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Cited by 7RNA Research and SplicingProtein Kinase Regulation and GTPase SignalingDevelopmental Biology and Gene Regulation

Expression of DOCK9 and DOCK11 Analyzed with Commercial Antibodies: Focus on Regulation of Mutually Exclusive First Exon Isoforms

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Parrado, Antonio·2020·Antibodies
DOI 10.3390/antib9030027PMC PMC7551865
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Abstract

Dedicators of cytokinesis 9 and 11 (DOCK9 and DOCK11) are members of the dedicator of cytokinesis protein family encoding the guanosine nucleotide exchange factors for Rho GTPases. Together with DOCK10, they constitute the DOCK-D or Zizimin subfamily. Two alternative full-length amino terminal isoforms of DOCK9 are known, which we will call DOCK9.1 and DOCK9.2. In order to investigate the relevance of the presence of the alternative first exon isoforms within this family, and to lay the groundwork for future studies that seek to investigate their potential role as biomarkers of disease, the expression levels of DOCK9 and DOCK11 were measured by qRT-PCR in 26 human tissues and 23 human cell lines, and by Western blot analysis, using commercial antibodies in cell lines. DOCK9.1 and DOCK9.2 were widely distributed. High levels of expression of both isoforms were found in the lungs, placenta, uterus, and thyroid gland. However, only DOCK9.1 was significantly expressed in the neural and hematopoietic tissues. The unique first exon form of DOCK11 was highly expressed in hematopoietic tissues, such as the peripheral blood leukocytes, spleen, thymus, or bone marrow, and in others such as the lungs, placenta, uterus, or thyroid gland. In contrast to tissues, the expression of DOCK9.1 and DOCK9.2 differed from one another and also from total DOCK9 in cell lines, suggesting that the amino terminal isoforms of DOCK9 may be differentially regulated. This study demonstrates the usefulness of antibodies in investigating the regulation of the expression of DOCK9.1, total DOCK9, and DOCK11.

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  • DOCK9 is widely and highly expressed throughout the body, including the brain; antibodies against two isoforms of DOCK9 indicate differing expression profiles.

    “It is widely and highly expressed throughout the body, including the brain; however, antibodies against two isoforms of DOCK9 indicated differing expression profiles (121).”

    From RHO to the DOCK for GDP disembarking: Structural insights into the DOCK GTPase nucleotide exchange factors (2021) · cites this paper as [121]

    The Introduction reports high DOCK9 mRNA expression in several tissues, including the brain, and the Abstract describes the isoforms as widely distributed; it does not say expression is high throughout the body. Results §3.3 reports differing profiles from two antibodies, but one detects DOCK9.1 while the other detects a region common to DOCK9 isoforms—not DOCK9.2 specifically. Our copy provides no page numbers for these passages. (LLM, conf=0.88)

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Sorted by citations

Authors
Parrado, A.
Affiliations
Instituto Murciano de Investigación Biosanitaria (ES)
Corresponding author
Parrado, Antonio
ORCID
Parrado, Antonio
Venue
Antibodies
Publisher
MDPI AG
Published
2020-06-27
Volume
9
Issue
3
Pages
27
ISSN
2073-4468 (ISSN-L)
DOI
10.3390/antib9030027
PMC
PMC7551865
OpenAlex
W3037414018
Cited by
7
References
1
Citations by year
2026: 1 · 2025: 1 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 2
FWCI
0.32
Type
journal article
Language
en
Open access
Licence
Locations
  • Antibodies (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Instituto de Salud Carlos III (PI10/01226)
  • Fundación Séneca de la Región de Murcia (08721/PI/08)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords
Rho GTPase guanine nucleotide exchange factor, tissue expression, human cell lines, antibody validation, hematopoietic tissue, nervous tissue

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