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Cited by 17Carcinogens and Genotoxicity AssessmentAgricultural safety and regulationsFood Allergy and Anaphylaxis Research

Multi-Level Safety Studies of Anti Fel d 1 IgY Ingredient in Cat Food

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Matulka, Ray A., Thompson, Larry, Corley, David·2020·Frontiers in Veterinary Science
DOI 10.3389/fvets.2019.00477PMC PMC6960183
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Abstract

Chickens exposed to antigens produce IgY antibodies, similar in structure to mammalian IgG. Hens exposed with an allergen produced by cats (Fel d 1) results in production of anti-Fel d 1 specific IgY (AFD1), which is naturally concentrated in egg yolk. A chicken egg product ingredient containing AFD1 was evaluated for safety in a 26-week randomized, controlled, blinded tolerance study in cats and in vitro for mutagenic and genotoxic effects. The in vivo study was conducted with groups fed kibble containing 0, 7, 39, or 66 ppm AFD1. Parameters examined included: clinical observations, body weights, food consumption, serum chemistry, hematology, blood coagulation, urinalyses, and mortality and morbidity checks. AFD1 was evaluated for potential mutagenic effects utilizing the bacterial reverse mutation assay at concentrations of up to 2.78 ppm and for potential structural chromosomal aberrations at up to 3ppm using human peripheral blood lymphocytes. After 6-months of feeding to cats, there were no significant differences between control and any test groups in any parameters analyzed. No significant increases in mutations or chromosomal aberrations were observed in tests with or without S9. These studies show AFD1 was well-tolerated in cats at levels tested and does not induce mutagenic or chromosomal aberrations under study conditions.

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  • In cat feeding experiments, oral administration of anti-Fel d 1 IgY antibodies led to a 20-47% reduction in salivary Fel d 1 levels.

    “In cat feeding experiments, oral administration of anti-Fel d 1 IgY antibodies led to a 20–47% reduction in salivary Fel d 1 levels [127,128].”

    From Advance in Managing Indoor Cat Allergen Proteins: Molecular Insights, Detection, and Control (2025) · cites this paper as [128]

    The Conclusions (page unavailable) report that feeding cats anti-Fel d 1 IgY significantly reduced active salivary Fel d 1, and that 86% of treated cats in one trial had a reduction of at least 20%. The paper does not report a 20–47% reduction range; its 20% figure is a threshold met by individual cats, not the lower bound of a reported range. (LLM, conf=0.88)

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Sorted by citations

Authors
Matulka, R. A., Thompson, L., & Corley, D.
Affiliations
Nestlé (United States) (US)
Corresponding author
Matulka, Ray A.
ORCID
Matulka, Ray A.
Venue
Frontiers in Veterinary Science
Publisher
Frontiers Media SA
Published
2020-01-08
Volume
6
Pages
477-477
ISSN
2297-1769 (ISSN-L)
DOI
10.3389/fvets.2019.00477
PMC
PMC6960183
OpenAlex
W2995375199
Cited by
17
References
1
Citations by year
2026: 2 · 2025: 3 · 2024: 4 · 2023: 3 · 2021: 2 · 2020: 2 · 2019: 1
FWCI
0.60
Type
journal article
Language
en
Open access
Licence
Locations
  • Frontiers in Veterinary Science (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural safety and regulations
  • Health Sciences › Medicine › Immunology and Allergy › Food Allergy and Anaphylaxis Research
Keywords
Fel d 1, feline allergens, cat food, chicken egg yolk, food safety, tolerance study, mutagenicity, genotoxicity, chromosomal abnormalities, Ames test

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