Cited by 53Biofuel production and bioconversionMicrobial Metabolic Engineering and BioproductionBiochemical and biochemical processes

Optimization and Scale-Up of Coffee Mucilage Fermentation for Ethanol Production

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Orrego, David, Zapata-Zapata, Arley, Kim, Daehwan·2018·Energies

Abstract

Coffee, one of the most popular food commodities and beverage ingredients worldwide, is considered as a potential source for food industry and second-generation biofuel due to its various by-products, including mucilage, husk, skin (pericarp), parchment, silver-skin, and pulp, which can be produced during the manufacturing process. A number of research studies have mainly investigated the valuable properties of brewed coffee (namely, beverage), functionalities, and its beneficial effects on cognitive and physical performances; however, other residual by-products of coffee, such as its mucilage, have rarely been studied. In this manuscript, the production of bioethanol from mucilage was performed both in shake flasks and 5 L bio-reactors. The use of coffee mucilage provided adequate fermentable sugars, primarily glucose with additional nutrient components, and it was directly fermented into ethanol using a Saccharomyces cerevisiae strain. The initial tests at the lab scale were evaluated using a two-level factorial experimental design, and the resulting optimal conditions were applied to further tests at the 5 L bio-reactor for scale up. The highest yields of flasks and 5 L bio-reactors were 0.46 g ethanol/g sugars, and 0.47 g ethanol/g sugars after 12 h, respectively, which were equal to 90% and 94% of the theoretically achievable conversion yield of ethanol.

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Crossref · 10.3390/en11040786

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Authors
Orrego, D., Zapata-Zapata, A., & Kim, D.
Affiliations
  • Purdue University West Lafayette (US)
  • School of Chemistry, Faculty of Science, National University of Colombia, Calle 59A N, Medellin 63-20, Colombia
Corresponding author
Kim, Daehwan
Venue
Energies
Publisher
MDPI AG
Published
2018-03-29
Volume
11
Issue
4
Pages
786
ISSN
1996-1073 (ISSN-L)
OpenAlex
W2794972811
Cited by
53
References
51
Citations by year
2026: 1 · 2025: 3 · 2024: 6 · 2023: 8 · 2022: 12 · 2021: 9 · 2020: 8 · 2019: 3 · 2018: 3
FWCI
2.30
Type
journal article
Language
en
Open access
Locations
  • Energies (publishedVersion, cc-by)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Purdue University
  • Universidad Nacional de Colombia
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Biochemical and biochemical processes
Keywords
coffee mucilage, bioethanol production, Saccharomyces cerevisiae, fermentation optimization, scale-up, coffee by-products, second-generation biofuels, glucose fermentation, ethanol yield, factorial design

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