Cited by 25Atherosclerosis and Cardiovascular DiseasesCardiovascular, Neuropeptides, and Oxidative Stress ResearchAntimicrobial Peptides and Activities

Cathelicidin Modulates Vascular Smooth Muscle Cell Phenotypic Switching through ROS/IL-6 Pathway

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Dong, Xiaoliang, Wu, Di, Zhang, Yihan, Jia, Lingling, Pan, Xiaohua, Sun, Jia, Pan, Li-Long·2020·Antioxidants

Abstract

Vascular smooth muscle cells (VSMC) are stromal cells of the blood vessels and their differentiation is thought to be essential during atherosclerosis. Cathelicidin-related antimicrobial peptides (CRAMP) are suggested to play a role in the development of atherosclerosis. Even so, the relationship of CRAMP and VSMC remains unclear. The present study was to determine whether CRAMP regulates VSMC phenotypic transformation and underlying mechanisms. We demonstrated that CRAMP could reverse platelet-derived growth factor-BB (PDGF-BB)-induced VSMC phenotypic transformation, evidencing by increasing α-smooth muscle actin (α-SMA), smooth muscle 22α (SM22α) and decreasing of proliferation and migration. Further studies showed that CRAMP inhibited nuclear factor κB (NF-κB)-induced autocrine of interleukin-6 (IL-6), which further activated of janus kinase 2 (JAK2)/signal transducer and activator 3 (STAT3). Meanwhile, our data showed that CRAMP can significantly inhibit PDGF-BB enhanced intracellular reactive oxygen species (ROS) level which further affected the NF-κB signaling pathway, indicating that CRAMP can regulate the phenotypic transformation of VSMC by regulating oxidative stress. These results indicated that CRAMP regulated the differentiation of VSMC by inhibiting ROS-mediated IL-6 autocrine, suggesting that targeting CRAMP is a potential avenue for regulating the differentiation of VSMC and treatment of atherosclerosis.

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  • The human cathelicidin may activate ROS/NF-κβ/IL-6 and regulate oxidation signaling in cardiovascular diseases like atherosclerosis.

    “Other authors have suggested the role of the human cathelicidin on the activation of ROS/NF-κβ/IL-6 and propose that it could regulate oxidation signaling in cardiovascular diseases like atherosclerosis [184].”

    From The Role of Reactive Species on Innate Immunity (2022) · cites this paper as [184]

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Authors
Dong, X., Wu, D., Zhang, Y., Jia, L., Pan, X., Sun, J., & Pan, L.-L.
Affiliations
Jiangnan University (CN)
Corresponding author
  • Sun, Jia
  • Pan, Li-Long
Venue
Antioxidants
Publisher
MDPI AG
Published
2020-06-05
Volume
9
Issue
6
Pages
491
ISSN
2076-3921 (ISSN-L)
OpenAlex
W3033727401
Cited by
25
References
58
Citations by year
2026: 3 · 2025: 6 · 2024: 5 · 2023: 1 · 2022: 4 · 2021: 5 · 2020: 1
FWCI
0.96
Type
journal article
Language
en
Open access
Locations
  • Antioxidants (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Natural Science Foundation of China (Nos. 31571166; 81771703; 81471557; 81671565; 81902706)
  • Jiangsu Student’s Platform for innovation and entrepreneurship training program (2019167Y)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Atherosclerosis and Cardiovascular Diseases
  • Health Sciences › Medicine › Surgery › Cardiovascular, Neuropeptides, and Oxidative Stress Research
  • Life Sciences › Immunology and Microbiology › Microbiology › Antimicrobial Peptides and Activities
Keywords
cathelicidin-related antimicrobial peptide, vascular smooth muscle cells, phenotypic switching, atherosclerosis, PDGF-BB, reactive oxygen species, interleukin-6, NF-κB signaling, JAK2/STAT3 signaling, oxidative stress, smooth muscle cell proliferation and migration, alpha-smooth muscle actin

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