Mapping of HKT1;5 Gene in Barley Using GWAS Approach and Its Implication in Salt Tolerance Mechanism
Open accessAbstract
Sodium (Na+) accumulation in the cytosol will result in ion homeostasis imbalance and toxicity of transpiring leaves. Studies of salinity tolerance in the diploid wheat ancestor Triticum monococcum showed that HKT1;5-like gene was a major gene in the QTL for salt tolerance, named Nax2. In the present study, we were interested in investigating the molecular mechanisms underpinning the role of the HKT1;5 gene in salt tolerance in barley (Hordeum vulgare). A USDA mini-core collection of 2671 barley lines, part of a field trial was screened for salinity tolerance, and a Genome Wide Association Study (GWAS) was performed. Our results showed important SNPs that are correlated with salt tolerance that mapped to a region where HKT1;5 ion transporter located on chromosome 4. Furthermore, sodium (Na+) and potassium (K+) content analysis revealed that tolerant lines accumulate more sodium in roots and leaf sheaths, than in the sensitive ones. In contrast, sodium concentration was reduced in leaf blades of the tolerant lines under salt stress. In the absence of NaCl, the concentration of Na+ and K+ were the same in the roots, leaf sheaths and leaf blades between the tolerant and the sensitive lines. In order to study the molecular mechanism behind that, alleles of the HKT1;5 gene from 5 tolerant and 5 sensitive barley lines were cloned and sequenced. Sequence analysis did not show the presence of any polymorphism that distinguishes between the tolerant and sensitive alleles. Our real-time RT-PCR experiments, showed that the expression of HKT1;5 gene in roots of the tolerant line was significantly induced after challenging the plants with salt stress. In contrast, in leaf sheaths the expression was decreased after salt treatment. In sensitive lines, there was no difference in the expression of HKT1;5 gene in leaf sheath under control and saline conditions, while a slight increase in the expression was observed in roots after salt treatment. These results provide stronger evidence that HKT1;5 gene in barley play a key role in withdrawing Na+ from the xylem and therefore reducing its transport to leaves. Given all that, these data support the hypothesis that HKT1;5 gene is responsible for
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In barley, functional genes under various stresses have been identified over the years.
“In barley, functional genes under various stresses have been identified over the years”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Hazzouri et al., 2018
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Cites
Controlling the False Discovery Rate: A Practical and Powerful Approach to Multiple Testing
Benjamini, Y., & Hochberg, Y. (1995) · Journal of the Royal Statistical Society Series B: Statistical Methodology · doi:10.1111/j.2517-6161.1995.tb02031.x · Cited by 109,482 · see citing works
Inference of Population Structure Using Multilocus Genotype Data
Pritchard, J. K., Stephens, M., & Donnelly, P. (2000) · Genetics · doi:10.1093/genetics/155.2.945 · Cited by 33,871 · see citing works
Detecting the number of clusters of individuals using the software structure: a simulation study
Evanno, G., Regnaut, S., & Goudet, J. (2005) · Molecular Ecology · doi:10.1111/j.1365-294x.2005.02553.x · Cited by 21,540 · see citing works
Haploview: analysis and visualization of LD and haplotype maps
Barrett, J., Fry, B., Maller, J., & Daly, M. (2005) · Bioinform. · doi:10.1093/bioinformatics/bth457 · Cited by 15,086 · see citing works
Mechanisms of Salinity Tolerance
Munns, R., & Tester, M. (2008) · Annual Review of Plant Biology · doi:10.1146/annurev.arplant.59.032607.092911 · Cited by 13,792 · see citing works
APE: Analyses of Phylogenetics and Evolution in R language
Paradis, E., Claude, J., & Strimmer, K. (2004) · Bioinformatics · doi:10.1093/bioinformatics/btg412 · Cited by 12,037 · see citing works
Principal components analysis corrects for stratification in genome-wide association studies
Price, A. L., Patterson, N. J., Plenge, R. M., Weinblatt, M. E., Shadick, N. A., & Reich, D. (2006) · Nature Genetics · doi:10.1038/ng1847 · Cited by 10,248 · see citing works
Jakobsson, M., & Rosenberg, N. A. (2007) · Bioinformatics · doi:10.1093/bioinformatics/btm233 · Cited by 6,309 · see citing works
Multiple Comparisons Using Rank Sums
Dunn, O. J. (1964) · Technometrics · doi:10.1080/00401706.1964.10490181 · Cited by 5,168 · see citing works
distruct : a program for the graphical display of population structure
Rosenberg, N. A. (2003) · Molecular Ecology Notes · doi:10.1046/j.1471-8286.2003.00566.x · Cited by 4,959 · see citing works
Hardy, O. J., & Vekemans, X. (2002) · Molecular Ecology Notes · doi:10.1046/j.1471-8286.2002.00305.x · Cited by 3,562 · see citing works
Genes and salt tolerance: bringing them together
Munns, R. (2005) · New Phytologist · doi:10.1111/j.1469-8137.2005.01487.x · Cited by 3,049 · see citing works
GAPIT: genome association and prediction integrated tool
Lipka, A. E., Tian, F., Wang, Q., Peiffer, J. A., Li, M., Bradbury, P. J., Gore, M. A., Buckler, E. S., & Zhang, Z. (2012) · Bioinformatics · doi:10.1093/bioinformatics/bts444 · Cited by 2,239 · see citing works
Mixed linear model approach adapted for genome-wide association studies
Zhang, Z., Ersoz, E. S., Lai, C., Todhunter, R. J., Tiwari, H. K., Gore, M. A., Bradbury, P. J., Yu, J., Arnett, D. K., Ordovás, J. M., et al. (2010) · Nature Genetics · doi:10.1038/ng.546 · Cited by 2,132 · see citing works
Flowers, T. J. (2004) · Journal of Experimental Botany · doi:10.1093/jxb/erh003 · Cited by 2,132 · see citing works
Regulation of ion homeostasis under salt stress
Zhu, J. (2003) · Current Opinion in Plant Biology · doi:10.1016/s1369-5266(03)00085-2 · Cited by 2,064 · see citing works
Approaches to increasing the salt tolerance of wheat and other cereals
Munns, R., James, R. A., & Läuchli, A. (2006) · Journal of Experimental Botany · doi:10.1093/jxb/erj100 · Cited by 2,029 · see citing works
A rice quantitative trait locus for salt tolerance encodes a sodium transporter
Ren, Z., Gao, J., Li, L., Cai, X., Huang, W., Chao, D., Zhu, M., Wang, Z., Luan, S., & Lin, H. (2005) · Nature Genetics · doi:10.1038/ng1643 · Cited by 1,503 · see citing works
Sodium transport and salt tolerance in plants
Blumwald, E. (2000) · Current Opinion in Cell Biology · doi:10.1016/s0955-0674(00)00112-5 · Cited by 972 · see citing works
Population genomic and genome-wide association studies of agroclimatic traits in sorghum
Morris, G. P., Ramu, P., Deshpande, S., Hash, C. T., Shah, T. M., Upadhyaya, H. D., Riera‐Lizarazu, O., Brown, P. J., Acharya, C. B., Mitchell, S. E., et al. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1215985110 · Cited by 855 · see citing works
Wheat grain yield on saline soils is improved by an ancestral Na+ transporter gene
Munns, R., James, R. A., Xu, B., Athman, A., Conn, S. J., Jordans, C., Byrt, C. S., Hare, R. A., Tyerman, S. D., Tester, M. A., et al. (2012) · Nature Biotechnology · doi:10.1038/nbt.2120 · Cited by 854 · see citing works
Screening methods for salinity tolerance: a case study with tetraploid wheat
Munns, R., & James, R. A. (2003) · Plant and Soil · doi:10.1023/a:1024553303144 · Cited by 831 · see citing works
Estimators for pairwise relatedness and individual inbreeding coefficients
Ritland, K. (1996) · Genetics Research · doi:10.1017/s0016672300033620 · Cited by 785 · see citing works
Development and implementation of high-throughput SNP genotyping in barley
Close, T. J., Bhat, P. R., Lonardi, S., Wu, Y., Rostoks, N., Ramsay, L., Druka, A., Stein, N., Svensson, J. T., Wanamaker, S. I., et al. (2009) · BMC Genomics · doi:10.1186/1471-2164-10-582 · Cited by 623 · see citing works
Møller, I. S., Gilliham, M., Jha, D., Mayo, G. M., Roy, S. J., Coates, J. C., Haseloff, J. P., & Tester, M. A. (2009) · The Plant Cell · doi:10.1105/tpc.108.064568 · Cited by 563 · see citing works
James, R. A., Blake, C., Byrt, C. S., & Munns, R. (2011) · Journal of Experimental Botany · doi:10.1093/jxb/err003 · Cited by 489 · see citing works
Root Plasma Membrane Transporters Controlling K+/Na+ Homeostasis in Salt-Stressed Barley
Chen, Z., Pottosin, I. I., Cuin, T. A., Fuglsang, A. T., Tester, M. A., Jha, D., Jazo, I. Z., Zhou, M., Palmgren, M. B., Newman, I. A., et al. (2007) · PLANT PHYSIOLOGY · doi:10.1104/pp.107.110262 · Cited by 487 · see citing works
Comadran, J., Kilian, B., Russell, J., Ramsay, L., Stein, N., Ganal, M. W., Shaw, P. D., Bayer, M., Thomas, W. T. B., Marshall, D., et al. (2012) · Nature Genetics · doi:10.1038/ng.2447 · Cited by 485 · see citing works
scatterplot3d- AnRPackage for Visualizing Multivariate Data
Ligges, U., & Mächler, M. (2003) · Journal of Statistical Software · doi:10.18637/jss.v008.i11 · Cited by 451 · see citing works
Drought and salt tolerances in wild relatives for wheat and barley improvement
Nevo, E., & Chen, G. (2009) · Plant Cell & Environment · doi:10.1111/j.1365-3040.2009.02107.x · Cited by 432 · see citing works
Rice OsHKT2;1 transporter mediates large Na+ influx component into K+‐starved roots for growth
Horie, T., Costa, A., Kim, T. H., Han, M. J., Horie, R., Leung, H., Miyao, A., Hirochika, H., An, G., & Schroeder, J. I. (2007) · The EMBO Journal · doi:10.1038/sj.emboj.7601732 · Cited by 402 · see citing works
Sodium transport and HKT transporters: the rice model
Garcíadeblas, B., Senn, M. E., Bañuelos, M. A., et al. (2003) · The Plant Journal · doi:10.1046/j.1365-313x.2003.01764.x · Cited by 399 · see citing works
HKT1;5-Like Cation Transporters Linked to Na + Exclusion Loci in Wheat, Nax2 and Kna1
Byrt, C. S., Platten, J. D., Spielmeyer, W., James, R. A., Lagudah, E. S., Dennis, E. S., Tester, M. A., & Munns, R. (2007) · PLANT PHYSIOLOGY · doi:10.1104/pp.106.093476 · Cited by 398 · see citing works
Why does salinity pose such a difficult problem for plant breeders?
Flowers, T. J., & Flowers, S. A. (2005) · Agricultural Water Management · doi:10.1016/j.agwat.2005.04.015 · Cited by 382 · see citing works
Nomenclature for HKT transporters, key determinants of plant salinity tolerance
Platten, J. D., Cotsaftis, O., Berthomieu, P., Bohnert, H. J., Davenport, R. J., Fairbairn, D. J., Horie, T., Leigh, R. A., Lin, H.-X., Sheng, L., et al. (2006) · Trends in Plant Science · doi:10.1016/j.tplants.2006.06.001 · Cited by 371 · see citing works
Physiological Characterization of Two Genes for Na+ Exclusion in Durum Wheat, Nax1 and Nax2
James, R. A., Davenport, R. J., & Munns, R. (2006) · PLANT PHYSIOLOGY · doi:10.1104/pp.106.086538 · Cited by 368 · see citing works
High-affinity potassium and sodium transport systems in plants
Rodríguez‐Navarro, A., & Rubio, F. (2006) · Journal of Experimental Botany · doi:10.1093/jxb/erj068 · Cited by 358 · see citing works
Genome-wide association mapping of salinity tolerance in rice (Oryza sativa)
Kumar, V., Singh, A., Mithra, S. V. A., Krishnamurthy, S. L., Parida, S. K., Jain, S., Tiwari, K. K., Kumar, P., Rao, A. R., Sharma, S. K., et al. (2015) · DNA Research · doi:10.1093/dnares/dsu046 · Cited by 328 · see citing works
Control of Sodium Transport in Durum Wheat
Davenport, R. J., James, R. A., Zakrisson-Plogander, A., Tester, M. A., & Munns, R. (2005) · PLANT PHYSIOLOGY · doi:10.1104/pp.104.057307 · Cited by 326 · see citing works
Improving salt tolerance of wheat and barley: future prospects
Colmer, T. D., Munns, R., & Flowers, T. J. (2005) · Australian Journal of Experimental Agriculture · doi:10.1071/ea04162 · Cited by 310 · see citing works
Véry, A., Nieves‐Cordones, M., Daly, M., Khan, I. H., Fizames, C., & Sentenac, H. (2014) · Journal of Plant Physiology · doi:10.1016/j.jplph.2014.01.011 · Cited by 301 · see citing works
Over‐expression of an Na + ‐ and K + ‐permeable HKT transporter in barley improves salt tolerance
Ahmad, M. A., Oomen, R. J. F. J., Isayenkov, S. V., Sentenac, H., Maathuis, F. J. M., & Véry, A. (2011) · The Plant Journal · doi:10.1111/j.1365-313x.2011.04701.x · Cited by 300 · see citing works
Natural Variants of AtHKT1 Enhance Na+ Accumulation in Two Wild Populations of Arabidopsis
Rus, A. M., Baxter, I. R., Balasubramaniam, M., Gustin, J., Lahner, B., Yakubova, E., & Salt, D. E. (2006) · PLoS Genetics · doi:10.1371/journal.pgen.0020210 · Cited by 299 · see citing works
Smart Engineering of Genetic Resources for Enhanced Salinity Tolerance in Crop Plants
Arzani, A., & Ashraf, M. (2016) · Critical Reviews in Plant Sciences · doi:10.1080/07352689.2016.1245056 · Cited by 286 · see citing works
Tibet is one of the centers of domestication of cultivated barley
Dai, F., Nevo, E., Wu, D., et al. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1215265109 · Cited by 281 · see citing works
Rapid and tissue-specific changes in ABA and in growth rate in response to salinity in barley leaves
Fricke, W. (2004) · Journal of Experimental Botany · doi:10.1093/jxb/erh117 · Cited by 261 · see citing works
Muñoz‐Amatriaín, M., Cuesta‐Marcos, A., Endelman, J. B., Comadran, J., Bonman, J. M., Bockelman, H. E., Chao, S., Russell, J., Waugh, R., Hayes, P. M., et al. (2014) · PLoS ONE · doi:10.1371/journal.pone.0094688 · Cited by 235 · see citing works
The Na+ transporter, Ta HKT 1;5‐D, limits shoot Na+ accumulation in bread wheat
Byrt, C. S., Xu, B., Krishnan, M., Lightfoot, D. J., Athman, A., Jacobs, A. K., Watson‐Haigh, N. S., Plett, D., Munns, R., Tester, M. A., et al. (2014) · The Plant Journal · doi:10.1111/tpj.12651 · Cited by 228 · see citing works
Diversity in Expression Patterns and Functional Properties in the Rice HKT Transporter Family
Jabnoune, M., Espéout, S., Mieulet, D., et al. (2009) · PLANT PHYSIOLOGY · doi:10.1104/pp.109.138008 · Cited by 219 · see citing works
Suzuki, K., Yamaji, N., Costa, A., Okuma, E., Kobayashi, N. I., Kashiwagi, T., Katsuhara, M., Wang, C., Tanoi, K., Murata, Y., et al. (2016) · BMC Plant Biology · doi:10.1186/s12870-016-0709-4 · Cited by 217 · see citing works
Platten, J. D., Egdane, J. A., & Ismail, A. M. (2013) · BMC Plant Biology · doi:10.1186/1471-2229-13-32 · Cited by 214 · see citing works
Chen, C.-Z., Lv, X.-F., Li, J., Yi, H.-Y., & Gong, J. (2012) · PLANT PHYSIOLOGY · doi:10.1104/pp.112.199257 · Cited by 200 · see citing works
Huang, S., Spielmeyer, W., Lagudah, E. S., & Munns, R. (2008) · Journal of Experimental Botany · doi:10.1093/jxb/ern033 · Cited by 195 · see citing works
Cotsaftis, O., Plett, D., Shirley, N. J., Tester, M. A., & Hrmová, M. (2012) · PLoS ONE · doi:10.1371/journal.pone.0039865 · Cited by 194 · see citing works
Improved Salinity Tolerance of Rice Through Cell Type-Specific Expression of AtHKT1;1
Plett, D., Safwat, G., Gilliham, M., Møller, I. S., Roy, S. J., Shirley, N. J., Jacobs, A. K., Johnson, A. A. T., & Tester, M. A. (2010) · PLoS ONE · doi:10.1371/journal.pone.0012571 · Cited by 162 · see citing works
Sahi, C., Singh, A., Blumwald, E., et al. (2006) · Physiologia Plantarum · doi:10.1111/j.1399-3054.2005.00610.x · Cited by 151 · see citing works
Asíns, M. J., Villalta, I., Mahmoud-Aly, M., et al. (2012) · Plant Cell & Environment · doi:10.1111/pce.12051 · Cited by 146 · see citing works
Association mapping of salt tolerance in barley (Hordeum vulgare L.)
Long, N. V., Dolstra, O., Malosetti, M., Kilian, B., Graner, A., Visser, R. G. F., & van der Linden, C. G. (2013) · Theoretical and Applied Genetics · doi:10.1007/s00122-013-2139-0 · Cited by 144 · see citing works
Shavrukov, Y. N., Gupta, N. K., Miyazaki, J., Baho, M. N., Chalmers, K. J., Tester, M. A., Langridge, P., & Collins, N. C. (2010) · Functional & Integrative Genomics · doi:10.1007/s10142-009-0153-8 · Cited by 140 · see citing works
Fan, Y., Shabala, S. N., Ma, Y., Xu, R., & Zhou, M. (2015) · BMC Genomics · doi:10.1186/s12864-015-1243-8 · Cited by 135 · see citing works
Kilian, B., Özkan, H., Kohl, J., von Haeseler, A., Barale, F., Deusch, O., Brandolini, A., Yücel, C., Martin, W. F., & Salamini, F. (2006) · Molecular Genetics and Genomics · doi:10.1007/s00438-006-0136-6 · Cited by 132 · see citing works
HKT Transporters—State of the Art
de Almeida, P. M. F., Katschnig, D., & de Boer, A. (2013) · International Journal of Molecular Sciences · doi:10.3390/ijms141020359 · Cited by 132 · see citing works
New allelic variants found in key rice salt‐tolerance genes: an association study
Negrão, S., Almadanim, M. C., Pires, I. S., Abreu, I. A., Marôco, J. P., Courtois, B., Gregorio, G. B., McNally, K. L., & Oliveira, M. M. (2012) · Plant Biotechnology Journal · doi:10.1111/pbi.12010 · Cited by 127 · see citing works
Identification of QTLs associated with salinity tolerance at late growth stage in barley
Xue, D., Huang, Y., Zhang, X., et al. (2009) · Euphytica · doi:10.1007/s10681-009-9919-2 · Cited by 110 · see citing works
Witzel, K., Weidner, A., Surabhi, G., et al. (2009) · Plant Cell & Environment · doi:10.1111/j.1365-3040.2009.02071.x · Cited by 98 · see citing works
QTLs for stomatal and photosynthetic traits related to salinity tolerance in barley
Liu, X., Fan, Y., Mak, M., Babla, M. H., Holford, P., Wang, F., Chen, G. J., Scott, G., Wang, G., Shabala, S. N., et al. (2017) · BMC Genomics · doi:10.1186/s12864-016-3380-0 · Cited by 98 · see citing works
Quantitative trait loci for salinity tolerance in barley (Hordeum vulgare L.)
Zhou, G., Johnson, P., Ryan, P. R., et al. (2011) · Molecular Breeding · doi:10.1007/s11032-011-9559-9 · Cited by 84 · see citing works
The Rice Monovalent Cation Transporter OsHKT2;4: Revisited Ionic Selectivity
Sassi, A., Mieulet, D., Khan, I. H., et al. (2012) · PLANT PHYSIOLOGY · doi:10.1104/pp.112.194936 · Cited by 72 · see citing works
Mapping Salinity Tolerance during Arabidopsis thaliana Germination and Seedling Growth
DeRose-Wilson, L., & Gaut, B. S. (2011) · PLoS ONE · doi:10.1371/journal.pone.0022832 · Cited by 70 · see citing works
Zhang, A., Liu, D., Hua, C., et al. (2016) · PLoS ONE · doi:10.1371/journal.pone.0168367 · Cited by 70 · see citing works
Fan, Y., Zhou, G., Shabala, S. N., Chen, Z., Cai, S., Li, C., & Zhou, M. (2016) · Frontiers in Plant Science · doi:10.3389/fpls.2016.00946 · Cited by 66 · see citing works
Two Novel Flavin-Containing Monooxygenases Involved in Biosynthesis of Aliphatic Glucosinolates
Kong, W., Li, J., Yu, Q., et al. (2016) · Frontiers in Plant Science · doi:10.3389/fpls.2016.01292 · Cited by 52 · see citing works
Jülke, S., & Ludwig‐Müller, J. (2015) · Plants · doi:10.3390/plants5010002 · Cited by 49 · see citing works
United Arab Emirates Keys to Soil Taxonomy
Shahid, S. A., Abdelfattah, M. A., Wilson, M. A., et al. (2014) · Springer eBooks · doi:10.1007/978-94-007-7420-9 · Cited by 37 · see citing works
Siahsar, B. A., & Narouei, M. (2010) · Journal of Food Agriculture & Environment · Cited by 36 · see citing works
Amar, S. B., Brini, F., Sentenac, H., et al. (2013) · Journal of Experimental Botany · doi:10.1093/jxb/ert361 · Cited by 31 · see citing works
QTL mapping for salt tolerance in barley at seedling growth stage
Ahmadi-Ochtapeh, H., Soltanloo, H., Ramezanpour, S. S., et al. (2015) · Biologia Plantarum · doi:10.1007/s10535-015-0496-z · Cited by 21 · see citing works
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Karunarathne, S. D., Han, Y., Zhang, X.-Q., Zhou, G., Hill, C. B., Chen, K., Angessa, T., & Li, C. (2020) · Frontiers in Plant Science · doi:10.3389/fpls.2020.571912 · Cited by 34 · see citing works
Karunarathne, S., Walker, E., Sharma, D., Li, C., & Han, Y. (2023) · Journal of Zhejiang University-SCIENCE B · doi:10.1631/jzus.b2200552 · Cited by 18 · see citing works
“Several barley genes have been exploited to ad‐ dress different stress conditions (Zhou et al., 2016; Hazzouri et al., 2018; Karunarathne et al., 2020; Mwando et al., 2020), including Hordeum vulgare aleurone 1 ( HVA1 ) for drought, high-affinity K + trans‐ porter 1;1 ( HKT1;1 ) for salinity, and…”
- Authors
- Hazzouri, K. M., Khraiwesh, B., Amiri, K. M. A., Pauli, D., Blake, T., Shahid, M., Mullath, S. K., Nelson, D., Mansour, A. L., Salehi-Ashtiani, K., Purugganan, M., & Masmoudi, K.
- Affiliations
- New York University Abu Dhabi (AE)
- United Arab Emirates University (AE)
- Cornell University (US)
- Montana State University (US)
- International Center for Biosaline Agriculture (AE)
- Corresponding author
- Hazzouri, Khaled M.
- Masmoudi, Khaled
- ORCID
- Venue
- Frontiers in Plant Science
- Publisher
- Frontiers Media SA
- Published
- 2018-02-19
- Volume
- 9
- Pages
- 156-156
- ISSN
- 1664-462X (ISSN-L)
- PMC
- PMC5826053
- OpenAlex
- W2793727353
- Cited by
- 91
- References
- 77
- Citations by year
- 2026: 11 · 2025: 4 · 2024: 8 · 2023: 10 · 2022: 17 · 2021: 14 · 2020: 14 · 2019: 11 · 2018: 1
- FWCI
- 8.85
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Frontiers in Plant Science (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- United Arab Emirates University (31F096)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Wheat and Barley Genetics and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Mapping and Diversity in Plants and Animals
- Keywords
- HKT1, barley, salt tolerance, salt stress, sodium accumulation, ion homeostasis, xylem loading, chromosome 4, gene expression, QTL mapping
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