Cited by 95Animal Nutrition and PhysiologyVeterinary medicine and infectious diseasesBarrier Structure and Function Studies

Effects of Soybean Agglutinin on Intestinal Barrier Permeability and Tight Junction Protein Expression in Weaned Piglets

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Zhao, Yuan, Qin, Guixin, Sun, Zewei, Che, Dongsheng, Bao, Nan, Zhang, Xiaodong·2011·International Journal of Molecular Sciences

Abstract

This study was developed to provide further information on the intestinal barrier permeability and the tight junction protein expression in weaned piglets fed with different levels of soybean agglutinin (SBA). Twenty-five weaned crossbred barrows (Duroc × Landrace × Yorkshire) were selected and randomly allotted to five groups, each group with five replicates. The piglets in the control group were not fed with leguminous products. 0.05, 0.1, 0.15 and 0.2% SBA was added to the control diet to form four experimental diets, respectively. After the experimental period of 7 days (for each group), all the piglets were anesthetized with excess procaine and slaughtered. The D-lactic acid in plasma and the Ileal mucosa diamine oxidase (DAO) was analyzed to observe the change in the intestinal permeability. The tight junction proteins occludin and ZO-1 in the jejunum tissue distribution and relative expression were detected by immunohistochemistry and Western Blot. The results illustrated that a high dose of SBA (0.1–0.2%) could increase the intestinal permeability and reduce piglet intestinal epithelial tight junction protein occludin or ZO-1 expression, while low dose of SBA (0.05% of total diet) had no significant affects. The contents of DAO, D-lactic acid, occludin or ZO-1, had a linear relationship with the SBA levels (0–0.2%) in diets. The high dose SBA (0.1–0.2%) could increase the intestinal permeability and reduce piglet intestinal epithelial tight junction protein occludin or ZO-1 expression, while low dose of SBA (0.05% of total diet) had no affects.

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  • Nonsurgical causes of intestinal hyperpermeability also increase absorption of D-lactate from the intestinal lumen.

    “Nonsurgical causes of intestinal hyperpermeability also increase absorption of D-lactate from the intestinal lumen.63,64”

    From The Gut Microbiome and the Brain (2014) · cites this paper as [63]

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  1. [19]Cereijido M Evolution of ideas on the tight junction Tight Junctions Cereijido M. CRC Press Boca Raton, FL, USA 1992 1 13

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Authors
Zhao, Y., Qin, G., Sun, Z., Che, D., Bao, N., & Zhang, X.
Affiliations
  • Jilin Agricultural University (CN)
  • Jilin University (CN)
Corresponding author
Qin, Guixin
Venue
International Journal of Molecular Sciences
Publisher
MDPI AG
Published
2011-11-29
Volume
12
Issue
12
Pages
8502-8512
ISSN
1422-0067 (ISSN-L)
OpenAlex
W1969812442
Cited by
94
References
28
Citations by year
2026: 5 · 2025: 6 · 2024: 9 · 2023: 9 · 2022: 8 · 2021: 8 · 2020: 12 · 2019: 12 · 2018: 7 · 2017: 5 · 2016: 2 · 2015: 4 · 2014: 2 · 2013: 4 · 2012: 1
FWCI
3.76
Type
journal article
Language
en
Open access
Locations
  • International Journal of Molecular Sciences (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Natural Science Foundation of China (31101719)
  • Jilin Agricultural University
Topics
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
  • Health Sciences › Veterinary › Small Animals › Veterinary medicine and infectious diseases
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
Keywords
soybean agglutinin, intestinal permeability, tight junction proteins, occludin, ZO-1, weaned piglets, diamine oxidase, D-lactate, jejunum, dose-dependent effects
MeSH terms
Animals, Amine Oxidase (Copper-Containing), Ileum, Intestinal Absorption, Intestinal Mucosa, Jejunum, Phytohemagglutinins, Swine, Lactic Acid, Soybean Proteins, Plant Lectins, Occludin, Zonula Occludens-1 Protein

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