Identification of Phytochemical Compounds, Functional Properties and Antioxidant Activity of Germinated Purple Corn Protein Concentrate and Its Gastrointestinal Hydrolysates
Open accessAbstract
Purple corn seeds germinated at 25 °C and 35 °C for 5 days were used to obtain purple corn protein concentrate (PCPC25 and PCPC35). PCPC25 and PCPC35 were subject to gastrointestinal hydrolysis. PCPC and its hydrolysates were used to evaluate their functional and biological properties. Total flavonoid content (TFC), total flavonol content (TFLC), total anthocyanin content (TAC) and total proanthocyanin (TAP) were determined. The polyphenols and flavonoids were identified by UPLC-QDa. Protein solubility (PS), water and oil absorption capacity (WAC and OAC) were determined. The antioxidant activity was evaluated by the FRAP, ABTS and DPPH methods. PCPC35 showed the highest TFLC values (11,091.37 mg rutin equivalents (RE)/100 g dry weight DW), and TFL presented values of 7975.59 mg RE/100 g DW. The PCPCs presented better functional properties than the hydrolysates. PCPC25 presented a PS of 59.43%PS at pH 8.0, 27.77%WAC and 24.94%OAC. PCPC25 and PCPC35 showed high values of antioxidant activity. PCPC25 showed ABTS values (570.97 µmol trolox equivalents (TE)/g DW) and FRAP (772.85 µmol TE/g DW). PCPCs hydrolysates were less active with ABTS values (74.12 µmol TE/g DW) and FRAP (59.42 µmol TE/g DW).
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Total flavonoids were determined using the aluminum chloride colorimetric method.
“Total flavonoids were determined using the aluminum chloride colorimetric method [22].”
From Functional and Bioactive Characterization of Hemp Cake Proteins and Polyphenols from Non-Psychoactive Cannabis sativa (2025) · cites this paper as [22]
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- Authors
- Vilcacundo, E., Montalvo, V., Sanaguano, H., Moran, R., Carrillo, W., & García, A.
- Affiliations
- Universidad Estatal de Bolívar (EC)
- Departamento de Investigación, Universidad Estatal de Bolívar, Guaranda 020102, Bolívar, Ecuador
- University of Córdoba (ES)
- Universitat Politècnica de València (ES)
- Corresponding author
- García, Antón
- Venue
- Agronomy
- Publisher
- MDPI AG
- Published
- 2022-09-17
- Volume
- 12
- Issue
- 9
- Pages
- 2217
- ISSN
- 2073-4395 (ISSN-L)
- OpenAlex
- W4296312174
- Cited by
- 8
- References
- 70
- Citations by year
- 2026: 1 · 2025: 6 · 2023: 1
- FWCI
- 0.57
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Agronomy (publishedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Universidad de Córdoba Insitutional Repository (Universidad de Córdoba) (submittedVersion, cc-by)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Hydrolysis and Bioactive Peptides
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Life Sciences › Agricultural and Biological Sciences › Food Science › Food and Agricultural Sciences
- Keywords
- purple corn, seed germination, protein concentrate, proteolysis, flavonoids, flavonols, anthocyanins, proanthocyanidins, polyphenols, antioxidant activity, functional properties, protein solubility, water and oil absorption capacity, germination temperature
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