Analysis of Multi-Biofuel Production during Cultivation of the Green Microalga Tetraselmis subscordiformis
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Research to date has mainly focused on the properties and efficiency of the production of selected, individual types of biofuels from microalgae biomass. There are not enough studies investigating the efficiency of the production of all energy sources synthesised by these microorganisms in a single technological cycle. The aim of this research was to determine the possibilities and efficiency of the production of hydrogen, bio-oil, and methane in the continuous cycle of processing T. subcordiformis microalgae biomass. This study showed it was feasible to produce these three energy carriers, but the production protocol adopted was not necessarily valuable from the energy gain standpoint. The production of bio-oil was found to be the least viable process, as bio-oil energy value was only 1.3 kWh/MgTS. The most valuable single process for microalgae biomass conversion turned out to be methane fermentation. The highest specific gross energy gain was found after applying a protocol combining biomass production, hydrogen biosynthesis, and subsequent methane production from T. subcordiformis biomass, which yielded a total value of 1891.4 kWh/MgTS. The direct methane fermentation of T. subcordiformis biomass enabled energy production at 1769.8 kWh/MgTS.
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The main energy carriers obtained from microalgae include biodiesel produced from the lipids accumulated in their cells, hydrogen produced by photobiological processes, biomethane produced by fermentation processes, and biomass that can be used by pyrolysis, gasification, or direct combustion technologies.
“The main energy carriers obtained from microalgae include biodiesel produced from the lipids accumulated in their cells, hydrogen produced by photobiological processes, biomethane produced by fermentation processes, and biomass that can be used by pyrolysis, gasification, or direct combustion technologies [29-31].”
From Multi-Sensing Monitoring of the Microalgae Biomass Cultivation Systems for Biofuels and Added Value Products Synthesis—Challenges and Opportunities (2025) · cites this paper as [29]
Cites
2 retracted
Microalgae: The Future Supply House of Biohydrogen and Biogas
Wang, K., Khoo, K. S., Chew, K. W., Selvarajoo, A., Chen, W.-H., Chang, J.-S., & Show, P. L. (2021) · Frontiers in Energy Research · doi:10.3389/fenrg.2021.660399 · Cited by 119 · see citing works
RetractedHu, R., Cao, Y., Chen, X., Zhan, J., Luo, G., Ngo, H. H., & Zhang, S. (2022) · Chemical Engineering Journal · doi:10.1016/j.cej.2022.137187 · Cited by 10 · see citing works
RetractedSiddiki, S. Y. A., Mofijur, M., Kumar, P. S., Ahmed, S. F., Inayat, A., Kusumo, F., Badruddin, I. A., Khan, T. M. Y., Nghiem, L. D., Ong, H. C., et al. (2021) · Fuel · doi:10.1016/j.fuel.2021.121782 · Cited by 466 · see citing works
Chew, K. W., Chia, S. R., Show, P. L., Yap, Y. J., Ling, T. C., & Chang, J.-S. (2018) · Journal of the Taiwan Institute of Chemical Engineers · doi:10.1016/j.jtice.2018.05.039 · Cited by 324 · see citing works
Wastewater based microalgal biorefinery for bioenergy production: Progress and challenges
Bhatia, S. K., Mehariya, S., Bhatia, R. K., Kumar, M., Pugazhendhi, A., Awasthi, M. K., Atabani, A. E., Kumar, G., Kim, W., Seo, S.-O., et al. (2020) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2020.141599 · Cited by 310 · see citing works
Biogas from microalgae: Technologies, challenges and opportunities
Zabed, H. M., Akter, S., Yun, J., Zhang, G., Zhang, Y., & Qi, X. (2019) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2019.109503 · Cited by 289 · see citing works
Role of Microalgae in Achieving Sustainable Development Goals and Circular Economy
Olabi, A. G., Shehata, N., Sayed, E. T., Rodriguez, C., Anyanwu, R. C., Russell, C., & Abdelkareem, M. A. (2023) · Science of The Total Environment · doi:10.1016/j.scitotenv.2022.158689 · Cited by 273 · see citing works
Muhammad, G., Alam, M. A., Mofijur, M., Jahirul, M. I., Lv, Y., Xiong, W., Ong, H. C., & Xu, J. (2021) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2020.110209 · Cited by 226 · see citing works
Two-stage photo-biological production of hydrogen by marine green alga Platymonas subcordiformis
Guan, Y., Deng, M., Yu, X., & Zhang, W. Y. (2004) · Biochemical Engineering Journal · doi:10.1016/j.bej.2003.10.006 · Cited by 192 · see citing works
A realistic scenario on microalgae based biodiesel production: Third generation biofuel
Ananthi, V., Raja, R., Carvalho, I. S., Brindhadevi, K., Pugazhendhi, A., & Arun, A. (2020) · Fuel · doi:10.1016/j.fuel.2020.118965 · Cited by 192 · see citing works
Bohutskyi, P., Betenbaugh, M. J., & Bouwer, E. J. (2014) · Bioresource Technology · doi:10.1016/j.biortech.2013.12.095 · Cited by 183 · see citing works
López-Sánchez, A., Silva-Gálvez, A. L., Aguilar-Juárez, Ó., Senés-Guerrero, C., Orozco-Nunnelly, D. A., Carrillo-Nieves, D., & Gradilla-Hernández, M. S. (2022) · Journal of Environmental Management · doi:10.1016/j.jenvman.2022.114612 · Cited by 180 · see citing works
Ge, S., Qiu, S., Tremblay, D., Viner, K., Champagne, P., & Jessop, P. G. (2018) · Chemical Engineering Journal · doi:10.1016/j.cej.2018.02.058 · Cited by 164 · see citing works
Biohydrogen production from microalgae—Major bottlenecks and future research perspectives
Nagarajan, D., Dong, C., Chen, C., Lee, D., & Chang, J. (2020) · Biotechnology Journal · doi:10.1002/biot.202000124 · Cited by 154 · see citing works
Hernández, D., Solana, M., Riaño, B., García-González, M. C., & Bertucco, A. (2014) · Bioresource Technology · doi:10.1016/j.biortech.2014.07.109 · Cited by 153 · see citing works
Microalgae in a global world: New solutions for old problems?
de Mendonça, H. V., Assemany, P. P., Abreu, M., do Couto, E. D. A., Maciel, A. M., Duarte, R. L., dos Santos, M. G. B., & dos Reis, A. J. D. (2020) · Renewable Energy · doi:10.1016/j.renene.2020.11.014 · Cited by 144 · see citing works
Guo, Z., Liu, Y., Guo, H., Yan, S., & Mu, J. (2013) · Journal of Environmental Sciences · doi:10.1016/s1001-0742(14)60632-x · Cited by 138 · see citing works
Anderson, A., Anbarasu, A., Pasupuleti, R. R., Manigandan, S., Praveenkumar, T. R., & Aravind Kumar, J. (2022) · Chemosphere · doi:10.1016/j.chemosphere.2022.133724 · Cited by 124 · see citing works
The role of oxygen regulation and algal growth parameters in hydrogen production via biophotolysis
Javed, M. A., Zafar, A. M., Hassan, A. A., Zaidi, A. A., Farooq, M., Badawy, A. M. E., Lundquist, T., Mohamed, M. M., & Al‐Zuhair, S. (2021) · Journal of environmental chemical engineering · doi:10.1016/j.jece.2021.107003 · Cited by 120 · see citing works
Wychen, S. V., Ramirez, K. J., & Laurens, L. M. L. (2014) · doi:10.2172/1118085 · Cited by 118 · see citing works
Aketo, T., Hoshikawa, Y., Nojima, D., Yabu, Y., Maeda, Y., Yoshino, T., Takano, H., & Tanaka, T. (2020) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2019.12.004 · Cited by 118 · see citing works
Wei, L., Huang, X., & Huang, Z. (2015) · Chinese Journal of Oceanology and Limnology · doi:10.1007/s00343-015-3346-0 · Cited by 97 · see citing works
Kuo, P., Illathukandy, B., Wu, W., & Chang, J. (2021) · Energy · doi:10.1016/j.energy.2021.120025 · Cited by 88 · see citing works
An overview on microalgae as renewable resources for meeting sustainable development goals
Oliveira, C. Y. B., Jacob, A., Nader, C., Oliveira, C. D. L., Matos, Â. P., Araújo, E. S., Shabnam, N., Ashok, B., & Gálvez, A. O. (2022) · Journal of Environmental Management · doi:10.1016/j.jenvman.2022.115897 · Cited by 83 · see citing works
Torri, C., Samorì, C., Adamiano, A., Fabbri, D., Faraloni, C., & Torzillo, G. (2011) · Bioresource Technology · doi:10.1016/j.biortech.2011.01.064 · Cited by 82 · see citing works
Goswami, R. K., Agrawal, K., Mehariya, S., & Verma, P. (2021) · Environmental Science and Pollution Research · doi:10.1007/s11356-021-16860-5 · Cited by 73 · see citing works
Chen, Z., Xie, Y., Qiu, S., Li, M., Yuan, W., & Ge, S. (2022) · Bioresource Technology · doi:10.1016/j.biortech.2022.127130 · Cited by 72 · see citing works
Bioethanol production from microalgae biomass at high-solids loadings
Condor, B. E., de Luna, M. D. G., Chang, Y., Chen, J.-H., Leong, Y. K., Chen, P., Chen, C., Lee, D., & Chang, J. (2022) · Bioresource Technology · doi:10.1016/j.biortech.2022.128002 · Cited by 68 · see citing works
Urban wastewater treatment by Tetraselmis sp. CTP4 (Chlorophyta)
Schulze, P. S. C., de Carvalho, C. F. M., Pereira, H., Gangadhar, K. N., Schüler, L., Santos, T., Varela, J. C. S., & Barreira, L. A. (2016) · Bioresource Technology · doi:10.1016/j.biortech.2016.10.027 · Cited by 66 · see citing works
Lakshmikandan, M., Wang, S., Murugesan, A., Saravanakumar, M., & Selvakumar, G. (2021) · Bioresource Technology · doi:10.1016/j.biortech.2021.125118 · Cited by 66 · see citing works
Microalgal-Based Bioenergy: Strategies, Prospects, and Sustainability
Choudhary, S., Tripathi, S., & Poluri, K. M. (2022) · Energy & Fuels · doi:10.1021/acs.energyfuels.2c02922 · Cited by 64 · see citing works
Ji, C., Yu, X., Chen, Z., Xue, S., Legrand, J., & Zhang, W. Y. (2011) · International Journal of Hydrogen Energy · doi:10.1016/j.ijhydene.2010.12.138 · Cited by 61 · see citing works
Ge, S., Brindhadevi, K., Xia, C., Khalifa, A. S., Elfasakhany, A., Unpaprom, Y., & Whangchai, K. (2022) · Fuel · doi:10.1016/j.fuel.2021.121898 · Cited by 59 · see citing works
Zhang, L., Liu, J., Li, D., Yang, Z., Wang, X., & Lin, R. (2022) · Energy Conversion and Management · doi:10.1016/j.enconman.2022.115475 · Cited by 55 · see citing works
Amit, Chandra, R., Ghosh, U. K., & Nayak, J. K. (2017) · Environmental Science and Pollution Research · doi:10.1007/s11356-017-9734-6 · Cited by 53 · see citing works
Enhanced microalgae biomass and lipid output for increased biodiesel productivity
Morais, K. C. C., Conceição, D., VARGAS, J. V. C., Mitchell, D. A., Mariano, A. B., Ordóñez, J. C., Galli-Terasawa, L. V., & Kava, V. M. (2020) · Renewable Energy · doi:10.1016/j.renene.2020.08.082 · Cited by 47 · see citing works
Dębowski, M., Kisielewska, M., Kazimierowicz, J., Rudnicka, A., Dudek, M., Romanowska‐Duda, Z., & Zieliński, M. (2020) · Energies · doi:10.3390/en13092186 · Cited by 46 · see citing works
Self-citationHarnessing the Algal Chloroplast for Heterologous Protein Production
Cutolo, E. A., Mandalà, G., Dall’Osto, L., & Bassi, R. (2022) · Microorganisms · doi:10.3390/microorganisms10040743 · Cited by 43 · see citing works
Mixotrophic cultivation of Platymonas subcordiformis
Xie, J., Zhang, Y., Li, Y., & Wang, Y. (2001) · Journal of Applied Phycology · doi:10.1023/a:1017532302360 · Cited by 42 · see citing works
Characterization of hydrogen production by Platymonas Subcordiformis in torus photobioreactor
Ji, C., Legrand, J., Pruvost, J., Chen, Z., & Zhang, W. Y. (2010) · International Journal of Hydrogen Energy · doi:10.1016/j.ijhydene.2010.02.085 · Cited by 41 · see citing works
Huang, X., Wei, L., Huang, Z., & Yan, J. (2013) · Journal of Applied Phycology · doi:10.1007/s10811-013-0056-x · Cited by 39 · see citing works
Grierson, S., Strezov, V., Bray, S., Mummacari, R., Danh, L. T., & Foster, N. R. (2011) · Energy & Fuels · doi:10.1021/ef2011222 · Cited by 39 · see citing works
Santos-Ballardo, D. U., Font, X., Sánchez, A., Barrena, R., Rossi, S., & Valdez-Ortíz, Á. (2015) · Waste Management & Research The Journal for a Sustainable Circular Economy · doi:10.1177/0734242x15572182 · Cited by 38 · see citing works
Qi, X., Wei, X., Yang, Z., Xie, T., Zhang, Y., Li, D., Pan, X., Liu, X., Zhang, X., & Yao, C. (2021) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2021.146687 · Cited by 32 · see citing works
Heo, S.-W., Ryu, B.-G., Nam, K., Kim, W., & Yang, J. (2015) · Bioprocess and Biosystems Engineering · doi:10.1007/s00449-015-1380-0 · Cited by 25 · see citing works
Ran, W., Qi, X., Pan, Y., Xie, T., Zhang, Y., & Yao, C. (2020) · Industrial & Engineering Chemistry Research · doi:10.1021/acs.iecr.0c00398 · Cited by 25 · see citing works
Dudek, M., Dębowski, M., Kazimierowicz, J., Zieliński, M., Quattrocelli, P., & Nowicka, A. (2022) · Sustainability · doi:10.3390/su141912085 · Cited by 25 · see citing works
Self-citationA review of biogas slurry treatment technology based on microalgae cultivation
Huang, L., Liu, J., Li, Q., Wang, C., Wu, K., Wang, C., Zhao, X., Yin, F., Liang, C., & Zhang, W. (2021) · Current Opinion in Environmental Science & Health · doi:10.1016/j.coesh.2021.100315 · Cited by 23 · see citing works
Zieliński, M., Dębowski, M., & Kazimierowicz, J. (2021) · Processes · doi:10.3390/pr9101772 · Cited by 19 · see citing works
Self-citationDębowski, M., Kisielewska, M., Zieliński, M., & Kazimierowicz, J. (2023) · Applied Sciences · doi:10.3390/app13137387 · Cited by 18 · see citing works
Self-citationGuo, Z., Li, Y., & Guo, H. (2017) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-017-2498-0 · Cited by 16 · see citing works
Chen, W., & Felix, C. B. (2024) · Energy · doi:10.1016/j.energy.2024.131677 · Cited by 16 · see citing works
Ran, C., Zhang, F., Sun, H., & Zhao, B. (2009) · Biotechnology and Bioprocess Engineering · doi:10.1007/s12257-008-0287-x · Cited by 13 · see citing works
Dębowski, M., Kazimierowicz, J., Nowicka, A., Dudek, M., & Zieliński, M. (2024) · Energies · doi:10.3390/en17061409 · Cited by 13 · see citing works
Self-citationMontoya, L. M. M., Arbeláez, A., Giraldo, N. D., & Atehortúa, L. (2024) · Journal of Applied Phycology · doi:10.1007/s10811-024-03208-y · Cited by 12 · see citing works
Dudek, M., Dębowski, M., Nowicka, A., Kazimierowicz, J., & Zieliński, M. (2022) · Resources · doi:10.3390/resources11030031 · Cited by 11 · see citing works
Self-citationGuo, Z., Li, Y., & Guo, H. (2015) · Biotechnology Letters · doi:10.1007/s10529-015-2008-9 · Cited by 10 · see citing works
Cyanobacteria as Renewable Sources of Bioenergy (Biohydrogen, Bioethanol, and Bio-Oil Production)
Sivaramakrishnan, R., & Incharoensakdi, A. (2021) · Springer eBooks · doi:10.1007/978-981-16-4873-1_19 · Cited by 10 · see citing works
Feki, F., Cherif, M., Masmoudi, M. A., Chamkha, M., Saadaoui, I., Das, P., & Sayadi, S. (2023) · Environmental Technology & Innovation · doi:10.1016/j.eti.2023.103478 · Cited by 7 · see citing works
Dębowski, M., Kazimierowicz, J., Nowicka, A., et al. (2024) · Preprints.org · doi:10.20944/preprints202403.0156.v1 · Cited by 5 · see citing works
Self-citationDudek, M., Nowicka, A., Zieliński, M., Kazimierowicz, J., & Dębowski, M. (2022) · International journal of energy and environmental engineering · doi:10.1007/s40095-022-00507-3 · Cited by 4 · see citing works
Self-citationMicroalgae: The challenges from harvest to the thermal gasification
Martins, M. F., Soares, R. B., & Gonçalves, R. F. (2022) · Elsevier eBooks · doi:10.1016/b978-0-323-90476-6.00007-8 · Cited by 2 · see citing works
Microalgae Biomass as an Alternative to Fossil Carbons
Ferreira, J. D., Martins, C. B., Assunção, M. F. G., & Santos, L. M. A. (2022) · CRC Press eBooks · doi:10.1201/9780429340543-7 · Cited by 1 · see citing works
Dudek, M., Dębowski, M., Nowicka, A., Kazimierowicz, J., & Zieliński, M. (2022) · Frontiers in Bioscience-Elite · doi:10.31083/j.fbe1401005 · Cited by 1 · see citing works
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Zieliński, M., Barczak, Ł., Rusanowska, P., Kazimierowicz, J., & Dębowski, M. (2024) · Energies · doi:10.3390/en17215287 · Cited by 7 · see citing works
Self-citationZieliński, M., Barczak, Ł., Rusanowska, P., Nowicka, A., & Dębowski, M. (2025) · Energies · doi:10.3390/en18040874 · Cited by 4 · see citing works
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- Authors
- Dębowski, M., Dudek, M., Kazimierowicz, J., Quattrocelli, P., Rusanowska, P., Barczak, Ł., Nowicka, A., & Zieliński, M.
- Affiliations
- University of Warmia and Mazury in Olsztyn (PL)
- Bialystok University of Technology (PL)
- Scuola Superiore Sant'Anna (IT)
- Corresponding author
- Dębowski, Marcin
- ORCID
- Venue
- Energies
- Publisher
- MDPI AG
- Published
- 2024-07-25
- Volume
- 17
- Issue
- 15
- Pages
- 3670
- ISSN
- 1996-1073 (ISSN-L)
- OpenAlex
- W4400975880
- Cited by
- 4
- References
- 63
- Citations by year
- 2025: 3 · 2024: 1
- FWCI
- 0.27
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Energies (publishedVersion, cc-by)
- RePEc: Research Papers in Economics (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Minister of Science and Higher Education (29.610.023-110, WZ/WB-IIŚ/3/2022)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
- Physical Sciences › Chemical Engineering › Catalysis › Catalysts for Methane Reforming
- Keywords
- microalgal biomass, biohydrogen production, bio-oil, methane production, energy gain, anaerobic digestion, microalgae cultivation
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