Six-color intravital two-photon imaging of brain tumors and their dynamic microenvironment
Open accessAbstract
The majority of intravital studies on brain tumor in living animal so far rely on dual color imaging. We describe here a multiphoton imaging protocol to dynamically characterize the interactions between six cellular components in a living mouse. We applied this methodology to a clinically relevant glioblastoma multiforme (GBM) model designed in reporter mice with targeted cell populations labeled by fluorescent proteins of different colors. This model permitted us to make non-invasive longitudinal and multi-scale observations of cell-to-cell interactions. We provide examples of such 5D (x,y,z,t,color) images acquired on a daily basis from volumes of interest, covering most of the mouse parietal cortex at subcellular resolution. Spectral deconvolution allowed us to accurately separate each cell population as well as some components of the extracellular matrix. The technique represents a powerful tool for investigating how tumor progression is influenced by the interactions of tumor cells with host cells and the extracellular matrix micro-environment. It will be especially valuable for evaluating neuro-oncological drug efficacy and target specificity. The imaging protocol provided here can be easily translated to other mouse models of neuropathologies, and should also be of fundamental interest for investigations in other areas of systems biology.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
IVM technique has been developed as an integration of methods including CLSM, multiphoton microscopy, and epifluorescence to dynamically evaluate tumor structure and physiological courses from tissue to subcellular level.
“IVM technique has been developed as an integration of methods including CLSM, multiphoton microscopy, and epifluorescence to dynamically evaluate tumor structure and physiological courses from tissue to subcellular level [149,150].”
From Approaches to Improve EPR-Based Drug Delivery for Cancer Therapy and Diagnosis (2023) · cites this paper as [149]
The Introduction and Results and discussions describe repeated intravital two-photon imaging of brain tumors, from large fields of view to subcellular detail. Experimental setup and image processing says the microscope collected epifluorescence signals after two-photon excitation. The paper does not describe integrating confocal laser scanning microscopy (CLSM), and it treats epifluorescence as a collected signal rather than a separate imaging method. Page numbers were not provided in our copy. (LLM, conf=0.86)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Hallmarks of Cancer: The Next Generation
Hanahan, D., & Weinberg, R. A. (2011) · Cell · doi:10.1016/j.cell.2011.02.013 · Cited by 66,590 · see citing works
Astrocyte-endothelial interactions at the blood-brain barrier
Abbott, N. J., Rönnbäck, L., & Hansson, E. (2006) · Nature Reviews Neuroscience · doi:10.1038/nrn1824 · Cited by 5,595 · see citing works
Deep tissue two-photon microscopy
Helmchen, F., & Denk, W. T. (2005) · Nature Methods · doi:10.1038/nmeth818 · Cited by 4,165 · see citing works
Coordinated regulation of myeloid cells by tumours
Gabrilovich, D. I., Ostrand-Rosenberg, S., & Bronte, V. (2012) · Nature Reviews Immunology · doi:10.1038/nri3175 · Cited by 3,544 · see citing works
Imaging Neuronal Subsets in Transgenic Mice Expressing Multiple Spectral Variants of GFP
Feng, G., Mellor, R. H., Bernstein, M., Keller-Peck, C., Nguyen, Q. T., Wallace, M., Nerbonne, J. M., Lichtman, J. W., & Sanes, J. R. (2000) · Neuron · doi:10.1016/s0896-6273(00)00084-2 · Cited by 3,042 · see citing works
Long-term in vivo imaging of experience-dependent synaptic plasticity in adult cortex
Trachtenberg, J. T., Chen, B. E., Knott, G. W., Feng, G., Sanes, J. R., Welker, E., & Svoboda, K. (2002) · Nature · doi:10.1038/nature01273 · Cited by 1,927 · see citing works
DeAngelis, L. M. (2001) · New England Journal of Medicine · doi:10.1056/nejm200101113440207 · Cited by 1,689 · see citing works
Pochet, P. (2006) · Griffith Research Online · Cited by 1,550 · see citing works
Primary brain tumours in adults
Ricard, D., Idbaïh, A., Ducray, F., Lahutte, M., Hoang‐Xuan, K., & Delattre, J. (2012) · The Lancet · doi:10.1016/s0140-6736(11)61346-9 · Cited by 1,200 · see citing works
Visualizing dendritic cell networks in vivo
Lindquist, R. L., Shakhar, G., Dudziak, D., Wardemann, H., Eisenreich, T., Dustin, M. L., & Nussenzweig, M. C. (2004) · Nature Immunology · doi:10.1038/ni1139 · Cited by 806 · see citing works
The brain tumor microenvironment
Charles, N. A., Holland, E. C., Gilbertson, R., Glass, R., & Kettenmann, H. (2011) · Glia · doi:10.1002/glia.21136 · Cited by 718 · see citing works
The brain tumor microenvironment
Charles, N. A., Holland, E. C., Gilbertson, R. J., Glaß, R., & Kettenmann, H. (2011) · Glia · doi:10.1002/glia.21264 · Cited by 712 · see citing works
Deep tissue multiphoton microscopy using longer wavelength excitation
Kobat, D., Durst, M. E., Nishimura, N., Wong, A. W., Schaffer, C. B., & Xu, C. (2009) · Optics Express · doi:10.1364/oe.17.013354 · Cited by 577 · see citing works
Prins, R. M., Soto, H., Konkankit, V., Odesa, S. K., Eskin, A., Yong, W. H., Nelson, S. F., & Liau, L. M. (2010) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-10-2563 · Cited by 448 · see citing works
Zimmermann, T., Rietdorf, J., Girod, A., Georget, V., & Pepperkok, R. (2002) · FEBS Letters · doi:10.1016/s0014-5793(02)03508-1 · Cited by 242 · see citing works
Piao, Y., Liang, J., Holmes, L., Zurita, A. J., Henry, V., Heymach, J. V., & de Groot, J. (2012) · Neuro-Oncology · doi:10.1093/neuonc/nos158 · Cited by 227 · see citing works
Winkler, F., Kienast, Y., Fuhrmann, M., von Baumgarten, L., Burgold, S., Mitteregger, G., Kretzschmar, H. A., & Herms, J. W. (2009) · Glia · doi:10.1002/glia.20850 · Cited by 210 · see citing works
Two-Photon and Second Harmonic Microscopy in Clinical and Translational Cancer Research
Perry, S. W., Burke, R. M., & Brown, E. B. (2012) · Annals of Biomedical Engineering · doi:10.1007/s10439-012-0512-9 · Cited by 209 · see citing works
Microglia/macrophages promote glioma progression
Zhai, H., Heppner, F. L., & Tsirka, S. E. (2010) · Glia · doi:10.1002/glia.21117 · Cited by 209 · see citing works
In vivo imaging of the diseased nervous system
Misgeld, T., & Kerschensteiner, M. (2006) · Nature reviews. Neuroscience · doi:10.1038/nrn1905 · Cited by 197 · see citing works
From molecular structure to tissue architecture: collagen organization probed by SHG microscopy
Cicchi, R., Vogler, N., Kapsokalyvas, D., Dietzek, B., Popp, J., & Pavone, F. S. (2012) · Journal of Biophotonics · doi:10.1002/jbio.201200092 · Cited by 191 · see citing works
Dray, C., Rougon, G., & Debarbieux, F. C. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0900222106 · Cited by 179 · see citing works
A simple introduction to multiphoton microscopy
Ustione, A., & Piston, D. W. (2011) · Journal of Microscopy · doi:10.1111/j.1365-2818.2011.03532.x · Cited by 172 · see citing works
Bevacizumab Has Differential and Dose-Dependent Effects on Glioma Blood Vessels and Tumor Cells
von Baumgarten, L., Brucker, D., Tirniceru, A., Kienast, Y., Grau, S. J., Burgold, S., Herms, J. W., & Winkler, F. (2011) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-10-1868 · Cited by 164 · see citing works
Optimizing imaging parameters for the separation of multiple labels in a fluorescence image
Neher, R. E., & Neher, E. (2003) · Journal of Microscopy · doi:10.1111/j.1365-2818.2004.01262.x · Cited by 133 · see citing works
Prados, M. D., Seiferheld, W. F., Sandler, H. M., Buckner, J. C., Phillips, T. L., Schultz, C. J., Urtasun, R. C., Davis, R. L., Gutin, P. H., Cascino, T. L., et al. (2004) · International Journal of Radiation Oncology*Biology*Physics · doi:10.1016/j.ijrobp.2003.08.024 · Cited by 128 · see citing works
Fenrich, K. K., Weber, P., Hocine, M., Zalc, M., Rougon, G. N., & Debarbieux, F. C. (2012) · The Journal of Physiology · doi:10.1113/jphysiol.2012.230532 · Cited by 115 · see citing works
The Murine GL261 Glioma Experimental Model to Assess Novel Brain Tumor Treatments
Newcomb, E., & Zagzag, D. (2009) · Humana Press eBooks · doi:10.1007/978-1-60327-553-8_12 · Cited by 73 · see citing works
Intravital Imaging of Neutrophil Recruitment in Hepatic Ischemia-Reperfusion Injury in Mice
Honda, M., Takeichi, T., Asonuma, K., Tanaka, K., Kusunoki, M., & Inomata, Y. (2013) · Transplantation · doi:10.1097/tp.0b013e31827d62b5 · Cited by 55 · see citing works
Dual reporter approaches for identification of Cre efficacy and astrocyte heterogeneity
Degen, J., Dublin, P., Zhang, J., Dobrowolski, R., Jokwitz, M., Karram, K., Trotter, J., Jabs, R., Willecke, K., Steinhäuser, C., et al. (2012) · The FASEB Journal · doi:10.1096/fj.12-207183 · Cited by 31 · see citing works
Ricard, C., Stanchi, F., Rodriguez, T., Amoureux, M., Rougon, G. N., & Debarbieux, F. C. (2013) · PLoS ONE · doi:10.1371/journal.pone.0072655 · Cited by 28 · see citing works
Vérant, P., Serduc, R., van der Sanden, B. P. J., Chantal, R., Ricard, C., Coles, J. A., & Vial, J. (2008) · Journal of Biomedical Optics · doi:10.1117/1.2870083 · Cited by 19 · see citing works
Kuijpers, M. J. E., & Heemskerk, J. W. M. (2011) · Methods in molecular biology · doi:10.1007/978-1-61779-307-3_1 · Cited by 11 · see citing works
Zhuo, S., Wu, G., Chen, J. X., Zhu, X., & Xie, S. (2012) · Laser Physics Letters · doi:10.7452/lapl.201210006 · Cited by 10 · see citing works
Ricard, C., Vacca, B., & Weber, P. (2012) · Journal of Anatomy · doi:10.1111/j.1469-7580.2012.01529.x · Cited by 9 · see citing works
Short-term effects of synchrotron irradiation on vasculature and tissue in healthy mouse brain
Ricard, C., Fernández, M., Gastaldo, J., Dupin, L., Somveille, L., Farion, R., Requardt, H., Vial, J., Elleaume, H., Segebarth, C. M., et al. (2009) · Journal of Synchrotron Radiation · doi:10.1107/s0909049509015428 · Cited by 9 · see citing works
Dynamics of Rapid Spore Capture by Dendritic Cells in the Lung Alveolus
Fiole, D., Douady, J., Vial, J., Quesnel‐Hellmann, A., & Tournier, J. (2012) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.201110-1782im · Cited by 8 · see citing works
Two-Photon Imaging of Immune Cells in Neural TissuePreprint
Niesner, R. A., Siffrin, V., & Zipp, F. (2013) · Cold Spring Harbor Protocols · doi:10.1101/pdb.prot073528 · Cited by 6 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Microenvironmental regulation of therapeutic response in cancer
Klemm, F., & Joyce, J. A. (2014) · Trends in Cell Biology · doi:10.1016/j.tcb.2014.11.006 · Cited by 769 · see citing works
Intravital imaging by simultaneous label-free autofluorescence-multiharmonic microscopy
You, S., Tu, H., Chaney, E. J., Sun, Y., Zhao, Y., Bower, A. J., Liu, Y.-Z., Marjanovic, M., Sinha, S., Pu, Y., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-04470-8 · Cited by 271 · see citing works
MemBright: A Family of Fluorescent Membrane Probes for Advanced Cellular Imaging and Neuroscience
Collot, M., Ashokkumar, P., Anton, H., Boutant, E., Faklaris, O., Galli, T., Mély, Y., Danglot, L., & Klymchenko, A. S. (2019) · Cell chemical biology · doi:10.1016/j.chembiol.2019.01.009 · Cited by 230 · see citing works
Watts, E., Zhao, Y., Dhara, A., Eller, B., Patwardhan, A. R., & Sinai, A. P. (2015) · mBio · doi:10.1128/mbio.01155-15 · Cited by 224 · see citing works
An Experimenter’s Guide to Glioblastoma Invasion Pathways
de Gooijer, M. C., Navarro, M. G., Bernards, R., Würdinger, T., & van Tellingen, O. (2018) · Trends in Molecular Medicine · doi:10.1016/j.molmed.2018.07.003 · Cited by 123 · see citing works
Murty, S., Haile, S. T., Beinat, C., Aalipour, A., Alam, I. S., Murty, T., Shaffer, T. M., Patel, C. B., Graves, E. E., Mackall, C. L., et al. (2020) · OncoImmunology · doi:10.1080/2162402x.2020.1757360 · Cited by 98 · see citing works
Approaches to Improve EPR-Based Drug Delivery for Cancer Therapy and Diagnosis
Subhan, M. A., Parveen, F., Filipczak, N., Yalamarty, S. S. K., & Torchilin, V. P. (2023) · Journal of Personalized Medicine · doi:10.3390/jpm13030389 · Cited by 98 · see citing works
Chen, Z., Ross, J., & Hambardzumyan, D. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1902366116 · Cited by 93 · see citing works
In vivo NIR-II structured-illumination light-sheet microscopy
Wang, F., Ma, Z., Zhong, Y., Salazar, F., Xu, C., Ren, F., Qu, L., Wu, A. M., & Dai, H. (2021) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2023888118 · Cited by 85 · see citing works
Robust blind spectral unmixing for fluorescence microscopy using unsupervised learning
McRae, T. D., Oleksyn, D. W., Miller, J., & Gao, Y. (2019) · PLoS ONE · doi:10.1371/journal.pone.0225410 · Cited by 78 · see citing works
Rakhymzhan, A. A., Leben, R., Zimmermann, H. G., Günther, R., Mex, P., Reismann, D., Ulbricht, C., Acs, A., Brandt, A. U., Lindquist, R. L., et al. (2017) · Scientific Reports · doi:10.1038/s41598-017-07165-0 · Cited by 58 · see citing works
Faisal, S. M., Comba, A., Varela, M. L., Argento, A. E., Brumley, E. K., Abel, C. C., Castro, M. G., & Lowenstein, P. R. (2022) · Frontiers in Oncology · doi:10.3389/fonc.2022.1005069 · Cited by 57 · see citing works
Phenotypic dynamics of microglial and monocyte-derived cells in glioblastoma-bearing mice
Ricard, C., Tchoghandjian, A., Luche, H., Grenot, P., Figarella-Branger, D. F., Rougon, G. N., Malissen, M., & Debarbieux, F. C. (2016) · Scientific Reports · doi:10.1038/srep26381 · Cited by 57 · see citing works
Two-photon probes for in vivo multicolor microscopy of the structure and signals of brain cells
Ricard, C., Arroyo, E. D., He, C. X., Portera‐Cailliau, C., Lepousez, G., Canepari, M., & Fiole, D. (2018) · Brain Structure and Function · doi:10.1007/s00429-018-1678-1 · Cited by 54 · see citing works
Intravital microscopy of dynamic single-cell behavior in mouse mammary tissue
Dawson, C. A., Mueller, S. N., Lindeman, G. J., Rios, A. C., & Visvader, J. E. (2021) · Nature Protocols · doi:10.1038/s41596-020-00473-2 · Cited by 49 · see citing works
Urban, A., Golgher, L., Brunner, C., Gdalyahu, A., Har-Gil, H., Kain, D., Montaldo, G., Sironi, L., & Blinder, P. (2017) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2017.07.018 · Cited by 44 · see citing works
Frontiers in intravital multiphoton microscopy of cancer
Perrin, L., Bayarmagnai, B., & Gligorijevic, B. (2019) · Cancer Reports · doi:10.1002/cnr2.1192 · Cited by 42 · see citing works
Bares, A. J., Mejooli, M. A., Pender, M. A., Leddon, S. A., Tilley, S., Lin, K., Dong, J., Kim, M., Fowell, D. J., Nishimura, N., et al. (2020) · Optica · doi:10.1364/optica.389982 · Cited by 33 · see citing works
Label-Free Deep Profiling of the Tumor Microenvironment
You, S., Chaney, E. J., Tu, H., Sun, Y., Sinha, S., & Boppart, S. A. (2021) · Cancer Research · doi:10.1158/0008-5472.can-20-3124 · Cited by 33 · see citing works
Zomer, A., Croci, D., Kowal, J., van Gurp, L., & Joyce, J. A. (2022) · iScience · doi:10.1016/j.isci.2022.104570 · Cited by 33 · see citing works
Simon, A., Yang, M., Marrison, J., James, A. D., Hunt, M. J., O’Toole, P. J., Kaye, P. M., Whittington, M. A., Chawla, S., & Brackenbury, W. J. (2020) · Journal of Neuroinflammation · doi:10.1186/s12974-020-01753-0 · Cited by 26 · see citing works
Current trends in the characterization and monitoring of vascular response to cancer therapy
Shrestha, B., Stern, N. B., Zhou, A., Dunn, A. K., & Porter, T. M. (2024) · Cancer Imaging · doi:10.1186/s40644-024-00767-8 · Cited by 25 · see citing works
3D imaging for driving cancer discovery
van Ineveld, R. L., van Vliet, E. J., Wehrens, E. J., Alieva, M., & Rios, A. C. (2022) · The EMBO Journal · doi:10.15252/embj.2021109675 · Cited by 26 · see citing works
Live-cell fluorescence spectral imaging as a data science challenge
Acuña-Rodriguez, J. P., Mena-Vega, J. P., & Argüello‐Miranda, O. (2022) · Biophysical Reviews · doi:10.1007/s12551-022-00941-x · Cited by 23 · see citing works
Villette, V., Levesque, M., Miled, A., Gosselin, B. J., & Topolnik, L. (2017) · Scientific Reports · doi:10.1038/srep43388 · Cited by 21 · see citing works
Shukla, S., Dorand, R. D., Myers, J., Woods, S. E., Gulati, N. M., Stewart, P. L., Commandeur, U., Huang, A. Y.-C., & Steinmetz, N. F. (2016) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.6b00060 · Cited by 21 · see citing works
Jiang, L., Wang, X., Wu, Z., Du, H., Wang, S., Li, L., Fang, N., Lin, P., Chen, J., Kang, D., et al. (2017) · Laser Physics Letters · doi:10.1088/1612-202x/aa7c9a · Cited by 21 · see citing works
Greathouse, K. M., Palladino, S. P., Dong, C., Helton, E. S., & Ubogu, E. E. (2016) · Journal of Neuroinflammation · doi:10.1186/s12974-015-0469-3 · Cited by 20 · see citing works
Owyong, M., Hosseini-Nassab, N., Efe, G., Honkala, A. T., van den Bijgaart, R. J. E., Plaks, V., & Smith, B. R. (2017) · Drug Resistance Updates · doi:10.1016/j.drup.2017.10.001 · Cited by 19 · see citing works
Intravital microscopy: Imaging host–parasite interactions in the brain
Niz, M. D., Nacer, A., & Frischknecht, F. (2019) · Cellular Microbiology · doi:10.1111/cmi.13024 · Cited by 18 · see citing works
Sadashivaiah, V., Tippani, M., Page, S. C., Kwon, S., Bach, S. V., Bharadwaj, R. A., Hyde, T. M., Kleinman, J. E., Jaffe, A. E., & Maynard, K. R. (2023) · BMC Neuroscience · doi:10.1186/s12868-022-00765-1 · Cited by 16 · see citing works
Brain tumor delineation enhanced by moxifloxacin-based two-photon/CARS combined microscopy
Le, V., Yoo, S. W., Yoon, Y., Wang, T., Kim, B., Lee, S., Lee, K., Kim, K. H., & Chung, E. (2017) · Biomedical Optics Express · doi:10.1364/boe.8.002148 · Cited by 16 · see citing works
Next-gen tools in cancer neuroscience
Thiel, V., Sur, D., Picoli, C. C., McErlain, T., Couto, K., Simon, D. J., Pan, Y., Dixon, K. O., Kulkarni, R. P., Talbot, S., et al. (2025) · Cell Reports · doi:10.1016/j.celrep.2025.116258 · Cited by 15 · see citing works
Electrochemical fluorescence modulation enables simultaneous multicolour imaging
Yang, Y., Ma, Y., Macmillan, A., Tilley, R. D., & Gooding, J. J. (2025) · Nature Photonics · doi:10.1038/s41566-025-01672-7 · Cited by 12 · see citing works
Zheng, T., Liversage, A. R., Tehrani, K. F., Call, J. A., Kner, P., & Mortensen, L. J. (2023) · Frontiers in Neuroimaging · doi:10.3389/fnimg.2023.959601 · Cited by 12 · see citing works
Dai, X., Chen, H., Chen, Y., Wu, J., Wang, H., Shi, J. H., Fei, X., Wang, Z., Wang, A., Dong, J., et al. (2015) · Oncology Reports · doi:10.3892/or.2015.4281 · Cited by 12 · see citing works
Two-Photon Excitation Spectra of Various Fluorescent Proteins within a Broad Excitation Range
Leben, R., Lindquist, R. L., Hauser, A. E., Niesner, R. A., & Rakhymzhan, A. A. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms232113407 · Cited by 12 · see citing works
PrismPlus: a mouse line expressing distinct fluorophores in four different brain cell types
Gaire, J., Lee, H. C., Ward, R., Currlin, S., Woolley, A. J., Coleman, J. E., Williams, J. C., & Otto, K. J. (2018) · Scientific Reports · doi:10.1038/s41598-018-25208-y · Cited by 11 · see citing works
3D Whole-Brain Imaging Approaches to Study Brain Tumors
Taranda, J., & Turcan, Ş. (2021) · Cancers · doi:10.3390/cancers13081897 · Cited by 11 · see citing works
Grochmal, J. K., Teo, W., Gambhir, H., Kumar, R., Stratton, J. A., Dhaliwal, R., Brideau, C., Biernaskie, J. A., Stys, P. K., & Midha, R. (2018) · Journal of neurosurgery · doi:10.3171/2017.6.jns17239 · Cited by 10 · see citing works
Rakhymzhan, A. A., Acs, A., Hauser, A. E., Winkler, T., & Niesner, R. A. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22116046 · Cited by 9 · see citing works
Hsiao, Y.-T., Huang, Y., Borah, B. J., Chen, S., & Sun, C. (2021) · Biomedical Optics Express · doi:10.1364/boe.428771 · Cited by 9 · see citing works
Multiparametric Imaging of Organ System Interfaces
Vandoorne, K., & Nahrendorf, M. (2017) · Circulation Cardiovascular Imaging · doi:10.1161/circimaging.116.005613 · Cited by 8 · see citing works
Glioblastoma Multiforme heterogeneity profiling with solid-state micropores
Abdallah, M. G., Almugaiteeb, T., Raza, M. U., Battiste, J. D., Kim, Y., & Iqbal, S. M. (2019) · Biomedical Microdevices · doi:10.1007/s10544-019-0416-7 · Cited by 8 · see citing works
Two-photon fluorescence imaging using a tunable spectral window based on fiber supercontinuum
Chen, Z., Huang, J., Huang, X., Gao, X., Zhou, Y., & Fu, L. (2023) · Optics Letters · doi:10.1364/ol.485028 · Cited by 8 · see citing works
Ricard, C., Lamasse, L., Jaouën, A., Rougon, G. N., & Debarbieux, F. C. (2016) · Biomedical Optics Express · doi:10.1364/boe.7.002362 · Cited by 8 · see citing works
Synchronously pumped Raman laser for simultaneous degenerate and nondegenerate two-photon microscopy
Buttolph, M. L., Mejooli, M. A., Sidorenko, P., Eom, C., Schaffer, C. B., & Wise, F. W. (2021) · Biomedical Optics Express · doi:10.1364/boe.421647 · Cited by 7 · see citing works
Visualization of micro-agents and surroundings by real-time multicolor fluorescence microscopy
Kaya, M., Stein, F., Padmanaban, P., Zhang, Z., Rouwkema, J., Khalil, I. S. M., & Misra, S. (2022) · Scientific Reports · doi:10.1038/s41598-022-17297-7 · Cited by 7 · see citing works
Intravital Imaging of Hepatic Blood Biliary Barrier in Live Mice
Vats, R., Kaminski, T. W., & Pradhan‐Sundd, T. (2021) · Current Protocols · doi:10.1002/cpz1.256 · Cited by 6 · see citing works
Diving head-first into brain intravital microscopy
Suthya, A. R., Wong, C. H. Y., & Bourne, J. H. (2024) · Frontiers in Immunology · doi:10.3389/fimmu.2024.1372996 · Cited by 5 · see citing works
Emerging Applications for Optically Enabled Intravital Microscopic Imaging in Radiobiology
Maeda, A., Kulbatski, I., & DaCosta, R. S. (2015) · Molecular Imaging · doi:10.2310/7290.2015.00022 · Cited by 5 · see citing works
Advances in Intravital Non-Linear Optical Imaging of the Central Nervous System in Rodents
Rougon, G. N., Brasselet, S., & Debarbieux, F. C. (2016) · Brain Plasticity · doi:10.3233/bpl-160028 · Cited by 5 · see citing works
Method for Multiplexed Dynamic Intravital Multiphoton Imaging
Rakhymzhan, A. A., Acs, A., Leben, R., Winkler, T., Hauser, A. E., & Niesner, R. A. (2021) · Methods in molecular biology · doi:10.1007/978-1-0716-1593-5_10 · Cited by 5 · see citing works
TRIO Platform: A Novel Low Profile In vivo Imaging Support and Restraint System for Mice
Voziyanov, V., Kemp, B. S., Dressel, C. A., Ponder, K., & Murray, T. A. (2016) · Frontiers in Neuroscience · doi:10.3389/fnins.2016.00169 · Cited by 3 · see citing works
In Silico Approach to Find an Optimal Strategy in Selective Targeting of Cancer Cells
Raychaudhuri, S. (2016) · Journal of Computer Science & Systems Biology · doi:10.4172/jcsb.1000228 · Cited by 2 · see citing works
Overview of Innovative Mouse Models for Imaging Neuroinflammation
Caravagna, C., Jaouën, A., Debarbieux, F. C., & Rougon, G. N. (2016) · Current Protocols in Mouse Biology · doi:10.1002/cpmo.5 · Cited by 2 · see citing works
Rapid neonatal AAV delivery for adult cortical two-photon imaging of genetically encoded sensors
Wisser, S., Blackwood, E. B., Bera, K., Wasilczuk, A. Z., Looger, L. L., Proekt, A., & Cichon, J. (2025) · iScience · doi:10.1016/j.isci.2025.113898 · Cited by 1 · see citing works
Four-Modal Nonlinear Bioimaging Enabled by a Single Robust Broadband Ultrafast All-Fiber Source
Wen, J., Yang, F., Cao, W. P., Wang, Z., Wang, W., Wei, X., & Yang, Z. (2025) · IEEE Journal of Selected Topics in Quantum Electronics · doi:10.1109/jstqe.2025.3636568 · Cited by 1 · see citing works
Development and Utility of a Spectral-based Approach to Peripheral Nerve Myelin Analysis
Grochmal, J. K. (2015) · Libraries and Cultural Resources (University of Calgary) · doi:10.11575/prism/25647
Hyperspectral Imaging in Live Mouse Cortex Using a 48-Channel Multiphoton Microscope
Bares, A. J., Pender, M. A., Mejooli, M. A., Tilley, S., Chen, K. E., Dong, J., Doerschuk, P. C., & Schaffer, C. B. (2016) · Frontiers in Optics · doi:10.1364/fio.2016.fth5d.5
Dissemination of Diffuse Low-Grade Gliomas: Tools and Molecular Insights
Leventoux, N., Hassani, Z., & Hugnot, J. (2017) · Springer eBooks · doi:10.1007/978-3-319-55466-2_7
New dimensions in intravital multi-photon imaging of immune reactions
Niesner, R. A. (2017) · Journal of Immunological Sciences · doi:10.29245/2578-3009/2018/1.1105
A fast algorithm based on a sylvester-like equation for LS regression with GMRF prior
Wei, Q., Chouzenoux, É., Tourneret, J., & Pesquet, J.-C. (2017) · IEEE International Workshop on Computational Advances in Multi-Sensor Adaptive Processing · doi:10.1109/camsap.2017.8313057
Sadashivaiah, V., Tippani, M., Page, S. C., Kwon, S. H., Bach, S. V., Bharadwaj, R. A., Hyde, T. M., Kleinman, J. E., Jaffe, A. E., & Maynard, K. R. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.01.28.428639
Seeber, F., & Blume, M. (2018) · Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature · doi:10.3410/f.725777117.793551805
Fluorescent anionic cyanine plasma membrane probes for live cell and in vivo imagingPreprint
Danylchuk, D. I., Khalin, I., Suseela, Y. V., Filser, S., Plesnila, N., & Klymchenko, A. S. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.10.17.618786
CoSiDeX: A hyperspectral fluorescent protein resource for highly multiplexed imagingPreprint
Patino, A. V., Chapman, H., Ginzel, J. D., Sills, J. E., Lyerly, H. K., Rogers, B. W., Drut, J. E., & Snyder, J. C. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.05.09.652935
Simon, A., Yang, M., Marrison, J., James, A. D., Hunt, M. J., O’Toole, P. J., Kaye, P. M., Whittington, M. A., Chawla, S., & Brackenbury, W. J. (2020) · Figshare · doi:10.6084/m9.figshare.12010647
Simon, A., Yang, M., Marrison, J., James, A. D., Hunt, M. J., O’Toole, P. J., Kaye, P. M., Whittington, M. A., Chawla, S., & Brackenbury, W. J. (2020) · Figshare · doi:10.6084/m9.figshare.12010632
Simon, A., Yang, M., Marrison, J., James, A. D., Hunt, M. J., O’Toole, P. J., Kaye, P. M., Whittington, M. A., Chawla, S., & Brackenbury, W. J. (2020) · Figshare · doi:10.6084/m9.figshare.12010617
Simon, A., Yang, M., Marrison, J., James, A. D., Hunt, M. J., O’Toole, P. J., Kaye, P. M., Whittington, M. A., Chawla, S., & Brackenbury, W. J. (2020) · Figshare · doi:10.6084/m9.figshare.12010641
Simon, A., Yang, M., Marrison, J., James, A. D., Hunt, M. J., O’Toole, P. J., Kaye, P. M., Whittington, M. A., Chawla, S., & Brackenbury, W. J. (2020) · Figshare · doi:10.6084/m9.figshare.12010623
Simon, A., Yang, M., Marrison, J., James, A. D., Hunt, M. J., O’Toole, P. J., Kaye, P. M., Whittington, M. A., Chawla, S., & Brackenbury, W. J. (2020) · Figshare · doi:10.6084/m9.figshare.12010656
Simon, A., Yang, M., Marrison, J., James, A. D., Hunt, M. J., O’Toole, P. J., Kaye, P. M., Whittington, M. A., Chawla, S., & Brackenbury, W. J. (2020) · Figshare · doi:10.6084/m9.figshare.12010662
- Authors
- Ricard, C., & Debarbieux, F. C.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Debarbieux, Franck Christian
- Venue
- Frontiers in Cellular Neuroscience
- Publisher
- Frontiers Media SA
- Published
- 2014
- Volume
- 8
- Pages
- 57-57
- ISSN
- 1662-5102 (ISSN-L)
- PMC
- PMC3932518
- OpenAlex
- W2169145403
- Cited by
- 75
- References
- 38
- Citations by year
- 2025: 5 · 2024: 3 · 2023: 4 · 2022: 7 · 2021: 10 · 2020: 10 · 2019: 6 · 2018: 6 · 2017: 10 · 2016: 9 · 2015: 4 · 2014: 1
- FWCI
- 4.76
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Frontiers in Cellular Neuroscience (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Agence Nationale de la Recherche
- Institut National de la Santé et de la Recherche Médicale (INCa-DGOS-Inserm 6038)
- Institut National Du Cancer (INCa-DGOS-INSERM-6038)
- Direction Générale de l’offre de Soins
- Canceropôle Provence-Alpes-Côte d’Azur
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Health Sciences › Medicine › Genetics › Glioma Diagnosis and Treatment
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Optical Imaging and Spectroscopy Techniques
- Keywords
- glioblastoma, intravital imaging, two-photon microscopy, tumor microenvironment, cell-cell interactions, fluorescent protein tagging, spectral deconvolution, extracellular matrix, longitudinal imaging, parietal cortex
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
