Cited by 31Seed and Plant BiochemistryPhytochemicals and Antioxidant ActivitiesFood Science and Nutritional Studies

Antioxidant and Cytoprotective Effect of Quinoa (Chenopodium quinoa Willd.) with Pressurized Hot Water Extraction (PHWE)

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Ong, Eng Shi, Pek, Charlene Jia Ning, Tan, Joseph Choon Wee, Leo, Chen Huei·2020·Antioxidants

Abstract

Quinoa is widely noted for its nutritional value. The seed is the main edible part of the plant and exists in at least three different colors: white, red and black. This study utilized a pressurized hot water extraction (PHWE) for the extraction of phytochemicals from quinoa. Chemical fingerprints with LC/UV and LC/MS using a targeted approach and pattern recognition tools were used to evaluate the quinoa extracts. The antioxidant properties for various types of quinoa were evaluated using DPPH assay, ABTS assay and the cytoprotective effect of quinoa extracts were investigated in HMEC-1 cell line. Distinctive chemical profiles obtained from black and red quinoa were well correlated with the antioxidant activities and cytoprotective effects. The combination of PHWE, chemical standardization with LC/UV and LC/MS, pattern recognition tools and biological assay provided an approach for the evaluation and eventual production of quinoa extracts for functional food.

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  1. [34]ICH Expert Working Group ICH Harmonised Tripartite Guideline Impurities in New Drug Products Q3B(R2) Int. Conf. Harmon. Tech. Requir. Regist. Pharm. Hum. Use 2006 Available online: http://academy.gmp-compliance.org/guidemgr/files/ICH%20Q3B%20R2 (accessed on 1 November 2020)

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Authors
Ong, E. S., Pek, C. J. N., Tan, J. C. W., & Leo, C. H.
Affiliations
Singapore University of Technology and Design (SG)
Corresponding author
Ong, Eng Shi
Venue
Antioxidants
Publisher
MDPI AG
Published
2020-11-11
Volume
9
Issue
11
Pages
1110
ISSN
2076-3921 (ISSN-L)
OpenAlex
W3100126728
Cited by
31
References
39
Citations by year
2026: 2 · 2025: 7 · 2024: 4 · 2023: 9 · 2022: 6 · 2021: 3
FWCI
2.92
Type
journal article
Language
en
Open access
Locations
  • Antioxidants (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
SUTD-MIT International Design Center Pilot Research Grant (IDG11900102) ((IDG11900102))
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Seed and Plant Biochemistry
  • Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
  • Health Sciences › Nursing › Nutrition and Dietetics › Food Science and Nutritional Studies
Keywords
quinoa, subcritical water extraction, antioxidant activity, cytoprotection, phytochemicals, HMEC-1 cells, chemical fingerprinting, LC-MS, functional foods

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