster encoding cholate catabolism in Rhodococcus spp, J. Bacteriol., 194, 6712, 10.1128/JB.01169-12 · DOI
  184. Morales-Paredes, 2022, Pharmaceutical compounds used in the COVID-19 pandemic: a review of their presence in water and treatment techniques for their elimination, Sci. Total Environ., 814, 152691, 10.1016/J.SCITOTENV.2021.152691 · DOI
  185. Moreno-Opo, 2021, The veterinary use of diclofenac and vulture conservation in Spain: updated evidence and socio-ecological implications, Sci. Total Environ., 796, 148851, 10.1016/J.SCITOTENV.2021.148851 · DOI
  186. Mulkiewicz, 2021, Metabolism of non-steroidal anti-inflammatory drugs by non-target wild-living organisms, Sci. Total Environ., 791, 148251, 10.1016/J.SCITOTENV.2021.148251 · DOI
  187. Muscaritoli, 2021, The impact of nutrients on mental health and well-being: insights from the literature, Front. Nutr., 8, 656290, 10.3389/FNUT.2021.656290 · DOI
  188. Myosho, 2021, Effects of synthetic sex steroid hormone exposures on gonadal sex differentiation and dynamics of a male-related gene, gonadal soma-derived factor (Gsdf) and an estrogen up-regulated gene, Choriogenine-H (ChgH) gene expression in the euryhaline Javafish medaka, Oryzias javanicus, based on genetic sexes, Chemosphere, 274, 129893, 10.1016/J.CHEMOSPHERE.2021.129893 · DOI
  189. Nachtigall, 2010, Two new aurachins from Rhodococcus sp. Acta 2259, J. Antibiot., 63, 567, 10.1038/ja.2010.79 · DOI
  190. Nagaraja, 2020, A machine learning approach for efficient selection of enzyme concentrations and its application for flux optimization, Catalysts, 10, 291, 10.3390/CATAL10030291 · DOI
  191. Naicker, 2015, Antimicrobial and antioxidant activities of piperidine derivatives, Afr. J. Pharm. Pharmacol., 9, 783, 10.5897/AJPP2015.4335 · DOI
  192. Nain, 2020, Computational prediction of active sites and ligands in different AHL quorum quenching lactonases and acylases, J. Biosci., 45, 26, 10.1007/S12038-020-0005-1 · DOI
  193. Narayan, 2015, Enzymatic hydroxylation of an unactivated methylene C–H bond guided by molecular dynamics simulations, Nat. Chem., 7, 653, 10.1038/nchem.2285 · DOI
  194. Nebot, 2015, Introduction of human pharmaceuticals from wastewater treatment plants into the aquatic environment: a rural perspective, Environ. Sci. Pollut. Res., 22, 10559, 10.1007/s11356-015-4234-z · DOI
  195. Nogovitsina, 2011, Biocatalytic synthesis of pharmacology perspective stigmast-4-en-3-one using rhodococci cells, Russ. J. Bioorg. Chem., 37, 626, 10.1134/S1068162011050128 · DOI
  196. Oaks, 2004, Diclofenac residues as the cause of vulture population decline in Pakistan, Nature, 427, 630, 10.1038/nature02317 · DOI
  197. O’Grady, 2009, Removal of aqueous 17α-ethinylestradiol by Rhodococcus species, Environ. Eng. Sci., 26, 1393, 10.1089/ees.2008.0272 · DOI
  198. Oh, 2013, Biofouling inhibition in MBR by Rhodococcus sp. BH4 isolated from real MBR plant, Appl. Microbiol. Biotechnol., 97, 10223, 10.1007/S00253-013-4933-7 · DOI
  199. Olivera, 2019, Steroids as environmental compounds recalcitrant to degradation: genetic mechanisms of bacterial biodegradation pathways, Genes, 10, 512, 10.3390/GENES10070512 · DOI
  200. Orro, 2015, Genome and phenotype microarray analyses of Rhodococcus sp. BCP1 and Rhodococcus opacus R7: genetic determinants and metabolic abilities with environmental relevance, PLoS One, 10, e0139467, 10.1371/journal.pone.0139467 · DOI

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Cited by

Sorted by citations

Authors
Ivshina, I., Bazhutin, G., & Tyumina, E.
Affiliations
Ural Branch of the Russian Academy of Sciences (RU)
ORCID
Venue
Frontiers in Microbiology
Publisher
Frontiers Media SA
Published
2022-09-29
Volume
13
Pages
967127-967127
ISSN
1664-302X (ISSN-L)
DOI
10.3389/fmicb.2022.967127
PMC
PMC9557007
OpenAlex
W4298003470
Cited by
62
References
2
Citations by year
2026: 14 · 2025: 21 · 2024: 15 · 2023: 11 · 2022: 1
FWCI
0.67
Type
journal article
Language
en
Open access
Licence
Locations
  • Frontiers in Microbiology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Ural Branch, Russian Academy of Sciences
  • Russian Science Foundation (21-14-00132)
Topics
  • Physical Sciences › Environmental Science › Pollution › Pharmaceutical and Antibiotic Environmental Impacts
  • Physical Sciences › Environmental Science › Pollution › Microbial bioremediation and biosurfactants
  • Physical Sciences › Chemistry › Organic Chemistry › Steroid Chemistry and Biochemistry
Keywords
Rhodococcus, pharmaceutical pollutants, biodegradation, biotransformation, natural attenuation, quorum sensing inhibition, biocatalysis, biosurfactants, antimicrobial agents, natural products, nanostructures, environmental pollution, drinking water contamination, bioremediation

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