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Multiplex Assays in Allergy Diagnosis: Allergy Explorer 2 versus ImmunoCAP ISAC E112i
Abstract
ImmunoCAP ISAC E112i (ISAC) and Allergy Explorer 2 (ALEX2) detect specific immunoglobulin E (IgE) reactivity. Both multiplex assays contain molecular allergens and ALEX2 additionally includes allergen extracts and inhibitors that block the binding of IgE to cross-reacting carbohydrate determinants (CCDs). This study aimed to compare the performance of ISAC and ALEX2 by determining the IgE reactivity against allergen extracts and/or allergen components and by using qualitative, semiquantitative, and quantitative analyses of all comparable allergen components in sera from 216 participants recruited in South Tyrol/Italy. For extract sensitization in ALEX2, the analysis revealed negative corresponding allergen components in 18.4% and at least one positive corresponding allergen component in 81.6% of all cases. For ISAC, the corresponding results were 23.5% and 76.5% of cases, respectively. The ALEX2 CCD inhibitor eliminated CCD-positive signals detected by ISAC in 88.5% of cases. Based on sensitization values of 0.3–14.9 ISU or kUA/L, there was good agreement between ALEX2 and ISAC, although ALEX2 showed higher values than ISAC. The addition of allergen-extract tests in ALEX2 resulted in the detection of more sensitizations than with corresponding allergen components alone. In the range of <15 ISU or kUA/L, ALEX2 may be more effective in detecting sensitizations.
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“Unlike ISAC, ALEX includes cross-reactivity inhibition, which minimizes false positives from panallergens like profilins and CCDs (cross-reactive carbohydrate determinants) [201].”
From Pollen–Food Allergy Syndrome: Allergens, Clinical Insights, Diagnostic and Therapeutic Challenges (2024) · cites this paper as [201]
In the citing paper
“ISAC (Immuno Solid-Phase Allergen Chip; ThermoFisher Scientific, Waltham, MA, USA) is a multiplex diagnostic platform that tests for IgE to over 100 allergenic components simultaneously [201].”
From Pollen–Food Allergy Syndrome: Allergens, Clinical Insights, Diagnostic and Therapeutic Challenges (2024) · cites this paper as [201]
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Cited by
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Pollen–Food Allergy Syndrome: Allergens, Clinical Insights, Diagnostic and Therapeutic Challenges
Haidar, L., Bănărescu, C. F., Uța, C., Moldovan, S. I., Zimbru, E.-L., Zimbru, R.-I., Ciurariu, E., Georgescu, M., & Panaitescu, C. (2024) · Applied Sciences · doi:10.3390/app15010066 · Cited by 21
Metadata
- Authors
- Nösslinger, H., Mair, E., Oostingh, G. J., Ahlgrimm-Siess, V., Ringauf, A., & Lang, R.
- Venue
- Diagnostics
- Publisher
- MDPI AG
- Published
- 2024-05-08
- Volume
- 14
- Issue
- 10
- Pages
- 976
- ISSN
- 2075-4418
- PMC
- PMC11119049
- Cited by
- 21
- References
- 1
- Language
- en
- Funders
- South Tyrolean Fund for the Promotion of Scientific Research (SFPR) at the South Tyrolean Health Care Service (SABES)
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- Nösslinger, H., Mair, E., Oostingh, G. J., Ahlgrimm-Siess, V., Ringauf, A., & Lang, R. (2024). Multiplex Assays in Allergy Diagnosis: Allergy Explorer 2 versus ImmunoCAP ISAC E112i. Diagnostics. https://doi.org/10.3390/diagnostics14100976
