Cited by 42Insect Utilization and EffectsFood and Agricultural SciencesProtein Hydrolysis and Bioactive Peptides

Functional, Antioxidant, and Anti-Inflammatory Properties of Cricket Protein Concentrate (Gryllus assimilis)

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Quinteros, María Fernanda, Martínez, Jenny, Barrionuevo, Alejandra, Rojas, Marcelo, Carrillo, Wilman·2022·Biology

Abstract

Edible insects can represent an alternative to obtain high-quality proteins with positive biological properties for human consumption. Cricket flour (Gryllus assimilis) was used to obtain cricket protein concentrate (CPC) using pHs (10.0 and 12.0) of extraction and pHs (3.0, 4.0, 5.0, and 6.0) of isoelectric precipitation (pI). Protein content, water and oil absorption capacity, protein solubility, antioxidant, and anti-inflammatory activities were determined. In addition, the protein profile was characterized by electrophoresis and the in vitro CPC digestibility was evaluated. Cricket flour presented 45.75% of protein content and CPC 12–5.0 presented a value of 71.16% protein content using the Dumas method. All samples were more soluble at pH 9.0 and 12.0. CPC 12–3.0 presented a percentage of water-binding capacity (WBC) of 41.25%. CPC 12–6.0 presented a percentage of oil-binding capacity (OBC) of 72.93%. All samples presented a high antioxidant and anti-inflammatory activity. CPC 12–4.0 presented a value FRAP of 70,034 umol trolox equivalents (TE)/g CPC, CPC 12–6.0 presented a value ABTS of 124,300 umol TE/g CPC and CPC 10–3.0 presented a DPPH value of 68,009 umol TE/g CPC. CPC 10–6.0 and CPC 12–6.0 presented high anti-inflammatory activity, with values of 93.55% and 93.15% of protection, respectively. CPCs can be used as functional ingredients in the food industry for their excellent functional and biological properties.

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Crossref · 10.3390/biology11050776

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  • The functional properties were evaluated following the methodology proposed by Quinteros and Thirumuruga.

    “The functional properties of the proteins evaluated were protein solubility, water absorption capacity, oil absorption capacity, foaming capacity, and foam stability, following the methodology proposed by Quinteros [17] and Thirumuruga [18].”

    From Functional and Bioactive Characterization of Hemp Cake Proteins and Polyphenols from Non-Psychoactive Cannabis sativa (2025) · cites this paper as Quinteros [17]

    Section 2.6 (page unavailable) describes methods for protein solubility, water absorption capacity, and oil absorption capacity, supporting the citation to Quinteros for those evaluations. It does not describe foaming capacity or foam stability, and it credits the methodology it uses to Pazmiño et al. (2018). The separate citation to Thirumuruga is not assessed here. (LLM, conf=0.86)

    Experimental — accuracy not yet measured
  • The anti-inflammatory activity was evaluated using the erythrocyte membrane stabilization method described by Quinteros et al., with slight modifications.

    “The anti-inflammatory activity was evaluated using protein concentrates derived from hemp cake, following the erythrocyte membrane stabilization method described by Quinteros et al. [17], with slight modifications.”

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Authors
Quinteros, M. F., Martínez, J., Barrionuevo, A., Rojas, M., & Carrillo, W.
Affiliations
  • Universidad Técnica de Cotopaxi (EC)
  • Universidad Estatal de Bolívar (EC)
  • Universitat Politècnica de València (ES)
Corresponding author
Carrillo, Wilman
Venue
Biology
Publisher
MDPI AG
Published
2022-05-20
Volume
11
Issue
5
Pages
776
ISSN
2079-7737 (ISSN-L)
OpenAlex
W4280509624
Cited by
41
References
58
Citations by year
2026: 15 · 2025: 13 · 2024: 8 · 2023: 4 · 2022: 1
FWCI
4.73
Type
journal article
Language
en
Open access
Locations
  • Biology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect Utilization and Effects
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Food and Agricultural Sciences
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Hydrolysis and Bioactive Peptides
Keywords
Gryllus assimilis, cricket flour, isoelectric precipitation, extraction pH, protein solubility, water holding capacity, oil absorption capacity, antioxidant activity, anti-inflammatory activity, in vitro digestibility, functional food ingredients, protein profile

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