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Cited by 110GABA and Rice ResearchProbiotics and Fermented FoodsMicrobial Metabolic Engineering and Bioproduction

A Glutamic Acid-Producing Lactic Acid Bacteria Isolated from Malaysian Fermented Foods

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Zareian, Mohsen, Ebrahimpour, Afshin, Bakar, Fatimah Abu, Mohamed, Abdul Karim Sabo, Forghani, Bita, Ab-Kadir, Mohd Safuan B., Saari, Nazamid·2012·International Journal of Molecular Sciences
DOI 10.3390/ijms13055482PMC PMC3382744
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Abstract

L-glutamaic acid is the principal excitatory neurotransmitter in the brain and an important intermediate in metabolism. In the present study, lactic acid bacteria (218) were isolated from six different fermented foods as potent sources of glutamic acid producers. The presumptive bacteria were tested for their ability to synthesize glutamic acid. Out of the 35 strains showing this capability, strain MNZ was determined as the highest glutamic-acid producer. Identification tests including 16S rRNA gene sequencing and sugar assimilation ability identified the strain MNZ as Lactobacillus plantarum. The characteristics of this microorganism related to its glutamic acid-producing ability, growth rate, glucose consumption and pH profile were studied. Results revealed that glutamic acid was formed inside the cell and excreted into the extracellular medium. Glutamic acid production was found to be growth-associated and glucose significantly enhanced glutamic acid production (1.032 mmol/L) compared to other carbon sources. A concentration of 0.7% ammonium nitrate as a nitrogen source effectively enhanced glutamic acid production. To the best of our knowledge this is the first report of glutamic acid production by lactic acid bacteria. The results of this study can be further applied for developing functional foods enriched in glutamic acid and subsequently γ-amino butyric acid (GABA) as a bioactive compound.

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  • Certain Lactobacillus spp. identified in fermented foods are known to synthesize glutamic acid.

    “certain Lactobacillus spp.identified in fermented foods are also known to synthesize glutamic acid (Zareian et al., 2012).”

    From The Microbiota-Gut-Brain Axis (2019) · cites this paper as Zareian et al., 2012

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Authors
Zareian, M., Ebrahimpour, A., Bakar, F. A., Mohamed, A. K. S., Forghani, B., Ab-Kadir, M. S. B., & Saari, N.
Affiliations
  • Universiti Putra Malaysia (MY)
  • Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia
Corresponding author
Saari, Nazamid
ORCID
Venue
International Journal of Molecular Sciences
Publisher
MDPI AG
Published
2012-05-07
Volume
13
Issue
5
Pages
5482-5497
ISSN
1422-0067 (ISSN-L)
DOI
10.3390/ijms13055482
PMC
PMC3382744
OpenAlex
W2092537502
Cited by
110
References
2
Citations by year
2026: 5 · 2025: 10 · 2024: 10 · 2023: 13 · 2022: 13 · 2021: 14 · 2020: 11 · 2019: 10 · 2018: 6 · 2017: 4 · 2016: 4 · 2015: 2 · 2014: 5 · 2013: 1 · 2012: 2
FWCI
3.66
Type
journal article
Language
en
Open access
Licence
Locations
  • International Journal of Molecular Sciences (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Universiti Putra Malaysia Institutional Repository (Universiti Putra Malaysia) (acceptedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
Ministry of Higher Education, Malaysia (05-10-07-380 FR)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › GABA and Rice Research
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Keywords
glutamic acid production, lactic acid bacteria, Lactobacillus plantarum, fermented foods, glucose utilization, growth-associated production, GABA, functional foods, 16S rRNA identification
MeSH terms
Fermentation, Food Microbiology, Malaysia, RNA, Ribosomal, 16S, Glutamic Acid, Lactic Acid, Lactobacillus plantarum, Lactiplantibacillus plantarum

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